Professor William Colledge University positionProfessor Professor William Colledge is pleased to consider applications from prospective PhD students. DepartmentsDepartment of Physiology, Development and Neuroscience Home pagehttp://www.pdn.cam.ac.uk/staff/c... (personal home page) Research ThemesInterestsMy research group is interested in the neuroendocrine regulation of mammalian fertility using transgenic mice as a model system. Puberty and the regulation of mammalian fertility is controlled by hormonal signalling within the hypothalamus. My research is focussed on the newly identified G-protein coupled receptor, GPR54, and the ligands for this receptor (kisspeptides encoded by the Kiss-1 gene) which regulate the hypothalamic stimulation of the pituitary gonadal axis. Mutations in these molecules cause infertility in humans and mice. We are studying the way in which these proteins are regulated in the hypothalamus and how they are influenced by peripheral hormonal signals and other neuropeptides.  Kisspeptin Neurons in AVPV region of mouse hypothalamus expressing tdTomato fluorescent protein View image full-size (1292x968 pixels) Research Focus
EquipmentCell culture ICV delivery Immunohistochemistry qRT-PCR Collaborators
Associated News ItemsKey publicationsd'Anglemont de Tassigny X, Fagg LA, Dixon JP, Day K, Leitch HG, Hendrick AG, Zahn D, Franceschini I, Caraty A, Carlton MB, Aparicio SA, Colledge WH (2007), “Hypogonadotropic hypogonadism in mice lacking a functional Kiss1 gene.” Proc Natl Acad Sci U S A 104(25):10714-9 Details Messager S, Chatzidaki EE, Ma D, Hendrick AG, Zahn D, Dixon J, Thresher RR, Malinge I, Lomet D, Carlton MB, Colledge WH, Caraty A, Aparicio SA (2005), “Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54” Proc Natl Acad Sci U S A 102:1761-1766 Details Seminara SB, Messager S, Chatzidaki EE, Thresher RR, Acierno JS, Shagoury JK, Bo-Abbas Y, Kuohung W, Schwinof KM, Hendrick AG, Zahn D, Dixon J, Kaiser UB, Slaugenhaupt SA, Gusella JF, O’Rahilly S, Carlton MB, Crowley WF, Aparicio SA, Colledge WH (2003), “The GPR54 gene as a regulator of puberty” N Engl J Med 349:1614-1627 Publications2015Herreboudt AM, Kyle VR, Lawrence J, Doran J, Colledge WH (2015), “Kiss1 mutant placentas show normal structure and function in the mouse.” Placenta 36(1):52-8 Details 2014Grosse J, Heffron H, Burling K, Akhter Hossain M, Habib AM, Rogers GJ, Richards P, Larder R, Rimmington D, Adriaenssens AA, Parton L, Powell J, Binda M, Colledge WH, Doran J, Toyoda Y, Wade JD, Aparicio S, Carlton MB, Coll AP, Reimann F, O'Rahilly S, Gribble FM (2014), “Insulin-like peptide 5 is an orexigenic gastrointestinal hormone.” Proc Natl Acad Sci U S A 111(30):11133-8 Details 2013Colledge WH (2013), “How much kissing is required for fertility?” Endocrinology 154(8):2573-4 Details Colledge WH (2013), “Defending sperm function.” PLoS Genet 9(10):e1003889 Details Colledge WH (2013), “The neuroendocrine regulation of the mammalian reproductive axis. Introduction.” Exp Physiol 98(11):1519-21 Details Colledge WH, Doran J, Mei H (2013), “Model systems for studying kisspeptin signalling: mice and cells.” Adv Exp Med Biol 784:481-503 Details Mei H, Doran J, Kyle V, Yeo SH, Colledge WH (2013), “Does Kisspeptin Signaling have a Role in the Testes?” Front Endocrinol (Lausanne) 4:198 Details Stoneking CJ, Shivakumar O, Thomas DN, Colledge WH, Mason MJ (2013), “Voltage dependence of the Ca(2+)-activated K(+) channel K(Ca)3.1 in human erythroleukemia cells.” Am J Physiol Cell Physiol 304(9):C858-72 Details 2012Hanchate NK, Parkash J, Bellefontaine N, Mazur D, Colledge WH, d'Anglemont de Tassigny X, Prevot V (2012), “Kisspeptin-GPR54 signaling in mouse NO-synthesizing neurons participates in the hypothalamic control of ovulation.” J Neurosci 32(3):932-45 Details Prömel S, Waller-Evans H, Dixon J, Zahn D, Colledge WH, Doran J, Carlton MB, Grosse J, Schöneberg T, Russ AP, Langenhan T (2012), “Characterization and functional study of a cluster of four highly conserved orphan adhesion-GPCR in