Dr Richard Eva University positionSenior Research Associate DepartmentsDepartment of Clinical Neurosciences InstitutesCambridge Centre for Brain Repair Home pagehttps://www.researchgate.net/profile... (personal home page) Research ThemeInterestsInvestigating the neuron-intrinsic mechanisms regulating axon regeneration in the central nervous system, in order to develop treatments for repairing damage caused by disease or injury. As CNS neurons develop, they lose their intrinsic capacity for axon growth, and I am trying to understand why. I use laser axotomy, models of CNS injury and live cell imaging to study the developmental regulation of axon transport and regeneration. Enhancing the axonal transport of growth machinery is our target for enhancing brain, spinal cord and optic nerve regeneration. I have identified Rab11 and ARF6 as the central regulators of regenerative transport, and have grants from the MRC, Fight for Sight and NERC to continue this work.  Axon regeneration in the optice nerve Click image to view full-size Research Focus
EquipmentCell culture Confocal microscopy Fluorescence microscopy Neuronal cell culture, Compartmentalised neuronal culture, In vitro axonal injury (laser axotomy), Live cell imaging, Spinning disc microscopy, Neurite outgrowth quantification, Generation of stable cell lines, siRNA treatment, Neuronal transfection (by multiple methods), Subcellular fractionation, Capture elisa, Biochemistry, Axon transport analysis, Growth cone morphology analysis, Membrane protein endocytosis and recycling assays, Gene cloning, Site directed mutagenesis, Recombinant protein expression Collaborators
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Publications2020Nieuwenhuis B, Barber AC, Evans RS, Pearson CS, Fuchs J, MacQueen AR, van Erp S, Haenzi B, Hulshof LA, Osborne A, Conceicao R, Khatib TZ, Deshpande SS, Cave J, ffrench-Constant C, Smith PD, Okkenhaug K, Eickholt BJ, Martin KR, Fawcett JW, Eva R (2020), “PI 3‐kinase delta enhances axonal PIP3 to support axon regeneration in the adult CNS ” EMBO Mol Med e11674 in pressPetrova V, Pearson CS, Ching J, Tribble JR, Solano AG, Yang Y, Love FM, Watt RJ, Osborne A, Reid E, Williams, PA, Martin KR, Geller HM, Eva R*, Fawcett JW*, * Joint Senior Author (in press), “Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS” Nature Communications 2018Nieuwenhuis B, Eva R (2018), “Linking axon transport to regeneration using in vitro laser axotomy ” Neural Regeneration Research 13(3): 410-412 PDF Nieuwenhuis B, Eva R (2018), “ARF6 and Rab11 as intrinsic regulators of axon regeneration” Small GTPases May 17:1-10. PDF Petrova V, Eva, R (2018), “The virtuous cycle of axon growth: Axonal transport of growth‐promoting machinery as an intrinsic determinant of axon regeneration” Developmental Neurobiology July 10 [Epub ahead of print] 2017Eva, R., Koseki, H., Kanamarlapudi, V., Fawcett, J.W. (2017), “EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment. ” Journal of Cell Science 130(21)2018 13(3): 3663-3675 Koseki, H., Donegá, M., Lam, B.Y.H., Petrova, V., Yeo, G.S.H., Kwok, J.C.F., van Erp, S., ffrench-Constant, C., Eva, R. *, Fawett, J.W. *. *Joint corresponding author. (2017), “Selective Rab11 transport and the intrinsic regenerative ability of CNS axons. ” eLife 6, e26956. 2016Heintz TG, Eva R, Fawcett JW. (2016), “Regional Regulation of Purkinje Cell Dendritic Spines by Integrins and Eph/Ephrins. ” PLoS One 11(8):e0158558 2015Franssen EH, Zhao RR, Koseki H, Kanamarlapudi V, Hoogenraad CC, Eva R, Fawcett JW (2015), “Exclusion of Integrins from CNS Axons Is Regulated by Arf6 Activation and the AIS.” J Neurosci 35(21):8359-75 Details Tan CL, Kwok JC, Heller JP, Zhao R, Eva R, Fawcett JW (2015), “Full length talin stimulates integrin activation and axon regeneration.” Mol Cell Neurosci 68:1-8 Details 2014Eva R, Fawcett J (2014), “Integrin signalling and traffic during axon growth and regeneration.” Curr Opin Neurobiol 27C:179-185 Details Heintz TG, Heller J, Zhao R, Caceres A, Eva R, Fawcett JW (2014), “Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons.” Mol Cell Neurosci Details 2012Eva R, Andrews MR, Franssen EH, Fawcett JW (2012), “Intrinsic mechanisms regulating axon regeneration: an integrin perspective.” Int Rev Neurobiol 106:75-104 Details Eva R, Bouyoucef-Cherchalli D, Patel K, Cullen PJ, Banting G (2012), “IP3 3-kinase opposes NGF driven neurite outgrowth.” PLoS One 7(2):e32386 Details Eva R, Crisp S, Marland JR, Norman JC, Kanamarlapudi V, Ffrench-Constant C, Fawcett JW (2012), “ARF6 Directs Axon Transport and Traffic of Integrins and Regulates Axon Growth in Adult DRG Neurons.” J Neurosci 32(30):10352-10364 Details 2010Eva R, Dassie E, Caswell PT, Dick G, ffrench-Constant C, Norman JC, Fawcett JW (2010), “Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells.” J Neurosci 30(35):11654-69 Details |