Dr Stefano Pluchino

Stefano Pluchino

University position

Lecturer

Dr Stefano Pluchino is pleased to consider applications from prospective PhD students.

Departments

Department of Clinical Neurosciences

Institutes

Cambridge Centre for Brain Repair and Cambridge Stem Cell Initiative

Email

spp24@cam.ac.uk

Home page

http://pluchino.brc.cam.ac.uk/labwiki... (personal home page)

Research Themes

Cellular and Molecular Neuroscience

Systems and Computational Neuroscience

Interests

Recent evidence from our own laboratory indicates that the systemic injection of somatic neural stem/precursor cells (NPCs) very efficiently protect the CNS from the chronic degeneration induced by inflammation both in small rodents as well as in non-human primates affected by experimental MS, cerebral ischemic stroke, or contusion spinal cord injury (stem cell therapeutic plasticity). We are now exploring the cellular and molecular mechanisms regulating the therapeutic plasticity of stem cells in pre-clinical models of complex CNS diseases such as multiple sclerosis, and spinal cord injury.

We are devoting special attention to the different modalities by which NPCs engage programs of horizontal cell-to-cell communication with cells in the microenvironment.

Our major aim is to look into innate (physiological) mechanisms with the visionary focus of translating the knowledge of basal (vs reactive) stem cell functions into innovative high clinical impact therapeutics.

Confocal microscopy image of two intravenously-injected β-gal+ NPCs (green) persisting (110 days after cell injection) at the level of a representative CNS perivascular area from a R-EAE mouse in very close contact to CNS-infiltrating encephalitogenic blood-borne CD45+ inflammatory cells (red) [Nature 2005; 436: 266-71].
Confocal microscopy image of two intravenously-injected β-gal+ NPCs (green) persisting (110 days after cell injection) at the level of a representative CNS perivascular area from a R-EAE mouse in very close contact to CNS-infiltrating encephalitogenic blood-borne CD45+ inflammatory cells (red) [Nature 2005; 436: 266-71].
Click image to view full-size

Research Focus

Keywords

Somatic neural stem cells

Pluripotent stem cells

Inflammation

Secreted membrane vesicles

Small regulatory RNAs

Clinical conditions

Autoimmune diseases

Cerebrovascular disorders

Epilepsy

Multiple sclerosis

Spinal cord injuries

Stroke

Traumatic brain injury

Equipment

Cell culture

Cell transplantation

Computational modelling

Confocal microscopy

Electron microscopy (scanning and transmission)

Flow cytometry

Fluorescence microscopy

hES cells

Immunohistochemistry

In situ hybridization

In vitro co-cultures

Magnetic resonance imaging (MRI)

Mathematical modeling

Microscopy

Non viral delivery technologies (nanoparticles; liposomes)

Reverse engineering

RNA deep sequencing

Semi-quantitative real time RT-PCR

siRNA

Unbiased stereology

Viral vector technology for cell engineering and ncRNA delivery

Collaborators

Cambridge

Sabine Dietmann

Robin Franklin

United Kingdom

Manolis Fanto Web: http://www.kcl.ac.uk/depsta/...

International

Angela Bachi Web: http://www.sanraffaele.org/EN_home...

Fabio Biscarini Web: http://www.bo.ismn.cnr.it/staff.p...

Michele DePalma Web: https://www.sanraffaele.org/MDePalm...

Jose-Manuel Garcia-Verdugo Web: http://www.uv.es/garciajm/

Samia Khoury Web: http://khourylab.bwh.harvard.edu/

Yang Mao-Draayer Web: http://www.med.uvm.edu/Neurolo...

John Mattick Web: http://jsm-research.imb.uq.edu.au/groupwi...

Pietro Mortini Web: http://www.mortini.net

Giovanna Musco Web: http://www.sanraffaele.org/home/ri...

Luigi Naldini Web: http://www.unisr.it/persona...

Alessandro Sannino Web: http://bioslab.unile.it

Bert t'Hart Web: http://www.bprc.nl/

Alexey Terkisk Web: http://www.burnham.org/default...

Mark West Web: http://person.au.dk/en/mjw@...

