Dr Evan Reid University positionWellcome Trust Senior Research Fellow Dr Evan Reid is pleased to consider applications from prospective PhD students. DepartmentsDepartment of Medical Genetics InstitutesCambridge Institute for Medical Research (CIMR) Home pagehttp://www.cimr.cam.ac.uk/researc... Research ThemeInterestsMy group's research is focussed on the hereditary spastic paraplegias, genetic conditions where the corticospinal tract axons degenerate. HSPs selectively involve axons while sparing the neuronal cell bodies, so we study them to understand molecular mechanisms crucial for axonal maintenance and degeneration. Our research has evolved from gene mapping and identification studies to concentrate on cell biological studies, particularly in the area of the role of HSP proteins in membrane traffic. The overall aim of this research is to understand the normal function of selected HSP proteins, to determine how abnormality of these functions leads to the disease, and to use this knowledge to rationally design new therapeutic strategies for the disorder. Research Focus
EquipmentCell culture Cellular functional assays Confocal microscopy Fluorescence microscopy Protein purification Recombinant protein expression Collaborators
Associated News Items
Key publicationsAllison R, Lumb JH, Fassier C, Connell JW, Ten Martin D, Seaman MN, Hazan J, Reid E (2013), “An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.” J Cell Biol 202(3):527-43 Details Montenegro G, Rebelo AP, Connell J, Allison R, Babalini C, D'Aloia M, Montieri P, Schüle R, Ishiura H, Price J, Strickland A, Gonzalez MA, Baumbach-Reardon L, Deconinck T, Huang J, Bernardi G, Vance JM, Rogers MT, Tsuji S, De Jonghe P, Pericak-Vance MA, Schöls L, Orlacchio A, Reid E, Züchner S (2012), “Mutations in the ER-shaping protein reticulon 2 cause the axon-degenerative disorder hereditary spastic paraplegia type 12.” J Clin Invest 122(2):538-44 Details Blackstone C, O'Kane CJ, Reid E (2011), “Hereditary spastic paraplegias: membrane traffic and the motor pathway.” Nat Rev Neurosci 12(1):31-42 Details Wang X, Shaw RW, Tsang HT, Reid E, O’Kane CJ (2007), “Spichthyin, the Drosophila homolog of ichthyin and SPG6, is an inhibitor of BMP signaling, and can impair axonal transport” Nature Neuroscience 10:177-85 Sanderson CM, Connell JW, Edwards TL, Bright NA, Duley S, Thompson A, Luzio JP, Reid E (2006), “Spastin and atlastin, two proteins mutated in autosomal-dominant hereditary spastic paraplegia, are binding partners” Human Molecular Genetics 15:307-18 Details Reid E, Kloos M, Ashley-Koch A, Hughes L, Bevan S, Svenson IK, Lennon Graham F, Gaskell PC, Dearlove A, Pericak-Vance M, Rubinsztein DC, Marchuk DA (2002), “A kinesin heavy chain (KIF5A) mutation in hereditary spastic paraplegia (SPG10)” Am J Hum Genet 71:1189-94 Publications2013Freeman C, Seaman MN, Reid E (2013), “The hereditary spastic paraplegia protein strumpellin: characterisation in neurons and of the effect of disease mutations on WASH complex assembly and function.” Biochim Biophys Acta 1832(1):160-73 Details Timmerman V, Clowes VE, Reid E (2013), “Overlapping molecular pathological themes link Charcot-Marie-Tooth neuropathies and hereditary spastic paraplegias.” Exp Neurol 246:14-25 Details 2012Karle KN, Möckel D, Reid E, Schöls L (2012), “Axonal transport deficit in a KIF5A( -/- ) mouse model.” Neurogenetics 13(2):169-79 Details Lumb JH, Connell JW, Allison R, Reid E (2012), “The AAA ATPase spastin links microtubule severing to membrane modelling.” Biochim Biophys Acta 1823(1):192-7 Details O'Sullivan NC, Jahn TR, Reid E, O'Kane CJ (2012), “Reticulon-like-1, the Drosophila orthologue of the hereditary spastic paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons.” Hum Mol Genet 21(15):3356-65 Details 2011Hopkins TG, Maher ER, Reid E, Marciniak SJ (2011), “Recurrent pneumothorax.” Lancet 377(9777):1624 Details Schiel JA, Park K, Morphew MK, Reid E, Hoenger A, Prekeris R (2011), “Endocytic membrane fusion and buckling-induced