Professor Roger Keynes
Professor Roger Keynes is pleased to consider applications from prospective PhD students.
Growth cone repulsion is an important mechanism controlling axon growth. During development it guides axons by excluding them from repulsive regions of the embryo. Following injury to the adult brain it may also block axon regeneration, with major clinical consequences. Our laboratory is investigating two axon-repulsive systems. We are characterizing somite glycoproteins that repel axons, creating the segmented pattern of spinal nerves during development. We have also purified a repulsive protein in the grey matter of the adult brain that may contribute to CNS regenerative failure. Besides elucidating the biology of these molecules and their receptors, an important clinical aim is to interfere with their inhibitory action, including the use of engineered bacterial chondroitinase, to see whether improved functional regeneration can be achieved using models of brain and spinal cord injury.
Spinal cord injury
Traumatic brain injury
Recombinant protein expression
Adrian Pini Web: http://www.kcl.ac.uk/depsta/...
Claudio Stern Web: http://sternlab.anat.ucl.ac.uk/
Muir EM, Fyfe I, Gardiner S, Li L, Warren P, Fawcett JW, Keynes RJ, Rogers JH (2010), “Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells.” J Biotechnol 145(2):103-10 Details
DST Hughes, RJ Keynes & D Tannahill (2009), “Extensive molecular differences between anterior- and posterior-half-sclerotomes underlie somite polarity and spinal nerve segmentation” BMC Developmental Biology 2009, 9:30
G Cook, J Fawcett, R Keynes & M Tessier-Lavigne (Eds) (2006), “The regenerating brain” Phil Trans Roy Soc B 361, 1459-1461
Kuan C-YK, Tannahill D, Cook GMW, Keynes RJ (2004), “Somite polarity and segmental patterning of the peripheral nervous system” Mechanisms of Development 121:1055-1068
Anderson CNG, Ohta K, Quick MM, Fleming A, Keynes R, Tannahill D (2003), “Molecular analysis of axon repulsion by the notochord” Development 130:1123-1133
Britto J, Tannahill D, Keynes R (2002), “A critical role for sonic hedgehog signaling in the early expansion of the developing brain” Nature Neuroscience 5:103-110