Associated News Items


    Key publications

    Cusimano M, Biziato D, Brambilla E, Donegà M, Alfaro-Cervello C, Snider S, Salani G, Pucci F, Comi G, Garcia-Verdugo JM, De Palma M, Martino G, Pluchino S (2012), “Transplanted neural stem/precursor cells instruct phagocytes and reduce secondary tissue damage in the injured spinal cord.” Brain Details

    Bacigaluppi M, Pluchino S, Peruzzotti Jametti L, Kilic E, Kilic U, Salani G, Brambilla E, West MJ, Comi G, Martino G, Hermann DM (2009), “Delayed post-ischaemic neuroprotection following systemic neural stem cell transplantation involves multiple mechanisms.” Brain 132(Pt 8):2239-51 Details

    Pluchino S, Gritti A, Blezer E, Amadio S, Brambilla E, Borsellino G, Cossetti C, Del Carro U, Comi G, 't Hart B, Vescovi A, Martino G (2009), “Human neural stem cells ameliorate autoimmune encephalomyelitis in non-human primates.” Ann Neurol 66(3):343-54 Details

    Pluchino S, Zanotti L, Brambilla E, Rovere-Querini P, Capobianco A, Alfaro-Cervello C, Salani G, Cossetti C, Borsellino G, Battistini L, Ponzoni M, Doglioni C, Garcia-Verdugo JM, Comi G, Manfredi AA, Martino G (2009), “Immune regulatory neural stem/precursor cells protect from central nervous system autoimmunity by restraining dendritic cell function.” PLoS One 4(6):e5959 Details

    Pluchino S, Muzio L, Imitola J, Deleidi M, Alfaro-Cervello C, Salani G, Porcheri C, Brambilla E, Cavasinni F, Bergamaschi A, Garcia-Verdugo JM, Comi G, Khoury SJ, Martino G (2008), “Persistent inflammation alters the function of the endogenous brain stem cell compartment.” Brain 131(Pt 10):2564-78 Details

    Pluchino S, Zanotti L, Rossi B, Brambilla E, Ottoboni L, Salani G, Martinello M, Cattalini A, Bergami A, Furlan R, Comi G, Constantin G, Martino G (2005), “Neurosphere-derived multipotent precursors promote neuroprotection by an immunomodulatory mechanism.” Nature 436(7048):266-71 Details

    Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G. (2003), “Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis.” Nature Apr 17;422(6933):688-94

    Publications

    2014

    Cerri F, Salvatore L, Memon D, Martinelli Boneschi F, Madaghiele M, Brambilla P, Del Carro U, Taveggia C, Riva N, Trimarco A, Lopez ID, Comi G, Pluchino S, Martino G, Sannino A, Quattrini A (2014), “Peripheral nerve morphogenesis induced by scaffold micropatterning.” Biomaterials 35(13):4035-45 Details

    Donegà M, Giusto E, Cossetti C, Schaeffer J, Pluchino S (2014), “Systemic injection of neural stem/progenitor cells in mice with chronic EAE.” J Vis Exp (86) Details

    L'Episcopo F, Tirolo C, Caniglia S, Testa N, Morale MC, Serapide MF, Pluchino S, Marchetti B (2014), “Targeting Wnt signaling at the neuroimmune interface for dopaminergic neuroprotection/repair in Parkinson's disease.” J Mol Cell Biol 6(1):13-26 Details

    L'Episcopo F, Tirolo C, Testa N, Caniglia S, Morale MC, Serapide MF, Pluchino S, Marchetti B (2014), “Wnt/β-Catenin Signaling Is Required to Rescue Midbrain Dopaminergic Progenitors and Promote Neurorepair in Ageing Mouse Model of Parkinson's Disease.” Stem Cells 32(8):2147-63 Details

    Peruzzotti-Jametti L, Donegá M, Giusto E, Mallucci G, Marchetti B, Pluchino S (2014), “The role of the immune system in central nervous system plasticity after acute injury.” Neuroscience Details

    Smith JA, Leonardi T, Huang B, Iraci N, Vega B, Pluchino S (2014), “Extracellular vesicles and their synthetic analogues in aging and age-associated brain diseases.” Biogerontology Details

