Professor Roger Thomas

Interests

Intracellular ion homeostasis in nerve cells. I use pH and Ca2+ sensitive microelectrodes to study ionic interactions inside large snail neurones. I am currently investigating the Ca:H coupling ratio of the plasma membrane Ca pump, or PMCA.

The effect of injecting HCl on the intracellular Ca of a snail neurone
The effect of injecting HCl on the intracellular Ca of a snail neurone
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Research Focus

Keywords

Intracellular pH

intracellular Ca2+

PMCA

Clinical conditions

No direct clinical relevance

Equipment

Electrophysiological recording techniques

Intracellular recording

Ion-sensitive microelectrodes

Iontophoretic injections

Whole cell patch clamp

Collaborators

Cambridge

Christof Schwiening

Associated News Items


    Key publications

    Thomas RC (2011), “The Ca(2+):H(+) coupling ratio of the plasma membrane calcium ATPase in neurones is little sensitive to changes in external or internal pH.” Cell Calcium 49(6):357-64 Details

    Thomas RC (2009), “The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and exchanges 2 H+ for each Ca2+ or Ba2+ ion extruded. ” J Physiol 587.2 pp 315327

    Thomas RC, Postma M (2007), “Dynamic and static calcium gradients inside large snail (Helix aspersa) neurones detected with calcium-sensitive microelectrodes” Cell Calcium 41:365-378 Details

    Thomas RC (2002), “The effects of HCl and CaCl2 injections on intracellular calcium and pH in voltage-clamped snail (Helix aspersa) neurons” J Gen Physiol 120: 567-579

    Publications

    2013

    Thomas RC (2013), “Calcium content of the endoplasmic reticulum of snail neurones releasable by caffeine.” Cell Calcium 53(2):120-4 Details

    Thomas RC, Bers DM (2013), “Calcium-sensitive mini- and microelectrodes.” Cold Spring Harb Protoc 2013(4):305-9 Details

    Thomas RC, Bers DM (2013), “How to make calcium-sensitive microelectrodes.” Cold Spring Harb Protoc 2013(4):374-8 Details

    Thomas RC, Bers DM (2013), “How to make calcium-sensitive minielectrodes.” Cold Spring Harb Protoc 2013(4):370-3 Details

    2009

    Thomas RC (2009), “The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and exchanges 2 H+ for each Ca2+ or Ba2+ ion extruded.” J Physiol 587(Pt 2):315-27 Details

    2003

    Thomas RC, Pagnotta SE, Nistri A (2003), “Whole-cell recording of intracellular pH with silanized and oiled patch-type single or double-barreled microelectrodes.” Pflugers Arch 447(2):259-65 Details

    2002

    Thomas RC (2002), “The effects of HCl and CaCl(2) injections on intracellular calcium and pH in voltage-clamped snail (Helix aspersa) neurons.” J Gen Physiol 120(4):567-79 Details

    2001

    Collins RO, Thomas RC (2001), “The effect of calcium pump inhibitors on the response of intracellular calcium to caffeine in snail neurones.” Cell Calcium 30(1):41-8 Details

    1999

    Willoughby D, Thomas RC, Schwiening CJ (1999), “A role for Na+/H+ exchange in pH regulation in Helix neurones.” Pflugers Arch 438(6):741-9 Details

    1998

    Willoughby D, Thomas RC, Schwiening CJ (1998), “Comparison of simultaneous pH measurements made with 8-hydroxypyrene-1,3,6-trisulphonic acid (HPTS) and pH-sensitive microelectrodes in snail neurones.” Pflugers Arch 436(4):615-22 Details