Dr Howard Baylis University positionReader and Deputy Head of Department Dr Howard Baylis is pleased to consider applications from prospective PhD students. DepartmentsHome pagehttp://www.zoo.cam.ac.uk/directo... (personal home page) Research ThemeInterestsWe are investigating the functions of genes involved in Alzheimer’s disease using C. elegans. We have focussed on presenilin genes as presenilin mutations in human cause familial Alzheimer’s disease. We aim to address the mechanism by which presenilin mutations alter neuronal function by dissecting their role at the synapse and investigating their interactions with inositol 1,4,5-trisphosphate (IP3) signalling. We also study other aspects of IP3 signalling in the nervous system including studies on the role of IP3 signalling in polymodal sensory neurones. Research Focus
EquipmentBehavioural analysis Calcium imaging Confocal microscopy Fluorescence microscopy Microscopy Collaborators
Associated News ItemsKey publicationsBaylis HA (2010), “Linking presenilins to synaptic function and calcium signaling in C. elegans” Alzheimer's & Dementia 6: S61 Walker DS, Vázquez-Manrique RP, Gower NJ, Gregory E, Schafer WR, Baylis HA (2009), “Inositol 1,4,5-trisphosphate signalling regulates the avoidance response to nose touch in Caenorhabditis elegans.” PLoS Genet 5(9):e1000636 Details Publications2015Kowalski MP, Baylis HA, Krude T (2015), “Non-coding stem-bulge RNAs are required for cell proliferation and embryonic development in C. elegans” J Cell Sci. 128: 2118-2129 Nagy AI, Vázquez-Manrique RP, Lopez M, Christov C, Sequedo MD, Herzog M, Herlihy AE, Bodak M, Gatsi R, Baylis HA (2015), “IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans” EMBO Rep. 16: 341-350 2013Favrin G, Bean DM, Bilsland E, Boyer H, Fischer BE, Russell S, Crowther DC, Baylis HA, Oliver SG, Giannakou ME (2013), “Identification of novel modifiers of A? toxicity by transcriptomic analysis in the fruitfly.” Sci Rep 3:3512 Details 2012Baylis HA, Vázquez-Manrique RP (2012), “Genetic analysis of IP3 and calcium signalling pathways in C. elegans.” Biochim Biophys Acta 1820(8):1253-68 Details 2011Baylis HA, Vázquez-Manrique RP (2011), “Reverse genetic strategies in Caenorhabditis elegans: towards controlled manipulation of the genome.” ScientificWorldJournal 11:1394-410 Details in pressGonzalez-Cabo P, Bolinches-Amorós A, Cabello J, Ros S, Moreno S, Baylis H A, Palau F and Vazquez-Manrique R P (in press), “Disruption of the ATP-binding cassette B7 (ABTM-1/ABCB7) induces oxidative stress and premature cell death in Caenorhabditis elegans. ” J Biol Chem 2011González-Cabo P, Bolinches-Amorós A, Cabello J, Ros S, Moreno S, Baylis HA, Palau F, Vázquez-Manrique RP (2011), “Disruption of the ATP-binding cassette B7 (ABTM-1/ABCB7) induces oxidative stress and premature cell death in Caenorhabditis elegans.” J Biol Chem 286(24):21304-14 Details 2010Vázquez-Manrique RP, Legg JC, Olofsson B, Ly S, Baylis HA (2010), “Improved gene targeting in C. elegans using counter-selection and Flp-mediated marker excision.” Genomics 95(1):37-46 Details 2009Parker S, Baylis HA (2009), “Overexpression of caveolins in Caenorhabditis elegans induces changes in egg-laying and fecundity.” Commun Integr Biol 2(5):382-4 Details Parker S, Walker DS, Ly S, Baylis HA (2009), “Caveolin-2 