mouse.” Dev Dyn 241(10):1591-602 Details 2011Liu X, Porteous R, d'Anglemont de Tassigny X, Colledge WH, Millar R, Petersen SL, Herbison AE (2011), “Frequency-dependent recruitment of fast amino acid and slow neuropeptide neurotransmitter release controls gonadotropin-releasing hormone neuron excitability.” J Neurosci 31(7):2421-30 Details Maguire JJ, Kirby HR, Mead EJ, Kuc RE, d'Anglemont de Tassigny X, Colledge WH, Davenport AP (2011), “Inotropic action of the puberty hormone kisspeptin in rat, mouse and human: cardiovascular distribution and characteristics of the kisspeptin receptor.” PLoS One 6(11):e27601 Details Mei H, Walters C, Carter R, Colledge WH (2011), “Gpr54-/- mice show more pronounced defects in spermatogenesis than Kiss1-/- mice and improved spermatogenesis with age when exposed to dietary phytoestrogens.” Reproduction 141(3):357-66 Details Porteous R, Petersen SL, Yeo SH, Bhattarai JP, Ciofi P, de Tassigny XD, Colledge WH, Caraty A, Herbison AE (2011), “Kisspeptin neurons co-express met-enkephalin and galanin in the rostral periventricular region of the female mouse hypothalamus.” J Comp Neurol 519(17):3456-69 Details Prentice LM, d'Anglemont de Tassigny X, McKinney S, Ruiz de Algara T, Yap D, Turashvili G, Poon S, Sutcliffe M, Allard P, Burleigh A, Fee J, Huntsman DG, Colledge WH, Aparicio SA (2011), “The testosterone-dependent and independent transcriptional networks in the hypothalamus of Gpr54 and Kiss1 knockout male mice are not fully equivalent.” BMC Genomics 12:209 Details Spampinato S, Trabucco A, Biasiotta A, Biagioni F, Cruccu G, Copani A, Colledge WH, Sortino MA, Nicoletti F, Chiechio S (2011), “Hyperalgesic activity of kisspeptin in mice.” Mol Pain 7:90 Details 2010Colledge WH, Mei H, d'Anglemont de Tassigny X (2010), “Mouse models to study the central regulation of puberty.” Mol Cell Endocrinol 324(1-2):12-20 Details d'Anglemont de Tassigny X, Ackroyd KJ, Chatzidaki EE, Colledge WH (2010), “Kisspeptin signaling is required for peripheral but not central stimulation of gonadotropin-releasing hormone neurons by NMDA.” J Neurosci 30(25):8581-90 Details d'Anglemont de Tassigny X, Colledge WH (2010), “The role of kisspeptin signaling in reproduction.” Physiology (Bethesda) 25(4):207-17 Details Herbison AE, de Tassigny X, Doran J, Colledge WH (2010), “Distribution and postnatal development of Gpr54 gene expression in mouse brain and gonadotropin-releasing hormone neurons.” Endocrinology 151(1):312-21 Details Kirby HR, Maguire JJ, Colledge WH, Davenport AP (2010), “International Union of Basic and Clinical Pharmacology. LXXVII. Kisspeptin receptor nomenclature, distribution, and function.” Pharmacol Rev 62(4):565-78 Details 2009Clarkson J, d'Anglemont de Tassigny X, Colledge WH, Caraty A, Herbison AE (2009), “Distribution of kisspeptin neurones in the adult female mouse brain.” J Neuroendocrinol 21(8):673-82 Details Colledge WH (2009), “Kisspeptins and GnRH neuronal signalling.” Trends Endocrinol Metab 20(3):115-21 Details Colledge WH (2009), “Transgenic mouse models to study Gpr54/kisspeptin physiology.” Peptides 30(1):34-41 Details 2008Clarkson J, d'Anglemont de Tassigny X, Moreno AS, Colledge WH, Herbison AE (2008), “Kisspeptin-GPR54 signaling is essential for preovulatory gonadotropin-releasing hormone neuron activation and the luteinizing hormone surge.” J Neurosci 28(35):8691-7 Details Colledge WH (2008), “GPR54 and kisspeptins.” Results Probl Cell Differ 46:117-43 Details d'Anglemont de Tassigny X, Fagg LA, Carlton MB, Colledge WH (2008), “Kisspeptin can stimulate gonadotropin-releasing hormone (GnRH) release by a direct action at GnRH nerve terminals.” Endocrinology 149(8):3926-32 Details 2003Seminara SB, Messager S, Chatzidaki EE, Thresher RR, Acierno JS Jr, Shagoury JK, Bo-Abbas Y, Kuohung W, Schwinof KM, Hendrick AG, Zahn D, Dixon J, Kaiser UB, Slaugenhaupt SA, Gusella JF, O'Rahilly S, Carlton MB, Crowley WF Jr, Aparicio SA, Colledge WH (2003), “The GPR54 gene as a regulator of puberty.” N Engl J Med 349(17):1614-27 Details |
