Dr Stefano Pluchino

Stefano Pluchino

University position


Dr Stefano Pluchino is pleased to consider applications from prospective PhD students.


Department of Clinical Neurosciences


Cambridge Centre for Brain Repair and MRC Cambridge Stem Cell Institute



Home page

http://pluchino.brc.cam.ac.uk/labwiki... (personal home page)

Research Themes

Cellular and Molecular Neuroscience

Systems and Computational Neuroscience


Recent evidence from our own laboratory indicates that the systemic injection of somatic neural stem/precursor cells (NPCs) very efficiently protect the CNS from the chronic degeneration induced by inflammation both in small rodents as well as in non-human primates affected by experimental MS, cerebral ischemic stroke, or contusion spinal cord injury (stem cell therapeutic plasticity). We are now exploring the cellular and molecular mechanisms regulating the therapeutic plasticity of stem cells in pre-clinical models of complex CNS diseases such as multiple sclerosis, and spinal cord injury.

We are devoting special attention to the different modalities by which NPCs engage programs of horizontal cell-to-cell communication with cells in the microenvironment.

Our major aim is to look into innate (physiological) mechanisms with the visionary focus of translating the knowledge of basal (vs reactive) stem cell functions into innovative high clinical impact therapeutics.

Confocal microscopy image of two intravenously-injected ?-gal+ NPCs (green) persisting (110 days after cell injection) at the level of a representative CNS perivascular area from a R-EAE mouse in very close contact to CNS-infiltrating encephalitogenic blood-borne CD45+ inflammatory cells (red) [Nature 2005; 436: 266-71].
Confocal microscopy image of two intravenously-injected ?-gal+ NPCs (green) persisting (110 days after cell injection) at the level of a representative CNS perivascular area from a R-EAE mouse in very close contact to CNS-infiltrating encephalitogenic blood-borne CD45+ inflammatory cells (red) [Nature 2005; 436: 266-71].
Click image to view full-size

Research Focus


Somatic neural stem cells

Pluripotent stem cells


Secreted membrane vesicles

Small regulatory RNAs

Clinical conditions

Autoimmune diseases

Cerebrovascular disorders


Multiple sclerosis

Spinal cord injuries


Traumatic brain injury


Cell culture

Cell transplantation

Computational modelling

Confocal microscopy

Electron microscopy (scanning and transmission)

Flow cytometry

Fluorescence microscopy

hES cells


In situ hybridization

In vitro co-cultures

Magnetic resonance imaging (MRI)

Mathematical modeling


Non viral delivery technologies (nanoparticles; liposomes)

Reverse engineering

RNA deep sequencing

Semi-quantitative real time RT-PCR


Unbiased stereology

Viral vector technology for cell engineering and ncRNA delivery



Sabine Dietmann

Robin Franklin

United Kingdom

Manolis Fanto Web: http://www.kcl.ac.uk/depsta/...


Angela Bachi Web: http://www.sanraffaele.org/EN_home...

Fabio Biscarini Web: http://www.bo.ismn.cnr.it/staff.p...

Michele DePalma Web: https://www.sanraffaele.org/MDePalm...

Jose-Manuel Garcia-Verdugo Web: http://www.uv.es/garciajm/

Samia Khoury Web: http://khourylab.bwh.harvard.edu/

Yang Mao-Draayer Web: http://www.med.uvm.edu/Neurolo...

John Mattick Web: http://jsm-research.imb.uq.edu.au/groupwi...

Pietro Mortini Web: http://www.mortini.net

Giovanna Musco Web: http://www.sanraffaele.org/home/ri...

Luigi Naldini Web: http://www.unisr.it/persona...

Alessandro Sannino Web: http://bioslab.unile.it

Bert t'Hart Web: http://www.bprc.nl/

Alexey Terkisk Web: http://www.burnham.org/default...

Mark West Web: http://person.au.dk/en/mjw@...

