Dr John O'Neill

John O'Neill

University position

Group Leader

Dr John O'Neill is pleased to consider applications from prospective PhD students.


Medical Research Council Laboratory of Molecular Biology

Home page


Research Theme

Cellular and Molecular Neuroscience


The causes and consequences of cellular circadian timekeeping and its interaction with sleep.

Research Focus


Circadian Rhythm

Metabolic Oscillation

Biological Clock


Clinical conditions

No direct clinical relevance


Calcium imaging

Cell culture

Enzyme assays

Field potential recording

Long-term bioluminescence assays


Protein purification

Recombinant protein expression



Michael Hastings

Akhilesh Reddy

Associated News Items



    Hoyle NP, O'Neill JS (2015), “Oxidation-reduction cycles of peroxiredoxin proteins and nontranscriptional aspects of timekeeping.” Biochemistry 54(2):184-93 Details

    Milev NB, Rey G, Valekunja UK, Edgar RS, O'Neill JS, Reddy AB (2015), “Analysis of the redox oscillations in the circadian clockwork.” Methods Enzymol 552:185-210 Details


    Fogg PC, O'Neill JS, Dobrzycki T, Calvert S, Lord EC, McIntosh RL, Elliott CJ, Sweeney ST, Hastings MH, Chawla S (2014), “Class IIa histone deacetylases are conserved regulators of circadian function.” J Biol Chem 289(49):34341-8 Details

    O'Neill JS, Feeney KA (2014), “Circadian redox and metabolic oscillations in mammalian systems.” Antioxid Redox Signal 20(18):2966-81 Details


    Hoyle NP, O'Neill JS (2013), “Circadian rhythms: hijacking the cyanobacterial clock.” Curr Biol 23(23):R1050-2 Details

    Maywood ES, Drynan L, Chesham JE, Edwards MD, Dardente H, Fustin JM, Hazlerigg DG, O'Neill JS, Codner GF, Smyllie NJ, Brancaccio M, Hastings MH (2013), “Analysis of core circadian feedback loop in suprachiasmatic nucleus of mCry1-luc transgenic reporter mouse.” Proc Natl Acad Sci U S A 110(23):9547-52 Details

    O'Neill JS, Maywood ES, Hastings MH (2013), “Cellular mechanisms of circadian pacemaking: beyond transcriptional loops.” Handb Exp Pharmacol (217):67-103 Details

    Valekunja UK, Edgar RS, Oklejewicz M, van der Horst GT, O'Neill JS, Tamanini F, Turner DJ, Reddy AB (2013), “Histone methyltransferase MLL3 contributes to genome-scale circadian transcription.” Proc Natl Acad Sci U S A 110(4):1554-9 Details

    van Ooijen G, Hindle M, Martin SF, Barrios-Llerena M, Sanchez F, Bouget FY, O'Neill JS, Le Bihan T, Millar AJ (2013), “Functional analysis of Casein Kinase 1 in a minimal circadian system.” PLoS One 8(7):e70021 Details

    van Ooijen G, Martin SF, Barrios-Llerena ME, Hindle M, Le Bihan T, O'Neill JS, Millar AJ (2013), “Functional analysis of the rodent CK1tau mutation in the circadian clock of a marine unicellular alga.” BMC Cell Biol 14:46 Details


    Edgar RS, Green EW, Zhao Y, van Ooijen G, Olmedo M, Qin X, Xu Y, Pan M, Valekunja UK, Feeney KA, Maywood ES, Hastings MH, Baliga NS, Merrow M, Millar AJ, Johnson CH, Kyriacou CP, O'Neill JS, Reddy AB (2012), “Peroxiredoxins are conserved markers of circadian rhythms.” Nature 485(7399):459-64 Details

    O'Neill JS, Reddy AB (2012), “The essential role of cAMP/Ca2+ signalling in mammalian circadian timekeeping.” Biochem Soc Trans 40(1):44-50 Details

    Olmedo M, O'Neill JS, Edgar RS, Valekunja UK, Reddy AB, Merrow M (2012), “Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans.” Proc Natl Acad Sci U S A 109(50):20479-84 Details


    Le Bihan T, Martin SF, Chirnside ES, van Ooijen G, Barrios-Llerena ME, O'Neill JS, Shliaha PV, Kerr LE, Millar AJ (2011), “Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri.” J Proteomics 74(10):2060-70 Details

    O'Neill JS, Reddy AB (2011), “Circadian clocks in human red blood cells.” Nature 469(7331):498-503 Details

    O'Neill JS, van Ooijen G, Dixon LE, Troein C, Corellou F, Bouget FY, Reddy AB, Millar AJ (2011), “Circadian rhythms persist without transcription in a eukaryote.” Nature 469(7331):554-8 Details

