Dr Gregory Jefferis University positionMRC Programme Leader Dr Gregory Jefferis is pleased to consider applications from prospective PhD students. InstitutesMedical Research Council Laboratory of Molecular Biology Home pagehttp://www2.mrc-lmb.cam.ac.uk/group-l... (personal home page) Research ThemesInterestsOur broad goal is to understand how smell turns into behaviour in the fruit fly brain. We use a combination of genetic labelling and manipulation, targeted in vivo whole cell patch clamp recording and high resolution neuroanatomy to study olfactory circuits. Key interests include understanding how third order olfactory neurons of the lateral horn integrate information from specific olfactory channels. This process of selective integration is the fundamental unit of perception and is likely to underlie innate behavioural responses to general odours and sex pheromones. Please get in touch if you would like to find out more about the group.  Confocal image of fruit fly brain with GFP labelled olfactory projection neurons. On the left a single neuron has been genetically labelled. On the right a clonally related group of 30 cells derived from a single neuroblast (neural stem cell). View image full-size (5764x4022 pixels) Research Focus
EquipmentConfocal microscopy Electrophysiological recording techniques Fluorescence microscopy Genetic Labelling and Manipulation of Neurons Image Registration (for 3D Brain Atlases) Immunohistochemistry Intracellular recording Microscopy Multiphoton imaging Optogenetics Single Cell Labelling (MARCM) Whole cell patch clamp Collaborators
Associated News Items
Key publicationsKohl J, Ng J, Cachero S, Ciabatti E, Dolan MJ, Sutcliffe B, Tozer A, Ruehle S, Krueger D, Frechter S, Branco T, Tripodi M, Jefferis GS (2014), “Ultrafast tissue staining with chemical tags.” Proc Natl Acad Sci U S A 111(36):E3805-14 PDF Details Kohl J, Ostrovsky AD, Frechter S, Jefferis GS (2013), “A bidirectional circuit switch reroutes pheromone signals in male and female brains.” Cell 155(7):1610-23 PDF Details Grosjean Y, Rytz R, Farine JP, Abuin L, Cortot J, Jefferis GS, Benton R (2011), “An olfactory receptor for food-derived odours promotes male courtship in Drosophila.” Nature 478(7368):236-40 Details Cachero S, Ostrovsky AD, Yu JY, Dickson BJ, Jefferis GS (2010), “Sexual dimorphism in the fly brain.” Curr Biol 20(18):1589-601 PDF Details Yu JY, Kanai MI, Demir E, Jefferis GS, Dickson BJ (2010), “Cellular organization of the neural circuit that drives Drosophila courtship behavior.” Curr Biol 20(18):1602-14 Details Masse NY, Turner GC, Jefferis GS (2009), “Olfactory information processing in Drosophila.” Curr Biol 19(16):R700-13 Details Jefferis GS, Potter CJ, Chan AM, Marin EC, Rohlfing T, Maurer CR Jr, Luo L (2007), “Comprehensive maps of Drosophila higher olfactory centers: spatially segregated fruit and pheromone representation.” Cell 128(6):1187-203 PDF Details Jefferis GS, Marin EC, Stocker RF, Luo L (2001), “Target neuron prespecification in the olfactory map of Drosophila.” Nature 414(6860):204-8 Details Publications2015Kohl J, Huoviala P, Jefferis GS (2015), “Pheromone processing in Drosophila.” Curr Opin Neurobiol 34:149-157 Details 2012Jefferis GS, Livet J (2012), “Sparse and combinatorial neuron labelling.” Curr Opin Neurobiol 22(1):101-10 Details Masse NY, Cachero S, Ostrovsky AD, Jefferis GS (2012), “A mutual