Dr Matthias Landgraf
Dr Matthias Landgraf is pleased to consider applications from prospective PhD students.
http://www.zoo.cam.ac.uk/departm... (personal home page)
Development of neural networks - from the morphogenesis of dendritic trees to patterns of connections.
As neuronal circuits form, synaptic terminals are delivered to specific regions of the nervous system so that connections can form between appropriate partner neurons. We investigate the genetic and cellular mechanisms that underlie this process. To this end we have generated a battery of genetic tools that allow us to differentially manipulate, visualise and image identified partner neurons as the network forms and develops. Using these methods we apply genetics to study the mechanisms that regulate the targeting, growth and branching of synaptic terminal arbors, and how this interlinks with the formation and adjustment of synapses.
Linking our work to the clinically relevant, we find that reactive oxygen species, which are elevated with ageing and in neurodegenerative conditions, are part of elementary signalling pathways that regulates synaptic terminal growth and adjustment.
Electrophysiological recording techniques
Genetic intersectional strategies
Associated News Items
Oswald MCW, Brooks PS, Zwart MF, Mukherjee A, West RJH, Morarach K, Sweeney ST and Landgraf M. (in submission), “Reactive Oxygen Species Regulate Activity-Dependent Neuronal Structural Plasticity. ” bioRxiv DOI: dx.doi.org/10.1101/081968
Zwart MF, Pulver SR, Truman JW, Fushiki A, Fetter, RD, Cardona A, Landgraf M. (2016), “Selective Inhibition Mediates the Sequential Recruitment of Motor Pools. ” Neuron 91(3):615-628
Couton L, Mauss AS, Yunusov T, Diegelmann S, Evers JF, Landgraf M. (2015), “Development of connectivity in a motoneuronal network in Drosophila larvae. ” Current Biology 25(5): 568–576
Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, Potter CJ, Landgraf M, White BH. (2015), “Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes. ” Cell Reports 10(8): 1410–1421
Zwart MF, Randlett O, Evers JF, Landgraf M (2013), “Dendritic growth gated by a steroid hormone receptor underlies increases in activity in the developing Drosophila locomotor system.” Proc Natl Acad Sci U S A 110(40):E3878-87 Details
Singh AP, Das RN, Rao G, Aggarwal A, Diegelmann S, Evers JF, Karandikar H, Landgraf M, Rodrigues V, Vijayraghavan K (2013), “Sensory neuron-derived eph regulates glomerular arbors and modulatory function of a central serotonergic neuron.” PLoS Genet 9(4):e1003452 Details
Thackray AM, Muhammad F, Zhang C, Di Y, Jahn TR, Landgraf M, Crowther DC, Evers JF, Bujdoso R (2012), “Ovine PrP transgenic Drosophila show reduced locomotor activity and decreased survival.” Biochem J 444(3):487-95 Details
Nicolaï LJ, Ramaekers A, Raemaekers T, Drozdzecki A, Mauss AS, Yan J, Landgraf M, Annaert W, Hassan BA (2010), “Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila.” Proc Natl Acad Sci U S A 107(47):20553-8 Details
Mauss A, Tripodi M, Evers JF, Landgraf M (2009), “Midline signalling systems direct the formation of a neural map by dendritic targeting in the Drosophila motor system.” PLoS Biol 7(9):e1000200 Details
Diegelmann S, Bate M, Landgraf M (2008), “Gateway cloning vectors for the LexA-based binary expression system in Drosophila.” Fly (Austin) 2(4):236-9 Details
Ou Y, Chwalla B, Landgraf M, van Meyel DJ (2008), “Identification of genes influencing dendrite morphogenesis in developing peripheral sensory and central motor neurons.” Neural Dev 3:16 Details
Tripodi M, Evers JF, Mauss A, Bate M, Landgraf M (2008), “Structural homeostasis: compensatory adjustments of dendritic arbor geometry in response to variations of synaptic input.” PLoS Biol 6(10):e260 Details
Landgraf M, Thor S (2006), “Development and structure of motoneurons.” Int Rev Neurobiol 75:33-53 Details
Landgraf M, Thor S (2006), “Development of Drosophila motoneurons: specification and morphology.” Semin Cell Dev Biol 17(1):3-11 Details
Landgraf M, Evers JF (2005), “Control of dendritic diversity.” Curr Opin Cell Biol 17(6):690-6 Details
Landgraf M, Jeffrey V, Fujioka M, Jaynes JB, Bate M (2003), “Embryonic origins of a motor system: motor dendrites form a myotopic map in Drosophila.” PLoS Biol 1(2):E41 Details
Landgraf M, Sánchez-Soriano N, Technau GM, Urban J, Prokop A (2003), “Charting the Drosophila neuropile: a strategy for the standardised characterisation of genetically amenable neurites.” Dev Biol 260(1):207-25 Details
Zlatic M, Landgraf M, Bate M (2003), “Genetic specification of axonal arbors: atonal regulates robo3 to position terminal branches in the Drosophila nervous system.” Neuron 37(1):41-51 Details