    O'Neill JS, van Ooijen G, Le Bihan T, Millar AJ (2011), “Circadian clock parameter measurement: characterization of clock transcription factors using surface plasmon resonance.” J Biol Rhythms 26(2):91-8 Details

    Reddy AB, O'Neill JS (2011), “Metaclocks.” EMBO Rep 12(7):612 Details

    Troein C, Corellou F, Dixon LE, van Ooijen G, O'Neill JS, Bouget FY, Millar AJ (2011), “Multiple light inputs to a simple clock circuit allow complex biological rhythms.” Plant J 66(2):375-85 Details


    Reddy AB, O'Neill JS (2010), “Healthy clocks, healthy body, healthy mind.” Trends Cell Biol 20(1):36-44 Details


    Fustin JM, O'Neill JS, Hastings MH, Hazlerigg DG, Dardente H (2009), “Cry1 circadian phase in vitro: wrapped up with an E-box.” J Biol Rhythms 24(1):16-24 Details

    O'Neill JS (2009), “Circadian clocks can take a few transcriptional knocks.” EMBO J 28(2):84-5 Details


    Hastings MH, Maywood ES, O'Neill JS (2008), “Cellular circadian pacemaking and the role of cytosolic rhythms.” Curr Biol 18(17):R805-R815 Details

    O'Neill JS, Hastings MH (2008), “Increased coherence of circadian rhythms in mature fibroblast cultures.” J Biol Rhythms 23(6):483-8 Details

    O'Neill JS, Maywood ES, Chesham JE, Takahashi JS, Hastings MH (2008), “cAMP-dependent signaling as a core component of the mammalian circadian pacemaker.” Science 320(5878):949-53 Details


    Godinho SI, Maywood ES, Shaw L, Tucci V, Barnard AR, Busino L, Pagano M, Kendall R, Quwailid MM, Romero MR, O'neill J, Chesham JE, Brooker D, Lalanne Z, Hastings MH, Nolan PM (2007), “The after-hours mutant reveals a role for Fbxl3 in determining mammalian circadian period.” Science 316(5826):897-900 Details

    Hastings M, O'Neill JS, Maywood ES (2007), “Circadian clocks: regulators of endocrine and metabolic rhythms.” J Endocrinol 195(2):187-98 Details

    Lewis AM, Masgrau R, Vasudevan SR, Yamasaki M, O'Neill JS, Garnham C, James K, Macdonald A, Ziegler M, Galione A, Churchill GC (2007), “Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate.” Anal Biochem 371(1):26-36 Details

    Maywood ES, O'Neill JS, Chesham JE, Hastings MH (2007), “Minireview: The circadian clockwork of the suprachiasmatic nuclei--analysis of a cellular oscillator that drives endocrine rhythms.” Endocrinology 148(12):5624-34 Details

    Maywood ES, O'Neill JS, Reddy AB, Chesham JE, Prosser HM, Kyriacou CP, Godinho SI, Nolan PM, Hastings MH (2007), “Genetic and molecular analysis of the central and peripheral circadian clockwork of mice.” Cold Spring Harb Symp Quant Biol 72:85-94 Details

    O'Neill JS, Hastings MH (2007), “Circadian clocks: timely interference by microRNAs.” Curr Biol 17(17):R760-2 Details

    Sládek M, Rybová M, Jindráková Z, Zemanová Z, Polidarová L, Mrnka L, O'Neill J, Pácha J, Sumová A (2007), “Insight into the circadian clock within rat colonic epithelial cells.” Gastroenterology 133(4):1240-9 Details


    Maywood ES, O'Neill J, Wong GK, Reddy AB, Hastings MH (2006), “Circadian timing in health and disease.” Prog Brain Res 153:253-69 Details

    Maywood ES, Reddy AB, Wong GK, O'Neill JS, O'Brien JA, McMahon DG, Harmar AJ, Okamura H, Hastings MH (2006), “Synchronization and maintenance of timekeeping in suprachiasmatic circadian clock cells by neuropeptidergic signaling.” Curr Biol 16(6):599-605 Details

    Reddy AB, Karp NA, Maywood ES, Sage EA, Deery M, O'Neill JS, Wong GK, Chesham J, Odell M, Lilley KS, Kyriacou CP, Hastings MH (2006), “Circadian orchestration of the hepatic proteome.” Curr Biol 16(11):1107-15 Details


    Reddy AB, Wong GK, O'Neill J, Maywood ES, Hastings MH (2005), “Circadian clocks: neural and peripheral pacemakers that impact upon the cell division cycle.” Mutat Res 574(1-2):76-91 Details


    Churchill GC, O'Neill JS, Masgrau R, Patel S, Thomas JM, Genazzani AA, Galione A (2003), “Sperm deliver a new second messenger: NAADP.” Curr Biol 13(2):125-8 Details