Associated News Items

Key publications

Cusimano M, Biziato D, Brambilla E, Donegŕ M, Alfaro-Cervello C, Snider S, Salani G, Pucci F, Comi G, Garcia-Verdugo JM, De Palma M, Martino G, Pluchino S (2012), “Transplanted neural stem/precursor cells instruct phagocytes and reduce secondary tissue damage in the injured spinal cord.” Brain Details

Bacigaluppi M, Pluchino S, Peruzzotti Jametti L, Kilic E, Kilic U, Salani G, Brambilla E, West MJ, Comi G, Martino G, Hermann DM (2009), “Delayed post-ischaemic neuroprotection following systemic neural stem cell transplantation involves multiple mechanisms.” Brain 132(Pt 8):2239-51 Details

Pluchino S, Gritti A, Blezer E, Amadio S, Brambilla E, Borsellino G, Cossetti C, Del Carro U, Comi G, 't Hart B, Vescovi A, Martino G (2009), “Human neural stem cells ameliorate autoimmune encephalomyelitis in non-human primates.” Ann Neurol 66(3):343-54 Details

Pluchino S, Zanotti L, Brambilla E, Rovere-Querini P, Capobianco A, Alfaro-Cervello C, Salani G, Cossetti C, Borsellino G, Battistini L, Ponzoni M, Doglioni C, Garcia-Verdugo JM, Comi G, Manfredi AA, Martino G (2009), “Immune regulatory neural stem/precursor cells protect from central nervous system autoimmunity by restraining dendritic cell function.” PLoS One 4(6):e5959 Details

Pluchino S, Muzio L, Imitola J, Deleidi M, Alfaro-Cervello C, Salani G, Porcheri C, Brambilla E, Cavasinni F, Bergamaschi A, Garcia-Verdugo JM, Comi G, Khoury SJ, Martino G (2008), “Persistent inflammation alters the function of the endogenous brain stem cell compartment.” Brain 131(Pt 10):2564-78 Details

Pluchino S, Zanotti L, Rossi B, Brambilla E, Ottoboni L, Salani G, Martinello M, Cattalini A, Bergami A, Furlan R, Comi G, Constantin G, Martino G (2005), “Neurosphere-derived multipotent precursors promote neuroprotection by an immunomodulatory mechanism.” Nature 436(7048):266-71 Details

Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G. (2003), “Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis.” Nature Apr 17;422(6933):688-94



Cerri F, Salvatore L, Memon D, Martinelli Boneschi F, Madaghiele M, Brambilla P, Del Carro U, Taveggia C, Riva N, Trimarco A, Lopez ID, Comi G, Pluchino S, Martino G, Sannino A, Quattrini A (2014), “Peripheral nerve morphogenesis induced by scaffold micropatterning.” Biomaterials 35(13):4035-45 Details

Donegŕ M, Giusto E, Cossetti C, Schaeffer J, Pluchino S (2014), “Systemic injection of neural stem/progenitor cells in mice with chronic EAE.” J Vis Exp (86) Details

L'Episcopo F, Tirolo C, Caniglia S, Testa N, Morale MC, Serapide MF, Pluchino S, Marchetti B (2014), “Targeting Wnt signaling at the neuroimmune interface for dopaminergic neuroprotection/repair in Parkinson's disease.” J Mol Cell Biol 6(1):13-26 Details

L'Episcopo F, Tirolo C, Testa N, Caniglia S, Morale MC, Serapide MF, Pluchino S, Marchetti B (2014), “Wnt/?-Catenin Signaling Is Required to Rescue Midbrain Dopaminergic Progenitors and Promote Neurorepair in Ageing Mouse Model of Parkinson's Disease.” Stem Cells 32(8):2147-63 Details

Peruzzotti-Jametti L, Donegá M, Giusto E, Mallucci G, Marchetti B, Pluchino S (2014), “The role of the immune system in central nervous system plasticity after acute injury.” Neuroscience Details

Smith JA, Leonardi T, Huang B, Iraci N, Vega B, Pluchino S (2014), “Extracellular vesicles and their synthetic analogues in aging and age-associated brain diseases.” Biogerontology Details


Drago D, Cossetti C, Iraci N, Gaude E, Musco G, Bachi A, Pluchino S (2013), “The stem cell secretome and its role in brain repair.” Biochimie 95(12):2271-85 Details

