Dr Stefano Pluchino

Stefano Pluchino

University position

Lecturer

Dr Stefano Pluchino is pleased to consider applications from prospective PhD students.

Departments

Department of Clinical Neurosciences

Institutes

Cambridge Centre for Brain Repair and Cambridge Stem Cell Initiative

Email

spp24@cam.ac.uk

Home page

http://pluchino.brc.cam.ac.uk/labwiki... (personal home page)

Research Themes

Cellular and Molecular Neuroscience

Systems and Computational Neuroscience

Interests

Recent evidence from our own laboratory indicates that the systemic injection of somatic neural stem/precursor cells (NPCs) very efficiently protect the CNS from the chronic degeneration induced by inflammation both in small rodents as well as in non-human primates affected by experimental MS, cerebral ischemic stroke, or contusion spinal cord injury (stem cell therapeutic plasticity). We are now exploring the cellular and molecular mechanisms regulating the therapeutic plasticity of stem cells in pre-clinical models of complex CNS diseases such as multiple sclerosis, and spinal cord injury.

We are devoting special attention to the different modalities by which NPCs engage programs of horizontal cell-to-cell communication with cells in the microenvironment.

Our major aim is to look into innate (physiological) mechanisms with the visionary focus of translating the knowledge of basal (vs reactive) stem cell functions into innovative high clinical impact therapeutics.

Confocal microscopy image of two intravenously-injected β-gal+ NPCs (green) persisting (110 days after cell injection) at the level of a representative CNS perivascular area from a R-EAE mouse in very close contact to CNS-infiltrating encephalitogenic blood-borne CD45+ inflammatory cells (red) [Nature 2005; 436: 266-71].
Confocal microscopy image of two intravenously-injected β-gal+ NPCs (green) persisting (110 days after cell injection) at the level of a representative CNS perivascular area from a R-EAE mouse in very close contact to CNS-infiltrating encephalitogenic blood-borne CD45+ inflammatory cells (red) [Nature 2005; 436: 266-71].
Click image to view full-size

Research Focus

Keywords

Somatic neural stem cells

Pluripotent stem cells

Inflammation

Secreted membrane vesicles

Small regulatory RNAs

Clinical conditions

Autoimmune diseases

Cerebrovascular disorders

Epilepsy

Multiple sclerosis

Spinal cord injuries

Stroke

Traumatic brain injury

Equipment

Cell culture

Cell transplantation

Computational modelling

Confocal microscopy

Electron microscopy (scanning and transmission)

Flow cytometry

Fluorescence microscopy

hES cells

Immunohistochemistry

In situ hybridization

In vitro co-cultures

Magnetic resonance imaging (MRI)

Mathematical modeling

Microscopy

Non viral delivery technologies (nanoparticles; liposomes)

Reverse engineering

RNA deep sequencing

Semi-quantitative real time RT-PCR

siRNA

Unbiased stereology

Viral vector technology for cell engineering and ncRNA delivery

Collaborators

Cambridge

Sabine Dietmann

Robin Franklin

United Kingdom

Manolis Fanto Web: http://www.kcl.ac.uk/depsta/...

International

Angela Bachi Web: http://www.sanraffaele.org/EN_home...

Fabio Biscarini Web: http://www.bo.ismn.cnr.it/staff.p...

Michele DePalma Web: https://www.sanraffaele.org/MDePalm...

Jose-Manuel Garcia-Verdugo Web: http://www.uv.es/garciajm/

Samia Khoury Web: http://khourylab.bwh.harvard.edu/

Yang Mao-Draayer Web: http://www.med.uvm.edu/Neurolo...

John Mattick Web: http://jsm-research.imb.uq.edu.au/groupwi...

Pietro Mortini Web: http://www.mortini.net

Giovanna Musco Web: http://www.sanraffaele.org/home/ri...

Luigi Naldini Web: http://www.unisr.it/persona...

Alessandro Sannino Web: http://bioslab.unile.it

Bert t'Hart Web: http://www.bprc.nl/

Alexey Terkisk Web: http://www.burnham.org/default...

Mark West Web: http://person.au.dk/en/mjw@...

Key publications

Cusimano M, Biziato D, Brambilla E, Donegà M, Alfaro-Cervello C, Snider S, Salani G, Pucci F, Comi G, Garcia-Verdugo JM, De Palma M, Martino G, Pluchino S (2012), “Transplanted neural stem/precursor cells instruct phagocytes and reduce secondary tissue damage in the injured spinal cord.” Brain Details

Bacigaluppi M, Pluchino S, Peruzzotti Jametti L, Kilic E, Kilic U, Salani G, Brambilla E, West MJ, Comi G, Martino G, Hermann DM (2009), “Delayed post-ischaemic neuroprotection following systemic neural stem cell transplantation involves multiple mechanisms.” Brain 132(Pt 8):2239-51 Details

Pluchino S, Gritti A, Blezer E, Amadio S, Brambilla E, Borsellino G, Cossetti C, Del Carro U, Comi G, 't Hart B, Vescovi A, Martino G (2009), “Human neural stem cells ameliorate autoimmune encephalomyelitis in non-human primates.” Ann Neurol 66(3):343-54 Details

