Dr Raymond Bujdoso University positionSenior Lecturer Dr Raymond Bujdoso is pleased to consider applications from prospective PhD students. DepartmentsDepartment of Veterinary Medicine Home pagehttp://www.vet.cam.ac.uk/researc... Research ThemesInterestsPrion diseases such as scrapie of sheep and goats, BSE of cattle and CJD of humans are transmissible neurodegenerative diseases. The research of our prion group is concerned with providing knowledge to try and answer some of the important questions related to aspects of prion disease pathogenesis, diagnosis and therapeutic strategy. These questions include defining the nature of the infectious agent, determining the molecular basis of prion strain diversity, understanding the mechanism of protein misfolding as PrPC converts into PrPSc, and identification of molecular targets for diagnostic and potential therapeutic interventions. These aspects of PrP biology are being pursued in our attempts to identify and quantify abnormal PrP in the blood of individuals with pre-clinical prion disease. These studies underpin our efforts to develop an effective ante-mortem blood test for prion disease. Research Focus
EquipmentCell culture Computational modelling Flow cytometry Immunoassays Immunohistochemistry Protein purification Recombinant protein expression Collaborators
Associated News ItemsKey publicationsThackray AM, Hopkins L, Bujdoso R (2007), “Proteinase K-sensitive disease-associated ovine prion protein revealed by conformation-dependent immunoassay” Biochem J 401:475-483 Details Thackray AM, Fitzmaurice TJ, Hopkins L, Bujdoso R (2006), “Ovine plasma prion protein levels show genotypic variation detected by C-terminal epitopes not exposed in cell-surface PrPC.” Biochem J 400(2):349-58 Details Bujdoso R, Burke DF, Thackray AM (2005), “Structural differences between allelic variants of the ovine prion protein revealed by molecular dynamics simulations” Proteins 61:840-849 Details Publications2014Thackray AM, Zhang C, Arndt T, Bujdoso R (2014), “Cytosolic PrP can participate in prion-mediated toxicity.” J Virol Details 2013Beck KE, Thorne L, Lockey R, Vickery CM, Terry LA, Bujdoso R, Spiropoulos J (2013), “Strain typing of classical scrapie by transgenic mouse bioassay using protein misfolding cyclic amplification to replace primary passage.” PLoS One 8(3):e57851 Details Li W, Bell NA, Hernández-Ainsa S, Thacker VV, Thackray AM, Bujdoso R, Keyser UF (2013), “Single protein molecule detection by glass nanopores.” ACS Nano 7(5):4129-34 Details 2012Thackray AM, Hopkins L, Lockey R, Spiropoulos J, Bujdoso R (2012), “Propagation of ovine prions from "poor" transmitter scrapie isolates in ovine PrP transgenic mice.” Exp Mol Pathol 92(1):167-74 Details Thackray AM, Lockey R, Beck KE, Spiropoulos J, Bujdoso R (2012), “Evidence for co-infection of ovine prion strains in classical scrapie isolates.” J Comp Pathol 147(2-3):316-29 Details Thackray AM, Muhammad F, Zhang C, Denyer M, Spiropoulos J, Crowther DC, Bujdoso R (2012), “Prion-induced toxicity in PrP transgenic Drosophila.” Exp Mol Pathol 92(2):194-201 Details Thackray AM, Muhammad F, Zhang C, Di Y, Jahn TR, Landgraf M, Crowther DC, Evers JF, Bujdoso R (2012), “Ovine PrP transgenic Drosophila show reduced locomotor activity and decreased survival.” Biochem J 444(3):487-95 Details 2011Thackray AM, Hopkins L, Lockey R, Spiropoulos J, Bujdoso R (2011), “Emergence of multiple prion strains from single isolates of ovine scrapie.” J Gen Virol 92(Pt 6):1482-91 Details 2009Zabel M, Greenwood C, Thackray AM, Pulford B, Rens W, Bujdoso R (2009), “Perturbation of T-cell