    2013

    Drago D, Cossetti C, Iraci N, Gaude E, Musco G, Bachi A, Pluchino S (2013), “The stem cell secretome and its role in brain repair.” Biochimie 95(12):2271-85 Details

    Giusto E, Donegà M, Cossetti C, Pluchino S (2013), “Neuro-immune interactions of neural stem cell transplants: From animal disease models to human trials.” Exp Neurol Details

    Hill AF, Pegtel DM, Lambertz U, Leonardi T, O'Driscoll L, Pluchino S, Ter-Ovanesyan D, Nolte-'t Hoen EN (2013), “ISEV position paper: extracellular vesicle RNA analysis and bioinformatics.” J Extracell Vesicles 2 Details

    L'Episcopo F, Tirolo C, Testa N, Caniglia S, Morale MC, Impagnatiello F, Pluchino S, Marchetti B (2013), “Aging-induced Nrf2-ARE pathway disruption in the subventricular zone drives neurogenic impairment in parkinsonian mice via PI3K-Wnt/β-catenin dysregulation.” J Neurosci 33(4):1462-85 Details

    Marchetti B, L'Episcopo F, Morale MC, Tirolo C, Testa N, Caniglia S, Serapide MF, Pluchino S (2013), “Uncovering novel actors in astrocyte-neuron crosstalk in Parkinson's disease: the Wnt/β-catenin signaling cascade as the common final pathway for neuroprotection and self-repair.” Eur J Neurosci 37(10):1550-63 Details

    Marchetti B, Pluchino S (2013), “Wnt your brain be inflamed? Yes, it Wnt!” Trends Mol Med 19(3):144-56 Details

    Pluchino S, Cossetti C (2013), “How stem cells speak with host immune cells in inflammatory brain diseases.” Glia 61(9):1379-401 Details

    Pluchino S, Peruzzotti-Jametti L (2013), “Rewiring the ischaemic brain with human-induced pluripotent stem cell-derived cortical neurons.” Brain 136(Pt 12):3525-7 Details

    2012

    Bolton S, Hassall D, Hildebrand D, Brück W, Huang JK, Pluchino S, McKevitt T, Parry J, McFarlane M, Fagg R, Roulois A (2012), “Anti-inflammatory Disease Therapies: Challenges and Strategies in Drug Development.” Toxicol Pathol 40(1):122-5

    Cossetti C, Alfaro-Cervello C, Donegà M, Tyzack G, Pluchino S (2012), “New perspectives of tissue remodelling with neural stem and progenitor cell-based therapies.” Cell Tissue Res Details

    Cossetti C, Smith JA, Iraci N, Leonardi T, Alfaro-Cervello C, Pluchino S (2012), “Extracellular membrane vesicles and immune regulation in the brain.” Front Physiol 3:117 Details

    Herz J, Reitmeir R, Hagen SI, Reinboth BS, Guo Z, Zechariah A, Elali A, Doeppner TR, Bacigaluppi M, Pluchino S, Kilic U, Kilic E, Hermann DM (2012), “Intracerebroventricularly delivered VEGF promotes contralesional corticorubral plasticity after focal cerebral ischemia via mechanisms involving anti-inflammatory actions.” Neurobiol Dis 45(3):1077-85 Details

    Kalra H, Simpson RJ, Ji H, Aikawa E, Altevogt P, Askenase P, Bond VC, Borràs FE, Breakefield X, Budnik V, Buzas E, Camussi G, Clayton A, Cocucci E, Falcon-Perez JM, Gabrielsson S, Gho YS, Gupta D, Harsha HC, Hendrix A, Hill AF, Inal JM, Jenster G, Krämer-Albers EM, Lim SK, Llorente A, Lötvall J, Marcilla A, Mincheva-Nilsson L, Nazarenko I, Nieuwland R, Nolte-'t Hoen EN, Pandey A, Patel T, Piper MG, Pluchino S, Prasad TS, Rajendran L, Raposo G, Record M, Reid GE, Sánchez-Madrid F, Schiffelers RM, Siljander P, Stensballe A, Stoorvogel W, Taylor D, Thery C, Valadi H, van Balkom BW, Vázquez J, Vidal M, Wauben MH, Yáñez-Mó M, Zoeller M, Mathivanan S (2012), “Vesiclepedia: a compendium for extracellular vesicles with continuous community annotation.” PLoS Biol 10(12):e1001450 Details