information approach to automate identification of neuronal clusters in Drosophila brain images.” Front Neuroinform 6:21 Details Osumi-Sutherland D, Reeve S, Mungall CJ, Neuhaus F, Ruttenberg A, Jefferis GS, Armstrong JD (2012), “A strategy for building neuroanatomy ontologies.” Bioinformatics 28(9):1262-9 Details 2011Brown KM, Barrionuevo G, Canty AJ, De Paola V, Hirsch JA, Jefferis GS, Lu J, Snippe M, Sugihara I, Ascoli GA (2011), “The DIADEM data sets: representative light microscopy images of neuronal morphology to advance automation of digital reconstructions.” Neuroinformatics 9(2-3):143-57 Details Cachero S, Jefferis GS (2011), “Double brainbow.” Nat Methods 8(3):217-8 Details Kohl J, Jefferis GS (2011), “Neuroanatomy: decoding the fly brain.” Curr Biol 21(1):R19-20 Details Silbering AF, Rytz R, Grosjean Y, Abuin L, Ramdya P, Jefferis GS, Benton R (2011), “Complementary function and integrated wiring of the evolutionarily distinct Drosophila olfactory subsystems.” J Neurosci 31(38):13357-75 Details 2008Cachero S, Jefferis GS (2008), “Drosophila olfaction: the end of stereotypy?” Neuron 59(6):843-5 Details 2006Antolin S, Jefferis GS (2006), “Olfactory coding: when smells collide.” Curr Biol 16(23):R1000-3 Details Jefferis GS (2006), “Wiring specificity: axon-dendrite matching refines the olfactory map.” Curr Biol 16(10):R373-6 Details Jefferis GS, Hummel T (2006), “Wiring specificity in the olfactory system.” Semin Cell Dev Biol 17(1):50-65 Details 2005Jefferis GS (2005), “Insect olfaction: a map of smell in the brain.” Curr Biol 15(17):R668-70 Details Jefferis GS, Marin EC, Komiyama T, Zhu H, Chihara T, Berdnik D, Luo L (2005), “Development of wiring specificity of the Drosophila olfactory system.” Chem Senses 30 Suppl 1:i94 Details Ramaekers A, Magnenat E, Marin EC, Gendre N, Jefferis GS, Luo L, Stocker RF (2005), “Glomerular maps without cellular redundancy at successive levels of the Drosophila larval olfactory circuit.” Curr Biol 15(11):982-92 Details Taylor Tavares AL, Jefferis GS, Koop M, Hill BC, Hastie T, Heit G, Bronte-Stewart HM (2005), “Quantitative measurements of alternating finger tapping in Parkinson's disease correlate with UPDRS motor disability and reveal the improvement in fine motor control from medication and deep brain stimulation.” Mov Disord 20(10):1286-98 Details 2004Jefferis GS, Komiyama T, Luo L (2004), “Neuroscience. Calcium and CREST for healthy dendrites.” Science 303(5655):179-81 Details Jefferis GS, Vyas RM, Berdnik D, Ramaekers A, Stocker RF, Tanaka NK, Ito K, Luo L (2004), “Developmental origin of wiring specificity in the olfactory system of Drosophila.” Development 131(1):117-30 Details 2003Komiyama T, Johnson WA, Luo L, Jefferis GS (2003), “From lineage to wiring specificity. POU domain transcription factors control precise connections of Drosophila olfactory projection neurons.” Cell 112(2):157-67 Details 2002Jefferis GS, Marin EC, Watts RJ, Luo L (2002), “Development of neuronal connectivity in Drosophila antennal lobes and mushroom bodies.” Curr Opin Neurobiol 12(1):80-6 Details Marin EC, Jefferis GS, Komiyama T, Zhu H, Luo L (2002), “Representation of the glomerular olfactory map in the Drosophila brain.” Cell 109(2):243-55 Details 2000Kuwabara PE, Lee MH, Schedl T, Jefferis GS (2000), “A C. elegans patched gene, ptc-1, functions in germ-line cytokinesis.” Genes Dev 14(15):1933-44 Details |