development by insertional mutation of a PrP transgene.” Immunology 127(2):226-36 Details 2008Fitzmaurice TJ, Burke DF, Hopkins L, Yang S, Yu S, Sy MS, Thackray AM, Bujdoso R (2008), “The stability and aggregation of ovine prion protein associated with classical and atypical scrapie correlates with the ease of unwinding of helix-2.” Biochem J 409(2):367-75 Details Thackray AM, Hopkins L, Spiropoulos J, Bujdoso R (2008), “Molecular and transmission characteristics of primary-passaged ovine scrapie isolates in conventional and ovine PrP transgenic mice.” J Virol 82(22):11197-207 Details von Poser-Klein C, Flechsig E, Hoffmann T, Schwarz P, Harms H, Bujdoso R, Aguzzi A, Klein MA (2008), “Alteration of B-cell subsets enhances neuroinvasion in mouse scrapie infection.” J Virol 82(7):3791-5 Details Yang S, Thackray AM, Fitzmaurice TJ, Bujdoso R (2008), “Copper-induced structural changes in the ovine prion protein are influenced by a polymorphism at codon 112.” Biochim Biophys Acta 1784(4):683-92 Details 2007Thackray AM, Hopkins L, Klein MA, Bujdoso R (2007), “Mouse-adapted ovine scrapie prion strains are characterized by different conformers of PrPSc.” J Virol 81(22):12119-27 Details 2006Encke J, Bernardin J, Geib J, Barbakadze G, Bujdoso R, Stremmel W (2006), “Genetic vaccination with Flt3-L and GM-CSF as adjuvants: Enhancement of cellular and humoral immune responses that results in protective immunity in a murine model of hepatitis C virus infection.” World J Gastroenterol 12(44):7118-25 Details Thackray AM, Bujdoso R (2006), “Elevated PrPC expression predisposes to increased HSV-1 pathogenicity.” Antivir Chem Chemother 17(1):41-52 Details 2005Thackray AM, Ryder SJ, Bujdoso R (2005), “Modification of blood cell PrP epitope exposure during prion disease.” Biochem J 390(Pt 2):563-71 Details 2004Thackray AM, McKenzie AN, Klein MA, Lauder A, Bujdoso R (2004), “Accelerated prion disease in the absence of interleukin-10.” J Virol 78(24):13697-707 Details Thackray AM, Yang S, Wong E, Fitzmaurice TJ, Morgan-Warren RJ, Bujdoso R (2004), “Conformational variation between allelic variants of cell-surface ovine prion protein.” Biochem J 381(Pt 1):221-9 Details Wong E, Thackray AM, Bujdoso R (2004), “Copper induces increased beta-sheet content in the scrapie-susceptible ovine prion protein PrPVRQ compared with the resistant allelic variant PrPARR.” Biochem J 380(Pt 1):273-82 Details 2003Celer V, Blazek D, Navrátilová I, Skládal P, Blacklaws B, Bujdoso R (2003), “Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus.” Folia Microbiol (Praha) 48(3):435-40 Details Thackray AM, Klein MA, Bujdoso R (2003), “Subclinical prion disease induced by oral inoculation.” J Virol 77(14):7991-8 Details Thackray AM, Madec JY, Wong E, Morgan-Warren R, Brown DR, Baron T, Bujdoso R (2003), “Detection of bovine spongiform encephalopathy, ovine scrapie prion-related protein (PrPSc) and normal PrPc by monoclonal antibodies raised to copper-refolded prion protein.” Biochem J 370(Pt 1):81-90 Details 2002Thackray AM, Bujdoso R (2002), “PrP(c) expression influences the establishment of herpes simplex virus type 1 latency.” J Virol 76(5):2498-509 Details Thackray AM, Klein MA, Aguzzi A, Bujdoso R (2002), “Chronic subclinical prion disease induced by low-dose inoculum.” J Virol 76(5):2510-7 Details Thackray AM, Knight R, Haswell SJ, Bujdoso R, Brown DR (2002), “Metal imbalance and compromised antioxidant function are early changes in prion disease.” Biochem J 362(Pt 1):253-8 Details 2001Smith JR, Thackray AM, Bujdoso R (2001), “Reduced herpes simplex virus type 1 latency in Flt-3 