    L'episcopo F, Tirolo C, Testa N, Caniglia S, Morale MC, Deleidi M, Serapide MF, Pluchino S, Marchetti B (2012), “Plasticity of Subventricular Zone Neuroprogenitors in MPTP (1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine) Mouse Model of Parkinson's Disease Involves Cross Talk between Inflammatory and Wnt/β-Catenin Signaling Pathways: Functional Consequences for Neuroprotection and Repair.” J Neurosci 32(6):2062-2085 Details

    2011

    Cimadamore F, Fishwick K, Giusto E, Gnedeva K, Cattarossi G, Miller A, Pluchino S, Brill LM, Bronner-Fraser M, Terskikh AV (2011), “Human ESC-derived neural crest model reveals a key role for SOX2 in sensory neurogenesis.” Cell Stem Cell 8(5):538-51 Details

    L'episcopo F, Serapide MF, Tirolo C, Testa N, Caniglia S, Morale MC, Pluchino S, Marchetti B (2011), “A Wnt1 regulated Frizzled-1/β-Catenin signaling pathway as a candidate regulatory circuit controlling mesencephalic dopaminergic neuron-astrocyte crosstalk: Therapeutical relevance for neuron survival and neuroprotection.” Mol Neurodegener 6:49 Details

    Martino G, Pluchino S, Bonfanti L, Schwartz M (2011), “Brain regeneration in physiology and pathology: the immune signature driving therapeutic plasticity of neural stem cells.” Physiol Rev 91(4):1281-304 Details

    Pluchino S, Alfaro-Cervello C (2011), “Cyclooxygenase (COX)-1 takes control of adult hippocampal neurogenesis.” Cell Cycle 10(19):3229 Details

    Reitmeir R, Kilic E, Kilic U, Bacigaluppi M, ElAli A, Salani G, Pluchino S, Gassmann M, Hermann DM (2011), “Post-acute delivery of erythropoietin induces stroke recovery by promoting perilesional tissue remodelling and contralesional pyramidal tract plasticity.” Brain 134(Pt 1):84-99 Details

    2010

    Freedman MS, Bar-Or A, Atkins HL, Karussis D, Frassoni F, Lazarus H, Scolding N, Slavin S, Le Blanc K, Uccelli A, MSCT Study Group (2010), “The therapeutic potential of mesenchymal stem cell transplantation as a treatment for multiple sclerosis: consensus report of the International MSCT Study Group.” Mult Scler 16(4):503-10 Details

    2009

    Gagliano N, Costa F, Cossetti C, Pettinari L, Bassi R, Chiriva-Internati M, Cobos E, Gioia M, Pluchino S (2009), “Glioma-astrocyte interaction modifies the astrocyte phenotype in a co-culture experimental model.” Oncol Rep 22(6):1349-56 Details

    Pluchino S, Zanotti L, Brini E, Ferrari S, Martino G (2009), “Regeneration and repair in multiple sclerosis: the role of cell transplantation.” Neurosci Lett 456(3):101-6 Details

    Shechter R, London A, Varol C, Raposo C, Cusimano M, Yovel G, Rolls A, Mack M, Pluchino S, Martino G, Jung S, Schwartz M (2009), “Infiltrating blood-derived macrophages are vital cells playing an anti-inflammatory role in recovery from spinal cord injury in mice.” PLoS Med 6(7):e1000113 Details

    2008

    Bacigaluppi M, Pluchino S, Martino G, Kilic E, Hermann DM (2008), “Neural stem/precursor cells for the treatment of ischemic stroke.” J Neurol Sci 265(1-2):73-7 Details

    Butti E, Bergami A, Recchia A, Brambilla E, Del Carro U, Amadio S, Cattalini A, Esposito M, Stornaiuolo A, Comi G, Pluchino S, Mavilio F, Martino G, Furlan R (2008), “IL4 gene delivery to the CNS recruits regulatory T cells and induces clinical recovery in mouse models of multiple sclerosis.” Gene Ther 15(7):504-15 Details

