James Higham


University position

PhD student
Supervised by Dr David Bulmer

Departments

Department of Pharmacology

Email

jph87@cam.ac.uk

Home page

https://www.neuroscience.cam.ac.uk/directo...

Research Theme

Cellular and Molecular Neuroscience

Interests

My PhD with Dr David Bulmer is primarily focussed on investigating interactions between novel inflammatory mediators and nociceptive neurons in culture. I use genetically engineered neurons (in a collaboration with Prof John Wood and Dr James Cox, UCL) and pharmacological tools in tandem with calcium imaging, patch-clamp electrophysiology and immunohistochemistry.

At the University of Bristol, I used Drosophila to model the neuronal basis of behavioural defects in Alzheimer's disease with Dr James Hodge. To do this, we used genetic engineering, behavioural assays, calcium imaging and patch-clamp electrophysiology.

I was also under the supervision of Prof Neil Marrion, who taught me patch-clamp electrophysiological recording. I used outside-out and inside-out patch recording to determine the pharmacological and biophysical properties of a novel heteromeric calcium-activated potassium channel.

Research Focus

Keywords

Nociceptor

Inflammation

Ion channels

Neurophysiology

Clinical conditions

Inflammatory bowel disease

No direct clinical relevance

Equipment

Calcium imaging

Cell culture

Electrophysiological recording techniques

Immunohistochemistry

Whole cell patch clamp

Collaborators

United Kingdom

Neil Marrion Web: http://www.bristol.ac.uk/phys-ph...

Associated News Items


    Publications

    2019

    Edgar Buhl*, James P Higham*, James J L Hodge (2019), “Alzheimer's Disease-Associated Tau Alters Drosophila Circadian Activity, Sleep and Clock Neuron Electrophysiology” Neurobiol of Dis Vol 130

    James Higham, Giriraj Sahu, Rima-Marie Wazen, Pina Colarusso, Alice Gregorie, Bartholomew S. J. Harvey, Lucy Goudswaard, Gemma Varley, David N Sheppard, Ray W Turner and Neil V Marrion (2019), “Preferred formation of heteromeric channels between co-expressed SK1 and IKCa channel subunits provides a unique pharmacological profile of Ca2+-activated K+ channels” Mol Pharm 97(5): 115-126

    James P. Higham*, Bilal R. Malik*, Edgar Buhl, Jennifer M. Dawson, Anna S. Ogier, Katie Lunnon and James J. L. Hodge (2019), “Alzheimer’s Disease Associated Genes Ankyrin and Tau Cause Shortened Lifespan and Memory Loss in Drosophila” Front Cell Neuro Vol 13; pp260

    James P. Higham†, Sergio Hidalgo†, Edgar Buhl and James J. L. Hodge* (2019), “Restoration of Olfactory Memory in Drosophila Overexpressing Human Alzheimer’s Disease Associated Tau by Manipulation of L-Type Ca2+ Channels” Front Cell Neuro Vol 13; pp409

    2017

    James P Higham (2017), “Letter: Tangled tau: Active pathology or footprint of disease?” Alzheimer's and Demantia 3(4): 658-659