Dr Toshiaki ShigeokaUniversity positionResearch Associate DepartmentsDepartment of Physiology, Development and Neuroscience Research ThemesInterestsRNA transport and local translation play key roles in the formation and function of neural network. Recent in vitro studies provide evidence that the local translation in axon is critical for the axon pathfinding during development. However, because of the paucity of in vivo experimental tools, it is largely unknown whether the axonal protein synthesis contributes to later neuronal events, such as synaptogenesis and neurotransmission. I am studying the axonal translation and its regulatory mechanisms in vivo by using a novel method, Axon-TRAP that allows us to isolate ribosome-bound mRNAs from mouse distal retinal axons. Research Focus
EquipmentCell culture Fluorescence microscopy Immunohistochemistry Microscopy Protein purification CollaboratorsNo collaborators listed Associated News ItemsPublications2013Shigeoka T, Lu B, Holt CE (2013), “Cell biology in neuroscience: RNA-based mechanisms underlying axon guidance.” J Cell Biol 202(7):991-9 Details 2012Mayasari NI, Mukougawa K, Shigeoka T, Kawakami K, Kawaichi M, Ishida Y (2012), “Mixture of differentially tagged Tol2 transposons accelerates conditional disruption of a broad spectrum of genes in mouse embryonic stem cells.” Nucleic Acids Res 40(13):e97 Details Shigeoka T, Kato S, Kawaichi M, Ishida Y (2012), “Evidence that the Upf1-related molecular motor scans the 3'-UTR to ensure mRNA integrity.” Nucleic Acids Res 40(14):6887-97 Details 2005Shigeoka T, Kawaichi M, Ishida Y (2005), “Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.” Nucleic Acids Res 33(2):e20 Details 2004Matsuda E, Shigeoka T, Iida R, Yamanaka S, Kawaichi M, Ishida Y (2004), “Expression profiling with arrays of randomly disrupted genes in mouse embryonic stem cells leads to in vivo functional analysis.” Proc Natl Acad Sci U S A 101(12):4170-4 Details |