    Pluchino S, Martino G (2008), “Neural stem cell-mediated immunomodulation: repairing the haemorrhagic brain.” Brain 131(Pt 3):604-5 Details

    Pluchino S, Martino G (2008), “The therapeutic plasticity of neural stem/precursor cells in multiple sclerosis.” J Neurol Sci 265(1-2):105-10 Details

    Serra PA, Pluchino S, Marchetti B, Desole MS, Miele E (2008), “The MPTP mouse model: cues on DA release and neural stem cell restorative role.” Parkinsonism Relat Disord 14 Suppl 2:S189-93 Details

    2007

    Martino G, Pluchino S (2007), “Neural stem cells: guardians of the brain.” Nat Cell Biol 9(9):1031-4 Details

    2006

    Amadio S, Pluchino S, Brini E, Morana P, Guerriero R, Boneschi FM, Comi G, Zaratin P, Muzio V, del Carro U (2006), “Motor evoked potentials in a mouse model of chronic multiple sclerosis.” Muscle Nerve 33(2):265-73 Details

    Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M (2006), “Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.” Mol Cell Neurosci 31(1):149-60 Details

    Capriles E, Do Campo A, Verde O, Pluchino S, Capriles Hulett A (2006), “Children's asthma and the third world: an approach.” J Investig Allergol Clin Immunol 16(1):11-8 Details

    Martino G, Pluchino S (2006), “The therapeutic potential of neural stem cells.” Nat Rev Neurosci 7(5):395-406 Details

    Ziv Y, Avidan H, Pluchino S, Martino G, Schwartz M (2006), “Synergy between immune cells and adult neural stem/progenitor cells promotes functional recovery from spinal cord injury.” Proc Natl Acad Sci U S A 103(35):13174-9 Details

    2005

    Pluchino S, Bacigaluppi M, Bucello S, Butti E, Deleidi M, Zanotti L, Martino G, Furlan R (2005), “Gene and stem cell therapy for autoimmune demyelination.” Ernst Schering Res Found Workshop (53):133-46 Details

    Pluchino S, Martino G (2005), “The therapeutic use of stem cells for myelin repair in autoimmune demyelinating disorders.” J Neurol Sci 233(1-2):117-9 Details

    Pluchino S, Zanotti L, Deleidi M, Martino G (2005), “Neural stem cells and their use as therapeutic tool in neurological disorders.” Brain Res Brain Res Rev 48(2):211-9 Details

    2004

    Furlan R, Butti E, Pluchino S, Martino G (2004), “Gene therapy for autoimmune diseases.” Curr Opin Mol Ther 6(5):525-36 Details

    2003

    Furlan R, Brambilla E, Sanvito F, Roccatagliata L, Olivieri S, Bergami A, Pluchino S, Uccelli A, Comi G, Martino G (2003), “Vaccination with amyloid-beta peptide induces autoimmune encephalomyelitis in C57/BL6 mice.” Brain 126(Pt 2):285-91 Details

    Furlan R, Pluchino S, Marconi PC, Martino G (2003), “Cytokine gene delivery into the central nervous system using intrathecally injected nonreplicative viral vectors.” Methods Mol Biol 215:279-89 Details

    Furlan R, Pluchino S, Martino G (2003), “Gene therapy-mediated modulation of immune processes in the central nervous system.” Curr Pharm Des 9(24):2002-8 Details

    Furlan R, Pluchino S, Martino G (2003), “The therapeutic use of gene therapy in inflammatory demyelinating diseases of the central nervous system.” Curr Opin Neurol 16(3):385-92 Details

    Hernández Muñoz G, Pluchino S (2003), “Cimicifuga racemosa for the treatment of hot flushes in women surviving breast cancer.” Maturitas 44 Suppl 1:S59-65 Details

    Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G (2003), “Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis.” Nature 422(6933):688-94 Details

    Pluchino S, Zanotti L, Martino G (2003), “Antibodies and myelination: facts and misacts.” Neurol Sci 24 Suppl 4:S231-3 Details

    1997

    Annunziata P, Pluchino S, Martino T, Guazzi G (1997), “High levels of cerebrospinal fluid IgM binding to myelin basic protein are associated with early benign course in multiple sclerosis.” J Neuroimmunol 77(1):128-33 Details