Professor Roger Thomas

University position

Emeritus Professor

Departments

Department of Physiology, Development and Neuroscience

Email

rct26@cam.ac.uk

Home page

http://www.pdn.cam.ac.uk/staff/t...

Research Theme

Cellular and Molecular Neuroscience

The Cambridge Neuroscience Community

Interests

Intracellular ion homeostasis in nerve cells. I use pH and Ca2+ sensitive microelectrodes to study ionic interactions inside large snail neurones. I am currently investigating the Ca:H coupling ratio of the plasma membrane Ca pump, or PMCA.

The effect of injecting HCl on the intracellular Ca of a snail neurone
Click image to view full-size

Research Focus

Keywords

Intracellular pH

intracellular Ca2+

PMCA

Clinical conditions

No direct clinical relevance

Equipment

Electrophysiological recording techniques

Intracellular recording

Ion-sensitive microelectrodes

Iontophoretic injections

Whole cell patch clamp

Collaborators

Cambridge

Christof Schwiening

Key publications

Thomas RC (2011), “The Ca(2+):H(+) coupling ratio of the plasma membrane calcium ATPase in neurones is little sensitive to changes in external or internal pH.” Cell Calcium 49(6):357-64 Details

Thomas RC (2009), “The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and exchanges 2 H+ for each Ca2+ or Ba2+ ion extruded. ” J Physiol 587.2 pp 315–327

Thomas RC, Postma M (2007), “Dynamic and static calcium gradients inside large snail (Helix aspersa) neurones detected with calcium-sensitive microelectrodes” Cell Calcium 41:365-378 Details

Thomas RC (2002), “The effects of HCl and CaCl2 injections on intracellular calcium and pH in voltage-clamped snail (Helix aspersa) neurons” J Gen Physiol 120: 567-579

Home