is required for apical lipid trafficking and suppresses basolateral recycling defects in the intestine of Caenorhabditis elegans.” Mol Biol Cell 20(6):1763-71 Details 2008Kwan CS, Vázquez-Manrique RP, Ly S, Goyal K, Baylis HA (2008), “TRPM channels are required for rhythmicity in the ultradian defecation rhythm of C. elegans.” BMC Physiol 8:11 Details Vázquez-Manrique RP, Nagy AI, Legg JC, Bales OA, Ly S, Baylis HA (2008), “Phospholipase C-epsilon regulates epidermal morphogenesis in Caenorhabditis elegans.” PLoS Genet 4(3):e1000043 Details 2007Baylis HA, Goyal K (2007), “TRPM channel function in Caenorhabditis elegans.” Biochem Soc Trans 35(Pt 1):129-32 Details Parker S, Peterkin HS, Baylis HA (2007), “Muscular dystrophy associated mutations in caveolin-1 induce neurotransmission and locomotion defects in Caenorhabditis elegans.” Invert Neurosci 7(3):157-64 Details Vázquez-Manrique RP, González-Cabo P, Ortiz-Martín I, Ros S, Baylis HA, Palau F (2007), “The frataxin-encoding operon of Caenorhabditis elegans shows complex structure and regulation.” Genomics 89(3):392-401 Details 2006Vázquez-Manrique RP, González-Cabo P, Ros S, Aziz H, Baylis HA, Palau F (2006), “Reduction of Caenorhabditis elegans frataxin increases sensitivity to oxidative stress, reduces lifespan, and causes lethality in a mitochondrial complex II mutant.” FASEB J 20(1):172-4 Details 2005Baylis HA (2005), “VAV's got rhythm.” Cell 123(1):5-7 Details Gower NJ, Walker DS, Baylis HA (2005), “Inositol 1,4,5-trisphosphate signaling regulates mating behavior in Caenorhabditis elegans males.” Mol Biol Cell 16(9):3978-86 Details 2004Culetto E, Baylis HA, Richmond JE, Jones AK, Fleming JT, Squire MD, Lewis JA, Sattelle DB (2004), “The Caenorhabditis elegans unc-63 gene encodes a levamisole-sensitive nicotinic acetylcholine receptor alpha subunit.” J Biol Chem 279(41):42476-83 Details Culletto E, Baylis HA, Richmond JE, Jones AK, Fleming JT, Squire MD, Lewis JA, Sattelle DB (2004), “The Caenorhabditis elegans unc-63 gene encodes a levamisole-sensitive nicotinic acetylcholine receptor alpha subunit” J Biol Chem 279:42476-42483 Walker DS, Ly S, Gower NJ, Baylis HA (2004), “IRI-1, a LIN-15B homologue, interacts with inositol-1,4,5-triphosphate receptors and regulates gonadogenesis, defecation, and pharyngeal pumping in Caenorhabditis elegans.” Mol Biol Cell 15(7):3073-82 Details Yin X, Gower NJ, Baylis HA, Strange K (2004), “Inositol 1,4,5-trisphosphate signaling regulates rhythmic contractile activity of myoepithelial sheath cells in Caenorhabditis elegans.” Mol Biol Cell 15(8):3938-49 Details 2002Walker DS, Gower NJ, Ly S, Bradley GL, Baylis HA (2002), “Regulated disruption of inositol 1,4,5-trisphosphate signaling in Caenorhabditis elegans reveals new functions in feeding and embryogenesis.” Mol Biol Cell 13(4):1329-37 Details Walker DS, Gower NJD, Ly S, Bradley GL, Baylis HA (2002), “Regulated disruption of inositol 1,4,5-trisphosphate (IP3) signaling in C, elegans reveals new functions in feeding and embryogenesis” Mol Biol Cell 13:1329-1337 Walker DS, Ly S, Lockwood KC, Baylis HA (2002), “A direct interaction between IP(3) receptors and myosin II regulates IP(3) signaling in C. elegans.” Curr Biol 12(11):951-6 Details 2001Gower NJ, Temple GR, Schein JE, Marra M, Walker DS, Baylis HA (2001), “Dissection