Dr Richard Eva

Richard Eva

University position

Senior Research Associate

Departments

Department of Clinical Neurosciences

Institutes

Cambridge Centre for Brain Repair

Home page

https://www.researchgate.net/profile... (personal home page)

Research Theme

Cellular and Molecular Neuroscience

Interests

Investigating the neuron-intrinsic mechanisms regulating axon regeneration in the central nervous system, in order to develop treatments for repairing damage caused by disease or injury. As CNS neurons develop, they lose their intrinsic capacity for axon growth, and I am trying to understand why. I use laser axotomy, models of CNS injury and live cell imaging to study the developmental regulation of axon transport and regeneration. Enhancing the axonal transport of growth machinery is our target for enhancing brain, spinal cord and optic nerve regeneration. I have identified Rab11 and ARF6 as the central regulators of regenerative transport, and have grants from the MRC, Fight for Sight and NERC to continue this work.

Growth Cone of an adult DRG axon expressing Rab Coupling protein GFP and labelled for beta1 integrin
Axon regeneration in the optice nerve
Click image to view full-size

Research Focus

Keywords

Neuronal Cell Biology

Axon regeneration

Organelle Traffic

Axon Transport

Neuronal Survival

Clinical conditions

spinal cord injury, axon regeneration, neurodegenerative disease

Equipment

Cell culture

Confocal microscopy

Fluorescence microscopy

Neuronal cell culture, Compartmentalised neuronal culture, In vitro axonal injury (laser axotomy), Live cell imaging, Spinning disc microscopy, Neurite outgrowth quantification, Generation of stable cell lines, siRNA treatment, Neuronal transfection (by multiple methods), Subcellular fractionation, Capture elisa, Biochemistry, Axon transport analysis, Growth cone morphology analysis, Membrane protein endocytosis and recycling assays, Gene cloning, Site directed mutagenesis,

Recombinant protein expression

Collaborators

Cambridge

Michael Coleman

Phill Hawkins

Keith Martin

Klaus Okkenhaug

United Kingdom

Venkateswarlu Kanamarlapudi Web: http://www.swan.ac.uk/

Jim Norman Web: http://www.beatson.gla.ac.uk/Regulat...

International

Britta Eickholt Web: http://www.neurosignallab.com

Pete Williams Web: https://petewilliamslab.com/

Associated News Items


Publications

2018

Nieuwenhuis B, Eva R (2018), “Linking axon transport to regeneration using in vitro laser axotomy ” Neural Regeneration Research 13(3): 410-412 PDF

Nieuwenhuis B, Eva R (2018), “ARF6 and Rab11 as intrinsic regulators of axon regeneration” Small GTPases May 17:1-10. PDF

Petrova V, Eva, R (2018), “The virtuous cycle of axon growth: Axonal transport of growth‐promoting machinery as an intrinsic determinant of axon regeneration” Developmental Neurobiology July 10 [Epub ahead of print]

2017

Eva, R., Koseki, H., Kanamarlapudi, V., Fawcett, J.W. (2017), “EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment. ” Journal of Cell Science 130(21)2018 13(3): 3663-3675

Koseki, H., Donegá, M., Lam, B.Y.H., Petrova, V., Yeo, G.S.H., Kwok, J.C.F., van Erp, S., ffrench-Constant, C., Eva, R. *, Fawett, J.W. *. *Joint corresponding author. (2017), “Selective Rab11 transport and the intrinsic regenerative ability of CNS axons. ” eLife 6, e26956.

2016

Heintz TG, Eva R, Fawcett JW. (2016), “Regional Regulation of Purkinje Cell Dendritic Spines by Integrins and Eph/Ephrins. ” PLoS One 11(8):e0158558

2015

Franssen EH, Zhao RR, Koseki H, Kanamarlapudi V, Hoogenraad CC, Eva R, Fawcett JW (2015), “Exclusion of Integrins from CNS Axons Is Regulated by Arf6 Activation and the AIS.” J Neurosci 35(21):8359-75 Details

Tan CL, Kwok JC, Heller JP, Zhao R, Eva R, Fawcett JW (2015), “Full length talin stimulates integrin activation and axon regeneration.” Mol Cell Neurosci 68:1-8 Details

2014

Eva R, Fawcett J (2014), “Integrin signalling and traffic during axon growth and regeneration.” Curr Opin Neurobiol 27C:179-185 Details

Heintz TG, Heller J, Zhao R, Caceres A, Eva R, Fawcett JW (2014), “Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons.” Mol Cell Neurosci Details

2012

Eva R, Andrews MR, Franssen EH, Fawcett JW (2012), “Intrinsic mechanisms regulating axon regeneration: an integrin perspective.” Int Rev Neurobiol 106:75-104 Details

Eva R, Bouyoucef-Cherchalli D, Patel K, Cullen PJ, Banting G (2012), “IP3 3-kinase opposes NGF driven neurite outgrowth.” PLoS One 7(2):e32386 Details

Eva R, Crisp S, Marland JR, Norman JC, Kanamarlapudi V, Ffrench-Constant C, Fawcett JW (2012), “ARF6 Directs Axon Transport and Traffic of Integrins and Regulates Axon Growth in Adult DRG Neurons.” J Neurosci 32(30):10352-10364 Details

2010

Eva R, Dassie E, Caswell PT, Dick G, ffrench-Constant C, Norman JC, Fawcett JW (2010), “Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells.” J Neurosci 30(35):11654-69 Details