ligand-treated mice is associated with enhanced numbers of natural killer and dendritic cells.” Immunology 102(3):352-8 Details 1998O'Leary MT, Bujdoso R, Blakemore WF (1998), “Rejection of wild-type and genetically engineered major histocompatibility complex-deficient glial cell xenografts in the central nervous system results in bystander demyelination and Wallerian degeneration.” Neuroscience 85(1):269-80 Details 1995Bujdoso R, Williamson M, Roy D, Hunt P, Blacklaws B, Sargan D, McConnell I (1995), “Molecular cloning and expression of DNA encoding ovine interleukin 2.” Cytokine 7(3):223-31 Details 1994Balogh S, Gönczy T, Bujdosó R, Kullmann L (1994), “New walking aid for primary mobilization of an infant with deficiency of all four limbs.” Prosthet Orthot Int 18(1):49-51 Details Colston A, McConnell I, Bujdoso R (1994), “Cloning and expression in Escherichia coli of DNA encoding a 60 kDa stress protein of Mycobacterium paratuberculosis, the causative agent of Johne's disease.” Microbiology 140 ( Pt 12):3329-36 Details Evans CW, Lund BT, McConnell I, Bujdoso R (1994), “Antigen recognition and activation of ovine gamma delta T cells.” Immunology 82(2):229-37 Details Hunt P, Colston A, Bujdoso R (1994), “Nomenclature of mycobacterial stress proteins (65 kDa antigens) and other members of the Hsp60 family.” Trends Microbiol 2(8):298 Details 1993Bujdoso R, Lund BT, Evans CW, McConnell I (1993), “Different rates of interleukin 2 receptor expression by ovine gamma/delta and alpha/beta T cells.” Vet Immunol Immunopathol 39(1-3):109-14 Details Lund BT, Evans CW, Davis WC, McConnell I, Bujdoso R (1993), “Expression of T19 (WC1) molecules by ovine lymphocytes.” Vet Immunol Immunopathol 39(1-3):145-53 Details 1992Bujdoso R, Sargan D, Williamson M, McConnell I (1992), “Cloning of a cDNA encoding the ovine interleukin-2 receptor 55-kDa protein, CD25.” Gene 113(2):283-4 Details 1991Bujdoso R, Sargan D, Ballingall K, Sanderson A (1991), “The use of flow cytometry to detect transfected gene products.” Methods Mol Biol 7:361-77 Details 1990Bujdoso R, Harkiss G, Hopkins J, McConnell I (1990), “Afferent lymph dendritic cells: a model for antigen capture and presentation in vivo.” Int Rev Immunol 6(2-3):177-86 Details Bujdoso R, Young P, Hopkins J, McConnell I (1990), “IL-2-like activity in lymph fluid following in vivo antigen challenge.” Immunology 69(1):45-51 Details Dutia BM, Hopkins J, Allington MP, Bujdoso R, McConnell I (1990), “Characterization of monoclonal antibodies specific for alpha- and beta-chains of sheep MHC class II.” Immunology 70(1):27-32 Details 1989Bujdoso R, Hopkins J, Dutia BM, Young P, McConnell I (1989), “Characterization of sheep afferent lymph dendritic cells and their role in antigen carriage.” J Exp Med 170(4):1285-301 Details Bujdoso R, Young P, Harkiss GD, McConnell I (1989), “Antigen presentation in the sheep: generation of antigen-specific T-cell lines.” Immunology 66(4):559-64 Details Bujdoso R, Young P, Hopkins J, Allen D, McConnell I (1989), “Non-random migration of CD4 and CD8 T cells: changes in the CD4: CD8 ratio and interleukin 2 responsiveness of efferent lymph cells following in vivo antigen challenge.” Eur J Immunol 19(10):1779-84 Details Hopkins J, Dutia BM, Bujdoso R, McConnell I (1989), “In vivo modulation of CD1 and MHC class II expression by sheep afferent lymph dendritic cells. Comparison of primary and secondary immune responses.” J Exp Med 170(4):1303-18 Details 1986Bujdoso R, Munro AJ (1986), “The response of T cells to soluble protein antigens and fixed antigen-presenting cells.” Immunology 59(1):51-5 Details |