of the promoter region of the inositol 1,4,5-trisphosphate receptor gene, itr-1, in C. elegans: a molecular basis for cell-specific expression of IP3R isoforms.” J Mol Biol 306(2):145-57 Details 1999Baylis HA, Furuichi T, Yoshikawa F, Mikoshiba K, Sattelle DB (1999), “Inositol 1,4,5-trisphosphate receptors are strongly expressed in the nervous system, pharynx, intestine, gonad and excretory cell of Caenorhabditis elegans and are encoded by a single gene (itr-1).” J Mol Biol 294(2):467-76 Details Miguel-Aliaga I, Culetto E, Walker DS, Baylis HA, Sattelle DB, Davies KE (1999), “The Caenorhabditis elegans orthologue of the human gene responsible for spinal muscular atrophy is a maternal product critical for germline maturation and embryonic viability.” Hum Mol Genet 8(12):2133-43 Details 1998Buckingham SD, Adcock C, Sansom MS, Sattelle DB, Baylis HA (1998), “Functional characterization of a mutated chicken alpha7 nicotinic acetylcholine receptor subunit with a leucine residue inserted in transmembrane domain 2.” Br J Pharmacol 124(4):747-55 Details Matsuda K, Buckingham SD, Freeman JC, Squire MD, Baylis HA, Sattelle DB (1998), “Effects of the alpha subunit on imidacloprid sensitivity of recombinant nicotinic acetylcholine receptors.” Br J Pharmacol 123(3):518-24 Details Mongan NP, Baylis HA, Adcock C, Smith GR, Sansom MS, Sattelle DB (1998), “An extensive and diverse gene family of nicotinic acetylcholine receptor alpha subunits in Caenorhabditis elegans.” Receptors Channels 6(3):213-28 Details 1997Baylis HA, Matsuda K, Squire MD, Fleming JT, Harvey RJ, Darlison MG, Barnard EA, Sattelle DB (1997), “ACR-3, a Caenorhabditis elegans nicotinic acetylcholine receptor subunit. Molecular cloning and functional expression.” Receptors Channels 5(3-4):149-58 Details Rauh JJ, Holyoke CW, Kleier DA, Presnail JK, Benner EA, Cordova D, Howard MH, Hosie AM, Buckingham SD, Baylis HA, Sattelle DB (1997), “Polycyclic dinitriles: a novel class of potent GABAergic insecticides provides a new radioligand, [3H]BIDN.” Invert Neurosci 3(2-3):261-8 Details 1996Baylis HA, Sattelle DB, Lane NJ (1996), “Genetic analysis of cholinergic nerve terminal function in invertebrates.” J Neurocytol 25(12):747-62 Details Buckingham SD, Matsuda K, Hosie AM, Baylis HA, Squire MD, Lansdell SJ, Millar NS, Sattelle B (1996), “Wild-type and insecticide-resistant homo-oligomeric GABA receptors of Drosophila melanogaster stably expressed in a Drosophila cell line.” Neuropharmacology 35(9-10):1393-401 Details Fleming JT, Baylis HA, Sattelle DB, Lewis JA (1996), “Molecular cloning and in vitro expression of C. elegans and parasitic nematode ionotropic receptors.” Parasitology 113 Suppl:S175-90 Details Matsuda K, Hosie AM, Buckingham SD, Squire MD, Baylis HA, Sattelle DB (1996), “pH-dependent actions of THIP and ZAPA on an ionotropic Drosophila melanogaster GABA receptor.” Brain Res 739(1-2):335-8 Details 1995Baylis HA, Megson A, Hall R (1995), “Infection with Theileria annulata induces expression of matrix metalloproteinase 9 and transcription factor AP-1 in bovine leucocytes.” Mol Biochem Parasitol 69(2):211-22 Details Hosie AM, Baylis HA, Buckingham SD, Sattelle DB (1995), “Actions of the insecticide fipronil, on dieldrin-sensitive and- resistant GABA receptors of Drosophila melanogaster.” Br J