Dr Bart Nieuwenhuis University positionResearch Associate DepartmentsDepartment of Clinical Neurosciences InstitutesCambridge Centre for Brain Repair Home pageResearch ThemesInterestsInjury to the brain and spinal cord has devastating consequences because adult neurons in the central nervous system (CNS) do not regenerate. Gene therapy is a promising tool to promote axon regeneration. My research makes contributions to the field of gene therapy and restorative neuroscience: 1 – Gene therapy: Optimising the delivery of transgenes in the brain, spinal cord, and eyes, using adeno-associated viral vectors 2 – Laser axotomy: Identification and validation of potential axon regeneration targets in CNS neurons 3 – Axon transport: Increasing the growth machinery in the axon of CNS neurons to promote regeneration Research Focus
EquipmentBehavioral analysis Cell culture Fluorescence microscopy Immunohistochemistry Live cell imaging Viral expression vectors Collaborators
Associated News ItemsPublications2023Nieuwenhuis B, Laperrousaz E, Tribble JR, Verhaagen J, Fawcett JW, Martin KR, Williams PA, Osborne O (2023), “Improving adeno-associated viral (AAV) vector-mediated transgene expression in retinal ganglion cells: comparison of five promoters” Gene Therapy 2022Nieuwenhuis B, Eva R (2022), “Promoting axon regeneration in the central nervous system by increasing PI3-kinase signaling” Neural Regen Res 17(6): 1172-1182 2021Cross T, Navarange R, Son J, Burr W, Singh A, Zhang K, Rusu M, Gkoutzis K, Osborne A, Nieuwenhuis B (2021), “Simple RGC: ImageJ plugins for counting retinal ganglion cells and determining the transduction efficiency of viral vectors in retinal wholemounts” Journal of Open Research Software 9(1), p.15. Loreto A, Angeletti C, Gu W, Osborne A, Nieuwenhuis B, Gilley J, Arthur-Farraj P, Merlini E, Amici A, Luo Z, Hartley-Tassell L, Ve T, Desrochers LM, Wang Q, Kobe B, Orsomando G, Coleman MP (2021), “Neurotoxin-mediated potent activation of the axon degeneration regulator SARM1” eLife Nieuwenhuis B, Haenzi B, Hilton S, Carnicer-Lombarte A, Hobo B, Verhaagen J, Fawcett JW (2021), “Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters” Gene Therapy 28, 56–74 Petrova V, Nieuwenhuis B, Fawcett JW, Eva R (2021), “Axonal organelles as molecular platforms for axon growth and regeneration after injury” Int. J. Mol. Sci. Yang S, Gigout S, Molinaro A, Naito-Matsui Y, Hilton S, Foscarin S, Nieuwenhuis B, Tan CL, Verhaagen J, Pizzorusso T, Saksida LM, Bussey TM, Kitagawa H, Kwok JCF, Fawcett JW (2021), “Chondroitin 6-sulphate is required for neuroplasticity and memory in ageing” Mol Psychiatry 2020Nieuwenhuis B, Barber AC, Evans RS, Pearson CS, Fuchs J, MacQueen AR, van Erp S, Haenzi B, Hulshof LA, Osborne A, Conceicao R, Khatib TZ, Deshpande SS, Cave J, ffrench-Constant C, Smith PD, Okkenhaug K, Eickholt BJ, Martin KR, Fawcett JW, Eva R (2020), “PI 3?kinase delta enhances axonal PIP3 to support axon regeneration in the adult CNS” EMBO Mol Med e11674 Nieuwenhuis B, Eva R (2020), “ARF6 and Rab11 as intrinsic regulators of axon regeneration” Small GTPases 11(6): 392-401 PDF 2018Nieuwenhuis B, Eva R (2018), “Linking axon transport to regeneration using in vitro laser axotomy” Neural Regen Res 13(3): 410-412 PDF Nieuwenhuis B, Haenzi B, Andrews MR, Verhaagen J, Fawcett JW (2018), “Integrins promote axonal regeneration after injury of the nervous system” Biological Reviews PDF 2014Mecollari V, Nieuwenhuis B, Verhaagen J (2014), “A perspective on the role of class III semaphorin signaling in central nervous system trauma.” Front Cell Neurosci 8:328 PDF Details |