Giusto E, Donegŕ M, Cossetti C, Pluchino S (2013), “Neuro-immune interactions of neural stem cell transplants: From animal disease models to human trials.” Exp Neurol Details

Hill AF, Pegtel DM, Lambertz U, Leonardi T, O'Driscoll L, Pluchino S, Ter-Ovanesyan D, Nolte-'t Hoen EN (2013), “ISEV position paper: extracellular vesicle RNA analysis and bioinformatics.” J Extracell Vesicles 2 Details

L'Episcopo F, Tirolo C, Testa N, Caniglia S, Morale MC, Impagnatiello F, Pluchino S, Marchetti B (2013), “Aging-induced Nrf2-ARE pathway disruption in the subventricular zone drives neurogenic impairment in parkinsonian mice via PI3K-Wnt/?-catenin dysregulation.” J Neurosci 33(4):1462-85 Details

Marchetti B, L'Episcopo F, Morale MC, Tirolo C, Testa N, Caniglia S, Serapide MF, Pluchino S (2013), “Uncovering novel actors in astrocyte-neuron crosstalk in Parkinson's disease: the Wnt/?-catenin signaling cascade as the common final pathway for neuroprotection and self-repair.” Eur J Neurosci 37(10):1550-63 Details

Marchetti B, Pluchino S (2013), “Wnt your brain be inflamed? Yes, it Wnt!” Trends Mol Med 19(3):144-56 Details

Pluchino S, Cossetti C (2013), “How stem cells speak with host immune cells in inflammatory brain diseases.” Glia 61(9):1379-401 Details

Pluchino S, Peruzzotti-Jametti L (2013), “Rewiring the ischaemic brain with human-induced pluripotent stem cell-derived cortical neurons.” Brain 136(Pt 12):3525-7 Details


Bolton S, Hassall D, Hildebrand D, Brück W, Huang JK, Pluchino S, McKevitt T, Parry J, McFarlane M, Fagg R, Roulois A (2012), “Anti-inflammatory Disease Therapies: Challenges and Strategies in Drug Development.” Toxicol Pathol 40(1):122-5

Cossetti C, Alfaro-Cervello C, Donegŕ M, Tyzack G, Pluchino S (2012), “New perspectives of tissue remodelling with neural stem and progenitor cell-based therapies.” Cell Tissue Res Details

Cossetti C, Smith JA, Iraci N, Leonardi T, Alfaro-Cervello C, Pluchino S (2012), “Extracellular membrane vesicles and immune regulation in the brain.” Front Physiol 3:117 Details

Herz J, Reitmeir R, Hagen SI, Reinboth BS, Guo Z, Zechariah A, Elali A, Doeppner TR, Bacigaluppi M, Pluchino S, Kilic U, Kilic E, Hermann DM (2012), “Intracerebroventricularly delivered VEGF promotes contralesional corticorubral plasticity after focal cerebral ischemia via mechanisms involving anti-inflammatory actions.” Neurobiol Dis 45(3):1077-85 Details

Kalra H, Simpson RJ, Ji H, Aikawa E, Altevogt P, Askenase P, Bond VC, Borrŕs FE, Breakefield X, Budnik V, Buzas E, Camussi G, Clayton A, Cocucci E, Falcon-Perez JM, Gabrielsson S, Gho YS, Gupta D, Harsha HC, Hendrix A, Hill AF, Inal JM, Jenster G, Krämer-Albers EM, Lim SK, Llorente A, Lötvall J, Marcilla A, Mincheva-Nilsson L, Nazarenko I, Nieuwland R, Nolte-'t Hoen EN, Pandey A, Patel T, Piper MG, Pluchino S, Prasad TS, Rajendran L, Raposo G, Record M, Reid GE, Sánchez-Madrid F, Schiffelers RM, Siljander P, Stensballe A, Stoorvogel W, Taylor D, Thery C, Valadi H, van Balkom BW, Vázquez J, Vidal M, Wauben MH, Yáńez-Mó M, Zoeller M, Mathivanan S (2012), “Vesiclepedia: a compendium for extracellular vesicles with continuous community annotation.” PLoS Biol 10(12):e1001450 Details

