Dr Jochen Guck

Jochen Guck

University position

Reader

Dr Jochen Guck is pleased to consider applications from prospective PhD students.

Departments

Department of Physics

Institutes

Physics of Medicine

Email

jg473@cam.ac.uk

Home page

http://www.bss.phy.cam.ac.uk/~jg473/ (personal home page)

Research Themes

Cellular and Molecular Neuroscience

Developmental Neuroscience

Interests

The paradigm that neurons in the CNS cannot regenerate is gone. While most research to date is biochemical, there are also physical aspects that need to be considered. We are developing tools to investigate axonal growth and to direct in a certain direction using physical stimuli. We are also investigating the importance of mechanical cues for normal differentiation and axonal pathfinding during development and after trauma. Specifically, we want to find out whether glial scars present after injury pose mechanical barriers to nerve regeneration. More generally, we are interested in the mechanical and optical properties of living cells and tissues using novel photonic tools to test their relevance and importance for biological function. Towards this end we could show that the deformability of cells can be used as a sensitive inherent marker for cancer diagnosis and sorting of stem cells and that glial cells in the retina act as living optical light guides (see image below).

Müller cells, the glial cells of the retina, function as living optical fibers and channel the light through the inverted vertebrate retina to the photoreceptor cells at the far side.
Müller cells, the glial cells of the retina, function as living optical fibers and channel the light through the inverted vertebrate retina to the photoreceptor cells at the far side.
Click image to view full-size

Research Focus

Keywords

biophysics

biophotonics

biomechanics

neuromechanics

mechanosensing

Clinical conditions

Cancers

Spinal cord injury

Traumatic brain injury

Equipment

Atomic force microscopy

Calcium imaging

Cell culture

Confocal microscopy

Fluorescence microscopy

Immunohistochemistry

Microscopy

Optical stretcher

Optical traps

Collaborators

Cambridge

James Fawcett

Robin Franklin

Bill Harris

Christine Holt

Ragnhildur Karadottir

Stephanie Lacour

International

Andreas Reichenbach Web: http://www.uni-leipzig.de/~pfi/pf...

Key publications

Lautenschläger F, Paschke S, Schinkinger S, Bruel A, Beil M, Guck J (2009), “The regulatory role of cell mechanics for migration of differentiating myeloid cells.” Proc Natl Acad Sci U S A 106(37):15696-701 Details

Solovei I, Kreysing M, Lanctôt C, Kösem S, Peichl L, Cremer T, Guck J, Joffe B (2009), “Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution.” Cell 137(2):356-68 Details

Franze K, Grosche J, Skatchkov SN, Schinkinger S, Schild D, Uckermann O, Travis K, Reichenbach A, Guck J (2007), “Müller Cells are Living Optical Fibers in the Vertebrate Retina” Proc Natl Acad Sci USA 104(20):8287-8292 Details

Lu Y, Franze K, Seifert G, Steinhäuser Ch, Kirchhoff F, Wolburg H, Guck J, Janmey P, Wei E, Käs J, Reichenbach A (2006), “Viscoelastic properties of individual glial cells and neurons in the CNS” Proc Natl Acad Sci 103(47):17759-177764