Pluchino S, Zanotti L, Brambilla E, Rovere-Querini P, Capobianco A, Alfaro-Cervello C, Salani G, Cossetti C, Borsellino G, Battistini L, Ponzoni M, Doglioni C, Garcia-Verdugo JM, Comi G, Manfredi AA, Martino G (2009), “Immune regulatory neural stem/precursor cells protect from central nervous system autoimmunity by restraining dendritic cell function.” PLoS One 4(6):e5959 Details

Pluchino S, Muzio L, Imitola J, Deleidi M, Alfaro-Cervello C, Salani G, Porcheri C, Brambilla E, Cavasinni F, Bergamaschi A, Garcia-Verdugo JM, Comi G, Khoury SJ, Martino G (2008), “Persistent inflammation alters the function of the endogenous brain stem cell compartment.” Brain 131(Pt 10):2564-78 Details

Pluchino S, Zanotti L, Rossi B, Brambilla E, Ottoboni L, Salani G, Martinello M, Cattalini A, Bergami A, Furlan R, Comi G, Constantin G, Martino G (2005), “Neurosphere-derived multipotent precursors promote neuroprotection by an immunomodulatory mechanism.” Nature 436(7048):266-71 Details

Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G. (2003), “Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis.” Nature Apr 17;422(6933):688-94

Publications

2012

Bolton S, Hassall D, Hildebrand D, Brück W, Huang JK, Pluchino S, McKevitt T, Parry J, McFarlane M, Fagg R, Roulois A (2012), “Anti-inflammatory Disease Therapies: Challenges and Strategies in Drug Development.” Toxicol Pathol 40(1):122-5

Cossetti C, Alfaro-Cervello C, Donegà M, Tyzack G, Pluchino S (2012), “New perspectives of tissue remodelling with neural stem and progenitor cell-based therapies.” Cell Tissue Res Details

Herz J, Reitmeir R, Hagen SI, Reinboth BS, Guo Z, Zechariah A, Elali A, Doeppner TR, Bacigaluppi M, Pluchino S, Kilic U, Kilic E, Hermann DM (2012), “Intracerebroventricularly delivered VEGF promotes contralesional corticorubral plasticity after focal cerebral ischemia via mechanisms involving anti-inflammatory actions.” Neurobiol Dis 45(3):1077-85 Details

L'episcopo F, Tirolo C, Testa N, Caniglia S, Morale MC, Deleidi M, Serapide MF, Pluchino S, Marchetti B (2012), “Plasticity of Subventricular Zone Neuroprogenitors in MPTP (1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine) Mouse Model of Parkinson's Disease Involves Cross Talk between Inflammatory and Wnt/β-Catenin Signaling Pathways: Functional Consequences for Neuroprotection and Repair.” J Neurosci 32(6):2062-2085 Details

2011

Cimadamore F, Fishwick K, Giusto E, Gnedeva K, Cattarossi G, Miller A, Pluchino S, Brill LM, Bronner-Fraser M, Terskikh AV (2011), “Human ESC-derived neural crest model reveals a key role for SOX2 in sensory neurogenesis.” Cell Stem Cell 8(5):538-51 Details

L'episcopo F, Serapide MF, Tirolo C, Testa N, Caniglia S, Morale MC, Pluchino S, Marchetti B (2011), “A Wnt1 regulated Frizzled-1/β-Catenin signaling pathway as a candidate regulatory circuit controlling mesencephalic dopaminergic neuron-astrocyte crosstalk: Therapeutical relevance for neuron survival and neuroprotection.” Mol Neurodegener 6:49 Details

Martino G, Pluchino S, Bonfanti L, Schwartz M (2011), “Brain regeneration in physiology and pathology: the immune signature driving therapeutic plasticity of neural stem cells.” Physiol Rev 91(4):1281-304 Details

Pluchino S, Alfaro-Cervello C (2011), “Cyclooxygenase (COX)-1 takes control of adult hippocampal neurogenesis.” Cell Cycle 10(19):3229 Details

Reitmeir R, Kilic E, Kilic U, Bacigaluppi M, ElAli A, Salani G, Pluchino S, Gassmann M, Hermann DM (2011), “Post-acute delivery of erythropoietin induces stroke recovery by promoting perilesional tissue remodelling and contralesional pyramidal tract plasticity.” Brain 134(Pt 1):84-99 Details

2010

Freedman MS, Bar-Or A, Atkins HL, Karussis D, Frassoni F, Lazarus H, Scolding N, Slavin S, Le Blanc K, Uccelli A, MSCT Study Group (2010), “The therapeutic potential of mesenchymal stem cell transplantation as a treatment for multiple sclerosis: consensus report of the International MSCT Study Group.” Mult Scler 16(4):503-10 Details

2009

Gagliano N, Costa F, Cossetti C, Pettinari L, Bassi R, Chiriva-Internati M, Cobos E, Gioia M, Pluchino S (2009), “Glioma-astrocyte interaction modifies the astrocyte phenotype in a co-culture experimental model.” Oncol Rep 22(6):1349-56 Details

