Dr Eva Kaulich University positionResearch Associate InstitutesMedical Research Council Laboratory of Molecular Biology Home pagehttps://www2.mrc-lmb.cam.ac.uk/groups/... Research ThemeInterestsAcid-Sensing Ion Channels (ASICs) play central roles in chronic pain and other acidosis-related pathologies. The focus of my research is to dissect their physiological and molecular function in neurons, muscle and the intestine and how mutations in these channels affect behaviour and metabolism. I use Two-Electrode-Voltage Clamp (TEVC) in Xenopus oocytes for characterising these ASICs in vitro. And in order to investigate and dissect physiological processes in vivo, I use the 1 mm long transparent nematode C. elegans which present an excellent model organism for a systems-level understanding of genetics, physiology and behaviour due to its complete connectome, vast behavioural repertoire and due to its transparency physiological processes can be easily observed in real time using optical indicators. Research Focus
EquipmentBehavioural analysis Calcium imaging Confocal microscopy Electrophysiological recording techniques Fluorescence microscopy Microscopy Recombinant protein expression Collaborators
Associated News ItemsKey publicationsKaulich, E., McCubbin, P.T.N., Schafer W.R., and Walker D.S. (2022), “Physiological insight into the conserved properties of Caenorhabditis elegans acid-sensing DEG/ENaCs” Physiol. J. DOI: 10.1113/JP283238 Kaulich E., Carroll T., Ackley BD., Tang YQ, Hardege I, Nehrke K., Schafer WR, Walker DS (2022), “Distinct roles for two Caenorhabditis elegans acid-sensing ion channels in an ultradian clock.” eLife DOI: 10.7554/eLife.75837 Kaulich E, Walker DS, Tang YQ, Schafer WR. (2021), “The Caenorhabditis elegans tmc-1 is involved in egg-laying inhibition in response to harsh touch. ” MicroPubl Biol. Giordano-Santini R, Kaulich E, Galbraith KM, Ritchie FK, Wang W, Li, Z, Hilliard MA. (2020), “Fusogen-mediated neuron-neuron fusion disrupts neural circuit connectivity and alters animal behavior. ” Proc Natl Acad Sci U S A 117(37), 23054-23065. Roumazeilles L*, Dokalis N*, Kaulich E*, Lelievre V (2018), “It is all about the support - The role of the extracellular matrix in regenerating axon guidance. ” Cell Adh Migr. 12(2):87-92. Publications2021Fasseas MK, Grover M, Drury F, Essmann CL, Kaulich E, Schafer WR, Barkoulas M. (2021), “Chemosensory Neurons Modulate the Response to Oomycete Recognition in Caenorhabditis elegans. ” Cell Rep. 34(2):108604. Martinez-Marmol R, Giordano-Santini R, Kaulich E, Cho A, Riyadh MA, Robinson E, Balistreri G, Meunier FA, Ke YD, Ittner LM, Hilliard MA (2021), “The SARS-CoV-2 spike (S) and the orthoreovirus p15 cause neuronal and glial fusion.” bioRxiv Pre-print |