Dr Claire Durrant (née Harwell)
https://www.linkedin.com/in/clai... (personal home page)
My research aims to model aspects of Alzheimer's disease pathology using organotypic hippocampal slice cultures from APP mutant mice. By maintaining slice cultures from TgCRND8 mice for several months in culture, the progressive accumulation of Aβ and loss of presynaptic proteins can be observed (Harwell & Coleman 2016). As synapse loss is the best correlate of cognitive deficits in Alzheimer's disease, understanding the mechanisms behind this loss may serve to identify novel therapeutic targets. Current work is seeking to identify the causes of presynaptic protein loss in our model as well as attempting to rescue this phenotype using a number of pharmacological and genetic interventions.
Further work seeks to examine other neurodegenerative disease models using the organotypic slice culture technique, with the aim of identifying common targets and mechanisms.
Organotypic Slice Culture
No collaborators listed
Associated News Items
Sheppard O, Coleman MP, Durrant CS (2019), “Lipopolysaccharide-induced neuroinflammation induces presynaptic disruption through a direct action on brain tissue involving microglia-derived interleukin 1 beta” Journal of Neuroinflammation 16:106
Harwell CS, Coleman MP (2016), “Synaptophysin depletion and intraneuronal Aβ in organotypic hippocampal slice cultures from huAPP transgenic mice” Mol Neurodegener 11:44
Adalbert R, Milde S, Durrant C, Ando K, Stygelbout V, Yilmaz Z, Gould S, Brion JP, Coleman MP (2018), “Interaction between a MAPT variant causing frontotemporal dementia and mutant APP affects axonal transport” Neurobiology of Aging 68: 68-75