Dr Richard Eva University positionResearch Associate DepartmentsDepartment of Clinical Neurosciences InstitutesCambridge Centre for Brain Repair Home pagehttp://www.brc.cam.ac.uk/princip... Research ThemeInterestsBoosting axon regeneration after injury by targeting axon transport of growth receptors I would like to understand the mechanisms regulating the traffic and transport of growth and survival molecules into axons. During development these molecules are present, but either decline or are absent as neurons mature and age. Consequently, injured or diseased axons in the central nervous system do not regenerate after injury. Additionally, numerous neurological diseases are associated with deficits in axonal transport, and may be caused by defects in intracellular trafficking. I have identified and characterised axonal trafficking pathways that can be manipulated to enhance axon growth and regeneration via the increased transport of growth associated molecules into axons. I am continuing to characterise newly identified signalling and trafficking pathways, and manipulating these pathways in order to increase axon regeneration after injury.  Growth Cone of an adult DRG axon expressing Rab Coupling protein GFP and labelled for beta1 integrin Click image to view full-size Research Focus
EquipmentCell culture Confocal microscopy Fluorescence microscopy Neuronal cell culture, Compartmentalised neuronal culture, In vitro axonal injury (laser axotomy), Live cell imaging, Spinning disc microscopy, Neurite outgrowth quantification, Generation of stable cell lines, siRNA treatment, Neuronal transfection (by multiple methods), Subcellular fractionation, Capture elisa, Biochemistry, Axon transport analysis, Growth cone morphology analysis, Membrane protein endocytosis and recycling assays, Gene cloning, Site directed mutagenesis, Recombinant protein expression Collaborators
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Key publicationsEva, R., Koseki, H., Kanamarlapudi, V., Fawcett, J.W. (2017), “EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment. ” Journal of Cell Science 130(21)2018 13(3): 3663-3675 Koseki, H., Donegá, M., Lam, B.Y.H., Petrova, V., Yeo, G.S.H., Kwok, J.C.F., van Erp, S., ffrench-Constant, C., Eva, R. *, Fawett, J.W. *. *Joint corresponding author. (2017), “Selective Rab11 transport and the intrinsic regenerative ability of CNS axons. ” eLife 6, e26956. Franssen EH, Zhao RR, Koseki H, Kanamarlapudi V, Hoogenraad CC, Eva R, Fawcett JW (2015), “Exclusion of Integrins from CNS Axons Is Regulated by Arf6 Activation and the AIS.” J Neurosci 35(21):8359-75 Details Eva R, Crisp S, Marland JR, Norman JC, Kanamarlapudi V, Ffrench-Constant C, Fawcett JW (2012), “ARF6 Directs Axon Transport and Traffic of Integrins and Regulates Axon Growth in Adult DRG Neurons.” J Neurosci 32(30):10352-10364 Details Eva R, Dassie E, Caswell PT, Dick G, ffrench-Constant C, Norman JC, Fawcett JW (2010), “Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells.” J Neurosci 30(35):11654-69 Details Publications2018Nieuwenhuis B, Eva R (2018), “Linking axon transport to regeneration using in vitro laser axotomy ” Neural Regeneration Research 13(3): 410-412 PDF Nieuwenhuis B, Eva R (2018), “ARF6 and Rab11 as intrinsic regulators of axon regeneration” Small GTPases PDF 2016Heintz TG, Eva R, Fawcett JW. (2016), “Regional Regulation of Purkinje Cell Dendritic Spines by Integrins and Eph/Ephrins. ” PLoS One 11(8):e0158558 2015Tan CL, Kwok JC, Heller JP, Zhao R, Eva R, Fawcett JW (2015), “Full length talin stimulates integrin activation and axon regeneration.” Mol Cell Neurosci 68:1-8 Details 2014Eva R, Fawcett J (2014), “Integrin signalling and traffic during axon growth and regeneration.” Curr Opin Neurobiol 27C:179-185 Details Heintz TG, Heller J, Zhao R, Caceres A, Eva R, Fawcett JW (2014), “Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons.” Mol Cell Neurosci Details 2012Eva R, Andrews MR, Franssen EH, Fawcett JW (2012), “Intrinsic mechanisms regulating axon regeneration: an integrin perspective.” Int Rev Neurobiol 106:75-104 Details Eva R, Bouyoucef-Cherchalli D, Patel K, Cullen PJ, Banting G (2012), “IP3 3-kinase opposes NGF driven neurite outgrowth.” PLoS One 7(2):e32386 Details |