Pharmacol 115(6):909-12 Details Hosie AM, Shirai Y, Buckingham SD, Rauh JJ, Roush RT, Baylis HA, Sattelle DB (1995), “Blocking actions of BIDN, a bicyclic dinitrile convulsant compound, on wild-type and dieldrin-resistant GABA receptor homo-oligomers of Drosophila melanogaster expressed in Xenopus oocytes.” Brain Res 693(1-2):257-60 Details Millar NS, Baylis HA, Reaper C, Bunting R, Mason WT, Sattelle DB (1995), “Functional expression of a cloned Drosophila muscarinic acetylcholine receptor in a stable Drosophila cell line.” J Exp Biol 198(Pt 9):1843-50 Details Shirai Y, Hosie AM, Buckingham SD, Holyoke CW Jr, Baylis HA, Sattelle DB (1995), “Actions of picrotoxinin analogues on an expressed, homo-oligomeric GABA receptor of Drosophila melanogaster.” Neurosci Lett 189(1):1-4 Details Squire MD, Tornře C, Baylis HA, Fleming JT, Barnard EA, Sattelle DB (1995), “Molecular cloning and functional co-expression of a Caenorhabditis elegans nicotinic acetylcholine receptor subunit (acr-2).” Receptors Channels 3(2):107-15 Details 1994Boulter N, Knight PA, Hunt PD, Hennessey ES, Katzer F, Tait A, Williamson S, Brown D, Baylis HA, Hall R (1994), “Theileria annulata sporozoite surface antigen (SPAG-1) contains neutralizing determinants in the C terminus.” Parasite Immunol 16(2):97-104 Details 1993Allsopp BA, Baylis HA, Allsopp MT, Cavalier-Smith T, Bishop RP, Carrington DM, Sohanpal B, Spooner P (1993), “Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences.” Parasitology 107 ( Pt 2):157-65 Details Baylis HA, Allsopp BA, Hall R, Carrington M (1993), “Characterisation of a glutamine- and proline-rich protein (QP protein) from Theileria parva.” Mol Biochem Parasitol 61(2):171-8 Details 1992Baylis HA, Megson A, Brown CG, Wilkie GF, Hall R (1992), “Theileria annulata-infected cells produce abundant proteases whose activity is reduced by long-term cell culture.” Parasitology 105 ( Pt 3):417-23 Details Baylis HA, Megson A, Mottram JC, Hall R (1992), “Characterisation of a gene for a cysteine protease from Theileria annulata.” Mol Biochem Parasitol 54(1):105-7 Details 1991Baylis HA, Sohal SK, Carrington M, Bishop RP, Allsopp BA (1991), “An unusual repetitive gene family in Theileria parva which is stage-specifically transcribed.” Mol Biochem Parasitol 49(1):133-42 Details Megson A, Inman GJ, Hunt PD, Baylis HA, Hall R (1991), “The gene for apocytochrome B of Theileria annulata resides on a small linear extrachromosomal element.” Mol Biochem Parasitol 48(1):113-5 Details Strauch E, Takano E, Baylis HA, Bibb MJ (1991), “The stringent response in Streptomyces coelicolor A3(2).” Mol Microbiol 5(2):289-98 Details 1988Baylis HA, Bibb MJ (1988), “Transcriptional analysis of the 16S rRNA gene of the rrnD gene set of Streptomyces coelicolor A3(2).” Mol Microbiol 2(5):569-79 Details Baylis HA, Bibb MJ (1988), “Organisation of the ribosomal RNA genes in Streptomyces coelicolor A3(2).” Mol Gen Genet 211(2):191-6 Details 1987Baylis HA, Bibb MJ (1987), “The nucleotide sequence of a 16S rRNA gene from Streptomyces coelicolor A3(2)” Nucleic Acids Res 15(17):7176 Details Lawlor EJ, Baylis HA, Chater KF (1987), “Pleiotropic morphological and antibiotic deficiencies result from mutations in a gene encoding a tRNA-like product in Streptomyces coelicolor A3(2).” Genes Dev 1(10):1305-10 Details |