Dr Richard Eva

Interests

Boosting axon regeneration after injury by targeting axon transport of growth receptors

I would like to understand the mechanisms regulating the traffic and transport of growth and survival molecules into axons. During development these molecules are present, but either decline or are absent as neurons mature and age. Consequently, injured or diseased axons in the central nervous system do not regenerate after injury. Additionally, numerous neurological diseases are associated with deficits in axonal transport, and may be caused by defects in intracellular trafficking. I have identified and characterised axonal trafficking pathways that can be manipulated to enhance axon growth and regeneration via the increased transport of growth associated molecules into axons. I am continuing to characterise newly identified signalling and trafficking pathways, and manipulating these pathways in order to increase axon regeneration after injury.

Growth Cone of an adult DRG axon expressing Rab Coupling protein GFP and labelled for beta1 integrin
Growth Cone of an adult DRG axon expressing Rab Coupling protein GFP and labelled for beta1 integrin
Click image to view full-size

Research Focus

Keywords

Neuronal Cell Biology

Axon regeneration

Organelle Traffic

Axon Transport

Neuronal Survival

Clinical conditions

spinal cord injury, axon regeneration, neurodegenerative disease

Equipment

Cell culture

Confocal microscopy

Fluorescence microscopy

Neuronal cell culture, Compartmentalised neuronal culture, In vitro axonal injury (laser axotomy), Live cell imaging, Spinning disc microscopy, Neurite outgrowth quantification, Generation of stable cell lines, siRNA treatment, Neuronal transfection (by multiple methods), Subcellular fractionation, Capture elisa, Biochemistry, Axon transport analysis, Growth cone morphology analysis, Membrane protein endocytosis and recycling assays, Gene cloning, Site directed mutagenesis,

Recombinant protein expression

Collaborators

Cambridge

Michael Coleman

Phill Hawkins

Maria Grazia Spillantini

Len Stephens

United Kingdom

Venkateswarlu Kanamarlapudi Web: http://www.swan.ac.uk/

Jim Norman Web: http://www.beatson.gla.ac.uk/Regulat...

International

Alfredo Caceres Web: http://www.immf.uncor.edu/en/inde...

Britta Eickholt Web: http://www.neurosignallab.com

Associated News Items


Key publications

Eva, R., Koseki, H., Kanamarlapudi, V., Fawcett, J.W. (in press), “EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment. ” Journal of Cell Science

Koseki, H., Donegá, M., Lam, B.Y.H., Petrova, V., Yeo, G.S.H., Kwok, J.C.F., van Erp, S., ffrench-Constant, C., Eva, R. *, Fawett, J.W. *. *Joint corresponding author. (2017), “Selective Rab11 transport and the intrinsic regenerative ability of CNS axons. ” eLife 6, e26956.

Franssen EH, Zhao RR, Koseki H, Kanamarlapudi V, Hoogenraad CC, Eva R, Fawcett JW (2015), “Exclusion of Integrins from CNS Axons Is Regulated by Arf6 Activation and the AIS.” J Neurosci 35(21):8359-75 Details

Eva R, Crisp S, Marland JR, Norman JC, Kanamarlapudi V, Ffrench-Constant C, Fawcett JW (2012), “ARF6 Directs Axon Transport and Traffic of Integrins and Regulates Axon Growth in Adult DRG Neurons.” J Neurosci 32(30):10352-10364 Details

Eva R, Dassie E, Caswell PT, Dick G, ffrench-Constant C, Norman JC, Fawcett JW (2010), “Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells.” J Neurosci 30(35):11654-69 Details

Publications

2016

Heintz TG, Eva R, Fawcett JW. (2016), “Regional Regulation of Purkinje Cell Dendritic Spines by Integrins and Eph/Ephrins. ” PLoS One 11(8):e0158558

2015

Tan CL, Kwok JC, Heller JP, Zhao R, Eva R, Fawcett JW (2015), “Full length talin stimulates integrin activation and axon regeneration.” Mol Cell Neurosci 68:1-8 Details

2014

Eva R, Fawcett J (2014), “Integrin signalling and traffic during axon growth and regeneration.” Curr Opin Neurobiol 27C:179-185 Details

Heintz TG, Heller J, Zhao R, Caceres A, Eva R, Fawcett JW (2014), “Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons.” Mol Cell Neurosci Details

2012

Eva R, Andrews MR, Franssen EH, Fawcett JW (2012), “Intrinsic mechanisms regulating axon regeneration: an integrin perspective.” Int Rev Neurobiol 106:75-104 Details

Eva R, Bouyoucef-Cherchalli D, Patel K, Cullen PJ, Banting G (2012), “IP3 3-kinase opposes NGF driven neurite outgrowth.” PLoS One 7(2):e32386 Details