Dr Richard Shannon University positionResearch Assistant DepartmentsDepartment of Clinical Neurosciences InstitutesHome pagehttp://www.neurosurg.cam.ac.uk/ Research ThemeInterestsIn the neurosurgery laboratory we use analytical techniques such as HPLC, ELISA and Luminex Multiplex to measure levels of chemicals and proteins in brain microdialysate samples. Brain microdialysis is used in the neurocritical care unit (NCCU) to help care for patients with severe TBI. On the NCCU the microdialysis analytes of interest are glucose, lactate, pyruvate, glycrol and glutamate. The microdialysis samples can then be analysed for other substances in the lab. Research Focus
EquipmentELISA FPLC/HPLC Luminex Multiplex Nitrogen oxide analyser CollaboratorsNo collaborators listed Associated News ItemsKey publicationsShannon RJ, Timofeev I, Nortje J, Hutchinson PJ, Carpenter KL (2014), “Monitoring vigabatrin in head injury patients by cerebral microdialysis: obtaining pharmacokinetic measurements in a neuro-critical care setting.” Br J Clin Pharmacol Details Publications2014Jalloh I, Helmy A, Shannon RJ, Gallagher CN, Menon D, Carpenter K, Hutchinson P (2014), “Response to letter 'Lactate uptake against a concentration gradient - misinterpretation of analytical imprecision.'” J Neurotrauma Details 2013Jalloh I, Helmy A, Shannon RJ, Gallagher CN, Menon DK, Carpenter KL, Hutchinson PJ (2013), “Lactate uptake by the injured human brain: evidence from an arteriovenous gradient and cerebral microdialysis study.” J Neurotrauma 30(24):2031-7 Details Shannon RJ, Carpenter KL, Guilfoyle MR, Helmy A, Hutchinson PJ (2013), “Cerebral microdialysis in clinical studies of drugs: pharmacokinetic applications.” J Pharmacokinet Pharmacodyn 40(3):343-58 Details 2006Carroll J, Fearnley IM, Skehel JM, Shannon RJ, Hirst J, Walker JE (2006), “Bovine complex I is a complex of 45 different subunits.” J Biol Chem 281(43):32724-7 Details Marshall D, Fisher N, Grigic L, Zickermann V, Brandt U, Shannon RJ, Hirst J, Lawrence R, Rich PR (2006), “ATR-FTIR redox difference spectroscopy of Yarrowia lipolytica and bovine complex I.” Biochemistry 45(17):5458-67 Details Sharpley MS, Shannon RJ, Draghi F, Hirst J (2006), “Interactions between phospholipids and NADH:ubiquinone oxidoreductase (complex I) from bovine mitochondria.” Biochemistry 45(1):241-8 Details 2005Carroll J, Fearnley IM, Skehel JM, Runswick MJ, Shannon RJ, Hirst J, Walker JE (2005), “The post-translational modifications of the nuclear encoded subunits of complex I from bovine heart mitochondria.” Mol Cell Proteomics 4(5):693-9 Details 2003Carroll J, Fearnley IM, Shannon RJ, Hirst J, Walker JE (2003), “Analysis of the subunit composition of complex I from bovine heart mitochondria.” Mol Cell Proteomics 2(2):117-26 Details Hirst J, Carroll J, Fearnley IM, Shannon RJ, Walker JE (2003), “The nuclear encoded subunits of complex I from bovine heart mitochondria.” Biochim Biophys Acta 1604(3):135-50 Details Taylor ER, Hurrell F, Shannon RJ, Lin TK, Hirst J, Murphy MP (2003), “Reversible glutathionylation of complex I increases mitochondrial superoxide formation.” J Biol Chem 278(22):19603-10 Details Zu Y, Shannon RJ, Hirst J (2003), “Reversible, electrochemical interconversion of NADH and NAD+ by the catalytic (Ilambda) subcomplex of mitochondrial NADH:ubiquinone oxidoreductase (complex I).” J Am Chem Soc 125(20):6020-1 Details 2002Carroll J, Shannon RJ, Fearnley IM, Walker JE, Hirst J (2002), “Definition of the nuclear encoded protein composition of bovine heart mitochondrial complex I. Identification of two new subunits.” J Biol Chem 277(52):50311-7 Details 1996Hunter CA, Shannon RJ (1996), “. Photoinduced electron transfer on a supramolecular scaffold” Chemical Communications 11:1361-2 1995Brooksby PA, Hunter CA, Mcquillan AJ, Purvis DH, Rowan AE, Shannon RJ, Walsh R (1995), “Supramolecular Activation of Para-Benzoquinone” Angewandte Chemie-International Edition in English 33(23-24):2489-91 1994Carver FJ, Hunter CA, Shannon RJ (1994), “Directed Macrocyclization Reactions” Journal of the Chemical Society-Chemical Communications 10:1277-80 |