Dr Matthias Landgraf

Matthias Landgraf

University position

Lecturer

Dr Matthias Landgraf is pleased to consider applications from prospective PhD students.

Departments

Department of Zoology

Email

ml10006@cam.ac.uk

Home page

http://www.zoo.cam.ac.uk/departm... (personal home page)

Research Themes

Developmental Neuroscience

Cellular and Molecular Neuroscience

Interests

Development of neural networks - from the morphogenesis of dendritic trees to patterns of connections.

As neuronal circuits form, synaptic terminals are delivered to specific regions of the nervous system so that connections can form between appropriate partner neurons. We investigate the genetic and cellular mechanisms that underlie this process. To this end we have generated a battery of genetic tools that allow us to differentially manipulate, visualise and image identified partner neurons as the network forms and develops. Using these methods we apply genetics to study the mechanisms that regulate the targeting, growth and branching of synaptic terminal arbors, and how this interlinks with the formation and adjustment of synapses.

Linking our work to the clinically relevant, we find that reactive oxygen species, which are elevated with ageing and in neurodegenerative conditions, are part of elementary signalling pathways that regulates synaptic terminal growth and adjustment.

The image shows sets of motorneurons and their dendritic territories in the Drosophila embryonic nerve cord (left and right). Dendrites are targeted to distinct antero-posterior (left) and medio-lateral domains (right), which reflect the distribution of their muscle targets in the periphery. The diagram in the centre shows a half segment of muscles composed of internal (cyan, green, red) and external muscles (yellow).
The image shows sets of motorneurons and their dendritic territories in the Drosophila embryonic nerve cord (left and right). Dendrites are targeted to distinct antero-posterior (left) and medio-lateral domains (right), which reflect the distribution of their muscle targets in the periphery. The diagram in the centre shows a half segment of muscles composed of internal (cyan, green, red) and external muscles (yellow).
Click image to view full-size

Research Focus

Keywords

Drosophila

development

homeostasis

reactive oxygen species

synapse

Clinical conditions

Alzheimer's disease

Mental Retardation

Parkinson's disease

Prion diseases

Prion diseases

Equipment

Cell labelling

Confocal microscopy

Electrophysiological recording techniques

Fluorescence microscopy

Fluorescence microscopy

Genetic intersectional strategies

Immunocytochemistry

Live imaging

Collaborators

Cambridge

Michael Bate

Raymond Bujdoso

Gregory Jefferis

Kevin O'Holleran

Matthew Wayland

United Kingdom

Richard Baines Web: http://www.ls.manchester.ac.uk/people/...

Sangeeta Chawla Web: https://www.york.ac.uk/biology...

Richard Ribchester Web: http://www.ed.ac.uk/integra...

Sean Sweeney Web: http://www.york.ac.uk/biology...

International

Albert Cardona Web: http://www.hhmi.org/scienti...

Haluk Lacin Web: https://www.janelia.org/people/...

Gerd Technau Web: http://www.uni-mainz.de/FB/Biol...

Jim Truman Web: https://www.janelia.org/lab/tru...

Don van Meyel Web: http://www.mcgill.ca/crn/inv...

Benjamin White Web: http://neuroscience.nih.gov/Lab.asp...

Marta Zlatic Web: https://www.janelia.org/lab/zla...

Associated News Items


Publications

2013

Singh AP, Das RN, Rao G, Aggarwal A, Diegelmann S, Evers JF, Karandikar H, Landgraf M, Rodrigues V, Vijayraghavan K (2013), “Sensory neuron-derived eph regulates glomerular arbors and modulatory function of a central serotonergic neuron.” PLoS Genet 9(4):e1003452 Details

Zwart MF, Randlett O, Evers JF, Landgraf M (2013), “Dendritic growth gated by a steroid hormone receptor underlies increases in activity in the developing Drosophila locomotor system.” Proc Natl Acad Sci U S A 110(40):E3878-87 Details

2012

Thackray AM, Muhammad F, Zhang C, Di Y, Jahn TR, Landgraf M, Crowther DC, Evers JF, Bujdoso R (2012), “Ovine PrP transgenic Drosophila show reduced locomotor activity and decreased survival.” Biochem J 444(3):487-95 Details

2010

Nicola├» LJ, Ramaekers A, Raemaekers T, Drozdzecki A, Mauss AS, Yan J, Landgraf M, Annaert W, Hassan BA (2010), “Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila.” Proc Natl Acad Sci U S A 107(47):20553-8 Details

2009

Mauss A, Tripodi M, Evers JF, Landgraf M (2009), “Midline signalling systems direct the formation of a neural map by dendritic targeting in the Drosophila motor system.” PLoS Biol 7(9):e1000200 Details

2008

Diegelmann S, Bate M, Landgraf M (2008), “Gateway cloning vectors for the LexA-based binary expression system in Drosophila.” Fly (Austin) 2(4):236-9 Details

Ou Y, Chwalla B, Landgraf M, van Meyel DJ (2008), “Identification of genes influencing dendrite morphogenesis in developing peripheral sensory and central motor neurons.” Neural Dev 3:16 Details

Tripodi M, Evers JF, Mauss A, Bate M, Landgraf M (2008), “Structural homeostasis: compensatory adjustments of dendritic arbor geometry in response to variations of synaptic input.” PLoS Biol 6(10):e260 Details

2006

Landgraf M, Thor S (2006), “Development and structure of motoneurons.” Int Rev Neurobiol 75:33-53 Details

Landgraf M, Thor S (2006), “Development of Drosophila motoneurons: specification and morphology.” Semin Cell Dev Biol 17(1):3-11 Details

2005

Landgraf M, Evers JF (2005), “Control of dendritic diversity.” Curr Opin Cell Biol 17(6):690-6 Details

2003

Landgraf M, Jeffrey V, Fujioka M, Jaynes JB, Bate M (2003), “Embryonic origins of a motor system: motor dendrites form a myotopic map in Drosophila.” PLoS Biol 1(2):E41 Details

Landgraf M, S├ínchez-Soriano N, Technau GM, Urban J, Prokop A (2003), “Charting the Drosophila neuropile: a strategy for the standardised characterisation of genetically amenable neurites.” Dev Biol 260(1):207-25 Details

Zlatic M, Landgraf M, Bate M (2003), “Genetic specification of axonal arbors: atonal regulates robo3 to position terminal branches in the Drosophila nervous system.” Neuron 37(1):41-51 Details