microtubule severing mediate cell abscission.” J Cell Sci 124(Pt 9):1411-24 Details 2010Harbour ME, Breusegem SY, Antrobus R, Freeman C, Reid E, Seaman MN (2010), “The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics.” J Cell Sci Details 2009Connell JW, Lindon C, Luzio JP, Reid E (2009), “Spastin couples microtubule severing to membrane traffic in completion of cytokinesis and secretion.” Traffic 10(1):42-56 Details Edwards TL, Clowes VE, Tsang HT, Connell JW, Sanderson CM, Luzio JP, Reid E (2009), “Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5.” Biochem J Details Smith BN, Bevan S, Vance C, Renwick P, Wilkinson P, Proukakis C, Squitieri F, Berardelli A, Warner TT, Reid E, Shaw CE (2009), “Four novel SPG3A/atlastin mutations identified in autosomal dominant hereditary spastic paraplegia kindreds with intra-familial variability in age of onset and complex phenotype.” Clin Genet 75(5):485-9 Details Tsang HT, Edwards TL, Wang X, Connell JW, Davies RJ, Durrington HJ, O'Kane CJ, Luzio JP, Reid E (2009), “The hereditary spastic paraplegia proteins NIPA1, spastin and spartin are inhibitors of mammalian BMP signaling.” Hum Mol Genet Details 2007Beetz C, Nygren AO, Deufel T, Reid E (2007), “An SPG3A whole gene deletion neither co-segregates with disease nor modifies phenotype in a hereditary spastic paraplegia family with a pathogenic SPG4 deletion.” Neurogenetics 8(4):317-8 Details Douglas J, Cilliers D, Coleman K, Tatton-Brown K, Barker K, Bernhard B, Burn J, Huson S, Josifova D, Lacombe D, Malik M, Mansour S, Reid E, Cormier-Daire V, Cole T, Childhood Overgrowth Collaboration, Rahman N (2007), “Mutations in RNF135, a gene within the NF1 microdeletion region, cause phenotypic abnormalities including overgrowth.” Nat Genet 39(8):963-5 Details Marini C, Mei D, Temudo T, Ferrari AR, Buti D, Dravet C, Dias AI, Moreira A, Calado E, Seri S, Neville B, Narbona J, Reid E, Michelucci R, Sicca F, Cross HJ, Guerrini R (2007), “Idiopathic epilepsies with seizures precipitated by fever and SCN1A abnormalities.” Epilepsia 48(9):1678-85 Details Moumoulidis I, Axon P, Baguley D, Reid E (2007), “A review on the genetics of otosclerosis.” Clin Otolaryngol 32(4):239-47 Details Valdmanis PN, Meijer IA, Reynolds A, Lei A, MacLeod P, Schlesinger D, Zatz M, Reid E, Dion PA, Drapeau P, Rouleau GA (2007), “Mutations in the KIAA0196 gene at the SPG8 locus cause hereditary spastic paraplegia.” Am J Hum Genet 80(1):152-61 Details Wang X, Shaw WR, Tsang HT, Reid E, O'Kane CJ (2007), “Drosophila spichthyin inhibits BMP signaling and regulates synaptic growth and axonal microtubules.” Nat Neurosci 10(2):177-85 Details 2006Kersey JP, Vivian AJ, Reid E (2006), “A report of paracentric inversion of chromosome 8 in Moebius syndrome.” Ophthalmic Genet 27(1):29-31 Details Tsang HT, Connell JW, Brown SE, Thompson A, Reid E, Sanderson CM (2006), “A systematic analysis of human CHMP protein interactions: additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex.” Genomics 88(3):333-46 Details 2005Reid E, Connell J, Edwards TL, Duley S, Brown SE, Sanderson CM (2005), “The hereditary spastic paraplegia protein spastin interacts with the ESCRT-III complex-associated endosomal protein CHMP1B.” Hum Mol Genet 14(1):19-38 Details 2004Shaw-Smith CJ, Lewis SJ, Reid E (2004), “X-linked adrenoleukodystrophy presenting as autosomal dominant pure hereditary spastic paraparesis.” J Neurol Neurosurg Psychiatry 75(5):686-8 Details 2003Blumen SC, Bevan S, Abu-Mouch S, Negus D, Kahana M, Inzelberg R, Mazarib A, Mahamid A, Carasso RL, Slor H, Withers D, Nisipeanu P, Navon R, Reid E (2003), “A locus for complicated hereditary spastic paraplegia maps to chromosome 1q24-q32.” Ann Neurol 54(6):796-803 Details King AL, Moodie SJ, Fraser JS, Curtis D, Reid E, Dearlove AM, Ciclitira PJ (2003), “Coeliac disease: investigation of proposed causal variants in the CTLA4 gene region.” Eur J Immunogenet 30(6):427-32 Details Proukakis C, Auer-Grumbach M, Wagner K, Wilkinson PA, Reid E, Patton