L'episcopo F, Tirolo C, Testa N, Caniglia S, Morale MC, Deleidi M, Serapide MF, Pluchino S, Marchetti B (2012), “Plasticity of Subventricular Zone Neuroprogenitors in MPTP (1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine) Mouse Model of Parkinson's Disease Involves Cross Talk between Inflammatory and Wnt/?-Catenin Signaling Pathways: Functional Consequences for Neuroprotection and Repair.” J Neurosci 32(6):2062-2085 Details


Cimadamore F, Fishwick K, Giusto E, Gnedeva K, Cattarossi G, Miller A, Pluchino S, Brill LM, Bronner-Fraser M, Terskikh AV (2011), “Human ESC-derived neural crest model reveals a key role for SOX2 in sensory neurogenesis.” Cell Stem Cell 8(5):538-51 Details

L'episcopo F, Serapide MF, Tirolo C, Testa N, Caniglia S, Morale MC, Pluchino S, Marchetti B (2011), “A Wnt1 regulated Frizzled-1/?-Catenin signaling pathway as a candidate regulatory circuit controlling mesencephalic dopaminergic neuron-astrocyte crosstalk: Therapeutical relevance for neuron survival and neuroprotection.” Mol Neurodegener 6:49 Details

Martino G, Pluchino S, Bonfanti L, Schwartz M (2011), “Brain regeneration in physiology and pathology: the immune signature driving therapeutic plasticity of neural stem cells.” Physiol Rev 91(4):1281-304 Details

Pluchino S, Alfaro-Cervello C (2011), “Cyclooxygenase (COX)-1 takes control of adult hippocampal neurogenesis.” Cell Cycle 10(19):3229 Details

Reitmeir R, Kilic E, Kilic U, Bacigaluppi M, ElAli A, Salani G, Pluchino S, Gassmann M, Hermann DM (2011), “Post-acute delivery of erythropoietin induces stroke recovery by promoting perilesional tissue remodelling and contralesional pyramidal tract plasticity.” Brain 134(Pt 1):84-99 Details


Freedman MS, Bar-Or A, Atkins HL, Karussis D, Frassoni F, Lazarus H, Scolding N, Slavin S, Le Blanc K, Uccelli A, MSCT Study Group (2010), “The therapeutic potential of mesenchymal stem cell transplantation as a treatment for multiple sclerosis: consensus report of the International MSCT Study Group.” Mult Scler 16(4):503-10 Details


Gagliano N, Costa F, Cossetti C, Pettinari L, Bassi R, Chiriva-Internati M, Cobos E, Gioia M, Pluchino S (2009), “Glioma-astrocyte interaction modifies the astrocyte phenotype in a co-culture experimental model.” Oncol Rep 22(6):1349-56 Details

Pluchino S, Zanotti L, Brini E, Ferrari S, Martino G (2009), “Regeneration and repair in multiple sclerosis: the role of cell transplantation.” Neurosci Lett 456(3):101-6 Details

Shechter R, London A, Varol C, Raposo C, Cusimano M, Yovel G, Rolls A, Mack M, Pluchino S, Martino G, Jung S, Schwartz M (2009), “Infiltrating blood-derived macrophages are vital cells playing an anti-inflammatory role in recovery from spinal cord injury in mice.” PLoS Med 6(7):e1000113 Details


Bacigaluppi M, Pluchino S, Martino G, Kilic E, Hermann DM (2008), “Neural stem/precursor cells for the treatment of ischemic stroke.” J Neurol Sci 265(1-2):73-7 Details

Butti E, Bergami A, Recchia A, Brambilla E, Del Carro U, Amadio S, Cattalini A, Esposito M, Stornaiuolo A, Comi G, Pluchino S, Mavilio F, Martino G, Furlan R (2008), “IL4 gene delivery to the CNS recruits regulatory T cells and induces clinical recovery in mouse models of multiple sclerosis.” Gene Ther 15(7):504-15 Details