Pluchino S, Zanotti L, Brini E, Ferrari S, Martino G (2009), “Regeneration and repair in multiple sclerosis: the role of cell transplantation.” Neurosci Lett 456(3):101-6 Details

Shechter R, London A, Varol C, Raposo C, Cusimano M, Yovel G, Rolls A, Mack M, Pluchino S, Martino G, Jung S, Schwartz M (2009), “Infiltrating blood-derived macrophages are vital cells playing an anti-inflammatory role in recovery from spinal cord injury in mice.” PLoS Med 6(7):e1000113 Details

2008

Bacigaluppi M, Pluchino S, Martino G, Kilic E, Hermann DM (2008), “Neural stem/precursor cells for the treatment of ischemic stroke.” J Neurol Sci 265(1-2):73-7 Details

Butti E, Bergami A, Recchia A, Brambilla E, Del Carro U, Amadio S, Cattalini A, Esposito M, Stornaiuolo A, Comi G, Pluchino S, Mavilio F, Martino G, Furlan R (2008), “IL4 gene delivery to the CNS recruits regulatory T cells and induces clinical recovery in mouse models of multiple sclerosis.” Gene Ther 15(7):504-15 Details

Pluchino S, Martino G (2008), “Neural stem cell-mediated immunomodulation: repairing the haemorrhagic brain.” Brain 131(Pt 3):604-5 Details

Pluchino S, Martino G (2008), “The therapeutic plasticity of neural stem/precursor cells in multiple sclerosis.” J Neurol Sci 265(1-2):105-10 Details

Serra PA, Pluchino S, Marchetti B, Desole MS, Miele E (2008), “The MPTP mouse model: cues on DA release and neural stem cell restorative role.” Parkinsonism Relat Disord 14 Suppl 2:S189-93 Details

2007

Martino G, Pluchino S (2007), “Neural stem cells: guardians of the brain.” Nat Cell Biol 9(9):1031-4 Details

2006

Amadio S, Pluchino S, Brini E, Morana P, Guerriero R, Boneschi FM, Comi G, Zaratin P, Muzio V, del Carro U (2006), “Motor evoked potentials in a mouse model of chronic multiple sclerosis.” Muscle Nerve 33(2):265-73 Details

Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M (2006), “Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.” Mol Cell Neurosci 31(1):149-60 Details

Martino G, Pluchino S (2006), “The therapeutic potential of neural stem cells.” Nat Rev Neurosci 7(5):395-406 Details

Ziv Y, Avidan H, Pluchino S, Martino G, Schwartz M (2006), “Synergy between immune cells and adult neural stem/progenitor cells promotes functional recovery from spinal cord injury.” Proc Natl Acad Sci U S A 103(35):13174-9 Details

2005

Pluchino S, Bacigaluppi M, Bucello S, Butti E, Deleidi M, Zanotti L, Martino G, Furlan R (2005), “Gene and stem cell therapy for autoimmune demyelination.” Ernst Schering Res Found Workshop (53):133-46 Details

Pluchino S, Martino G (2005), “The therapeutic use of stem cells for myelin repair in autoimmune demyelinating disorders.” J Neurol Sci 233(1-2):117-9 Details

Pluchino S, Zanotti L, Deleidi M, Martino G (2005), “Neural stem cells and their use as therapeutic tool in neurological disorders.” Brain Res Brain Res Rev 48(2):211-9 Details

2004

Furlan R, Butti E, Pluchino S, Martino G (2004), “Gene therapy for autoimmune diseases.” Curr Opin Mol Ther 6(5):525-36 Details

2003

Furlan R, Brambilla E, Sanvito F, Roccatagliata L, Olivieri S, Bergami A, Pluchino S, Uccelli A, Comi G, Martino G (2003), “Vaccination with amyloid-beta peptide induces autoimmune encephalomyelitis in C57/BL6 mice.” Brain 126(Pt 2):285-91 Details

Furlan R, Pluchino S, Marconi PC, Martino G (2003), “Cytokine gene delivery into the central nervous system using intrathecally injected nonreplicative viral vectors.” Methods Mol Biol 215:279-89 Details

Furlan R, Pluchino S, Martino G (2003), “Gene therapy-mediated modulation of immune processes in the central nervous system.” Curr Pharm Des 9(24):2002-8 Details

Furlan R, Pluchino S, Martino G (2003), “The therapeutic use of gene therapy in inflammatory demyelinating diseases of the central nervous system.” Curr Opin Neurol 16(3):385-92 Details

Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G (2003), “Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis.” Nature 422(6933):688-94 Details

Pluchino S, Zanotti L, Martino G (2003), “Antibodies and myelination: facts and misacts.” Neurol Sci 24 Suppl 4:S231-3 Details

1997

Annunziata P, Pluchino S, Martino T, Guazzi G (1997), “High levels of cerebrospinal fluid IgM binding to myelin basic protein are associated with early benign course in multiple sclerosis.” J Neuroimmunol 77(1):128-33 Details