Dr Eva Kaulich

Interests

Acid-Sensing Ion Channels (ASICs) play central roles in chronic pain and other acidosis-related pathologies. The focus of my research is to dissect their physiological and molecular function in neurons, muscle and the intestine and how mutations in these channels affect behaviour and metabolism. I use Two-Electrode-Voltage Clamp (TEVC) in Xenopus oocytes for characterising these ASICs in vitro. And in order to investigate and dissect physiological processes in vivo, I use the 1 mm long transparent nematode C. elegans which present an excellent model organism for a systems-level understanding of genetics, physiology and behaviour due to its complete connectome, vast behavioural repertoire and due to its transparency physiological processes can be easily observed in real time using optical indicators.

Research Focus

Keywords

C. elegans

DEG/ENaC

acid-sensing ion channels

protonergic transmission

metabolism

Clinical conditions

Acidosis-related pathologies

Genetic disorders

Equipment

Behavioural analysis

Calcium imaging

Confocal microscopy

Electrophysiological recording techniques

Fluorescence microscopy

Microscopy

Recombinant protein expression

Collaborators

United Kingdom

Dr Michalis Barkoulas Web: https://www.imperial.ac.uk/people/...

International

Dionysia Petratou Web: https://tavernarakislab.gr

Prof. Brian D. Ackley Web: https://ackleylab.wordpress.com/the-ack...

Dr. Cassi Estrem Web: https://flavell.mit.edu

A/Prof. Steven Flavell Web: https://flavell.mit.edu

Dr. Keith Nehrke Web: https://www.nehrkelab.com

Prof. Nektarios Tavernarakis Web: https://tavernarakislab.gr

Associated News Items


    Key publications

    Fasseas MK, Grover M, Drury F, Essmann CL, Kaulich E, Schafer WR, Barkoulas M. (2021), “Chemosensory Neurons Modulate the Response to Oomycete Recognition in Caenorhabditis elegans. ” Cell Rep. 34(2):108604.

    Kaulich E, Walker DS, Tang YQ, Schafer WR. (2021), “The Caenorhabditis elegans tmc-1 is involved in egg-laying inhibition in response to harsh touch. ” MicroPubl Biol.

    Kaulich E., Ackley BD., Tang YQ, Hardege I, Schafer WR, Walker DS (in submission), “Distinct roles for two Caenorhabditis elegans acid-sensing ion channels in an ultradian clock” biorxiv (Preprint)

    Martinez-Marmol R, Giordano-Santini R, Kaulich E, Cho A, Riyadh MA, Robinson E, Balistreri G, Meunier FA, Ke YD, Ittner LM, Hilliard MA (2021), “The SARS-CoV-2 spike (S) and the orthoreovirus p15 cause neuronal and glial fusion.” bioRxiv Pre-print

    Giordano-Santini R, Kaulich E, Galbraith KM, Ritchie FK, Wang W, Li, Z, Hilliard MA. (2020), “Fusogen-mediated neuron-neuron fusion disrupts neural circuit connectivity and alters animal behavior. ” Proc Natl Acad Sci U S A 117(37), 23054-23065.

    Roumazeilles L*, Dokalis N*, Kaulich E*, Lelievre V (2018), “It is all about the support - The role of the extracellular matrix in regenerating axon guidance. ” Cell Adh Migr. 12(2):87-92.