MA, Warner TT, Crosby AH (2003), “Screening of patients with hereditary spastic paraplegia reveals seven novel mutations in the SPG4 (Spastin) gene.” Hum Mutat 21(2):170 Details Reid E (2003), “Many pathways lead to hereditary spastic paraplegia.” Lancet Neurol 2(4):210 Details Reid E (2003), “Science in motion: common molecular pathological themes emerge in the hereditary spastic paraplegias.” J Med Genet 40(2):81-6 Details Yip AG, Dürr A, Marchuk DA, Ashley-Koch A, Hentati A, Rubinsztein DC, Reid E (2003), “Meta-analysis of age at onset in spastin-associated hereditary spastic paraplegia provides no evidence for a correlation with mutational class.” J Med Genet 40(9):e106 Details 2002King AL, Moodie SJ, Fraser JS, Curtis D, Reid E, Dearlove AM, Ellis HJ, Ciclitira PJ (2002), “CTLA-4/CD28 gene region is associated with genetic susceptibility to coeliac disease in UK families.” J Med Genet 39(1):51-4 Details 2001Reid E, Escayg A, Dearlove AM, Meisler MH, Rubinsztein DC (2001), “The spastic paraplegia SPG10 locus: narrowing of critical region and exclusion of sodium channel gene SCN8A as a candidate.” J Med Genet 38(1):65-7 Details 2000Baralle D, Dearlove AM, Beach R, Ffrench-Constant C, Reid E (2000), “Benign familial infantile convulsions: report of a UK family and confirmation of genetic heterogeneity.” J Med Genet 37(10):E31 Details de Lange RP, Bolt J, Reid E, da Silva R, Shaw DJ, Clair DM (2000), “Screening British CADASIL families for mutations in the NOTCH3 gene.” J Med Genet 37(3):224-5 Details Lindsey JC, Lusher ME, McDermott CJ, White KD, Reid E, Rubinsztein DC, Bashir R, Hazan J, Shaw PJ, Bushby KM (2000), “Mutation analysis of the spastin gene (SPG4) in patients with hereditary spastic paraparesis.” J Med Genet 37(10):759-65 Details Reid E, Dearlove AM, Osborn O, Rogers MT, Rubinsztein DC (2000), “A locus for autosomal dominant "pure" hereditary spastic paraplegia maps to chromosome 19q13.” Am J Hum Genet 66(2):728-32 Details 1999Lowe GD, Rumley A, Woodward M, Reid E, Rumley J (1999), “Activated protein C resistance and the FV:R506Q mutation in a random population sample--associations with cardiovascular risk factors and coagulation variables.” Thromb Haemost 81(6):918-24 Details Reid E (1999), “The hereditary spastic paraplegias.” J Neurol 246(11):995-1003 Details Reid E, Dearlove AM, Rhodes M, Rubinsztein DC (1999), “A new locus for autosomal dominant "pure" hereditary spastic paraplegia mapping to chromosome 12q13, and evidence for further genetic heterogeneity.” Am J Hum Genet 65(3):757-63 Details Reid E, Dearlove AM, Whiteford ML, Rhodes M, Rubinsztein DC (1999), “Autosomal dominant spastic paraplegia: refined SPG8 locus and additional genetic heterogeneity.” Neurology 53(8):1844-9 Details Reid E, Grayson C, Rogers MT, Rubinsztein DC (1999), “Locus-phenotype correlations in autosomal dominant pure hereditary spastic paraplegia. A clinical and molecular genetic study of 28 United Kingdom families.” Brain 122 ( Pt 9):1741-55 Details Reid E, Grayson C, Rubinsztein DC, Rogers MT, Rubinsztein JS (1999), “Subclinical cognitive impairment in autosomal dominant "pure" hereditary spastic paraplegia.” J Med Genet 36(10):797-8 Details 1998Reid E, Douglas F, Crow Y, Hollman A, Gibson J (1998), “Autosomal dominant juvenile recurrent parotitis.” J Med Genet 35(5):417-9 Details 1997Lowe GD, Rumley A, Woodward M, Reid E (1997), “Oral contraceptives and venous thromboembolism.” Lancet 349(9065):1623 Details Reid E (1997), “Pure hereditary spastic paraplegia.” J Med Genet 34(6):499-503 Details 1996Ahmed MA, Reid E, Cooke A, Arngrímsson R, Tolmie JL, Stephenson JB (1996), “Familial hemiplegic migraine in the west of Scotland: a clinical and genetic study of seven families.” J Neurol Neurosurg Psychiatry 61(6):616-20 Details Reid E, Morrison N, Barron L, Boyd E, Cooke A, Fielding D, Tolmie JL (1996), “Familial Wolf-Hirschhorn syndrome resulting from a cryptic translocation: a clinical and molecular study.” J Med Genet 33(3):197-202 Details |