Pluchino S, Martino G (2008), “Neural stem cell-mediated immunomodulation: repairing the haemorrhagic brain.” Brain 131(Pt 3):604-5 Details

Pluchino S, Martino G (2008), “The therapeutic plasticity of neural stem/precursor cells in multiple sclerosis.” J Neurol Sci 265(1-2):105-10 Details

Serra PA, Pluchino S, Marchetti B, Desole MS, Miele E (2008), “The MPTP mouse model: cues on DA release and neural stem cell restorative role.” Parkinsonism Relat Disord 14 Suppl 2:S189-93 Details


Martino G, Pluchino S (2007), “Neural stem cells: guardians of the brain.” Nat Cell Biol 9(9):1031-4 Details


Amadio S, Pluchino S, Brini E, Morana P, Guerriero R, Boneschi FM, Comi G, Zaratin P, Muzio V, del Carro U (2006), “Motor evoked potentials in a mouse model of chronic multiple sclerosis.” Muscle Nerve 33(2):265-73 Details

Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M (2006), “Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.” Mol Cell Neurosci 31(1):149-60 Details

Capriles E, Do Campo A, Verde O, Pluchino S, Capriles Hulett A (2006), “Children's asthma and the third world: an approach.” J Investig Allergol Clin Immunol 16(1):11-8 Details

Martino G, Pluchino S (2006), “The therapeutic potential of neural stem cells.” Nat Rev Neurosci 7(5):395-406 Details

Ziv Y, Avidan H, Pluchino S, Martino G, Schwartz M (2006), “Synergy between immune cells and adult neural stem/progenitor cells promotes functional recovery from spinal cord injury.” Proc Natl Acad Sci U S A 103(35):13174-9 Details


Pluchino S, Bacigaluppi M, Bucello S, Butti E, Deleidi M, Zanotti L, Martino G, Furlan R (2005), “Gene and stem cell therapy for autoimmune demyelination.” Ernst Schering Res Found Workshop (53):133-46 Details

Pluchino S, Martino G (2005), “The therapeutic use of stem cells for myelin repair in autoimmune demyelinating disorders.” J Neurol Sci 233(1-2):117-9 Details

Pluchino S, Zanotti L, Deleidi M, Martino G (2005), “Neural stem cells and their use as therapeutic tool in neurological disorders.” Brain Res Brain Res Rev 48(2):211-9 Details


Furlan R, Butti E, Pluchino S, Martino G (2004), “Gene therapy for autoimmune diseases.” Curr Opin Mol Ther 6(5):525-36 Details


Furlan R, Brambilla E, Sanvito F, Roccatagliata L, Olivieri S, Bergami A, Pluchino S, Uccelli A, Comi G, Martino G (2003), “Vaccination with amyloid-beta peptide induces autoimmune encephalomyelitis in C57/BL6 mice.” Brain 126(Pt 2):285-91 Details

Furlan R, Pluchino S, Marconi PC, Martino G (2003), “Cytokine gene delivery into the central nervous system using intrathecally injected nonreplicative viral vectors.” Methods Mol Biol 215:279-89 Details

Furlan R, Pluchino S, Martino G (2003), “Gene therapy-mediated modulation of immune processes in the central nervous system.” Curr Pharm Des 9(24):2002-8 Details

Furlan R, Pluchino S, Martino G (2003), “The therapeutic use of gene therapy in inflammatory demyelinating diseases of the central nervous system.” Curr Opin Neurol 16(3):385-92 Details

Hernández Muńoz G, Pluchino S (2003), “Cimicifuga racemosa for the treatment of hot flushes in women surviving breast cancer.” Maturitas 44 Suppl 1:S59-65 Details

Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G (2003), “Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis.” Nature 422(6933):688-94 Details

Pluchino S, Zanotti L, Martino G (2003), “Antibodies and myelination: facts and misacts.” Neurol Sci 24 Suppl 4:S231-3 Details


Annunziata P, Pluchino S, Martino T, Guazzi G (1997), “High levels of cerebrospinal fluid IgM binding to myelin basic protein are associated with early benign course in multiple sclerosis.” J Neuroimmunol 77(1):128-33 Details