Dr Ingo Greger

Ingo Greger

University position

Group leader

Dr Ingo Greger is pleased to consider applications from prospective PhD students.

Institutes

Medical Research Council Laboratory of Molecular Biology

Email

ig@mrc-lmb.cam.ac.uk

Home page

http://www2.mrc-lmb.cam.ac.uk/group-l...

Research Theme

Cellular and Molecular Neuroscience

Interests

Information transfer in the brain occurs at synapses where chemical transmitters are released from presynaptic terminals and are received by postsynaptic receptors. Glutamate is a major neurotransmitter and glutamate receptors are key to synaptic transmission and plasticity. Our research focuses on these receptors, in particular on the AMPA-type (AMPAR).

AMPARs are the first to respond to glutamate and initiate synaptic signaling by depolarizing the postsynaptic membrane. AMPARs are unique as their rapid kinetics permit faithful decoding of high-frequency nerve impulses. Moreover, AMPAR are mobile and their trafficking is a major determinant of synaptic plasticity.

Our ultimate aim is to understand how regulation of these receptors underlies learning and information storage at synapses. Towards this goal we are utilizing a variety of experimental approaches at different levels of complexity, ranging from the atomic structure of the receptor to its operation in neural networks.

Research Focus

Keywords

AMPA receptor

trafficking

RNA editing

synaptic plasticity

ligand design

Clinical conditions

ALS

Alzheimer's disease

Autoimmune diseases

Dementia

Epilepsy

Equipment

cryo electron microscopy (cryo-EM)

Electrophysiological recording techniques

Field potential recording

Fluorescence microscopy

Molecular biology

Protein purification

superresolution microscopy

Whole cell patch clamp

X-ray crystallography

Collaborators

Cambridge

Ole Paulsen

United Kingdom

A. Radu Aricescu Web: https://www.strubi.ox.ac.uk/researc...

Stuart Cull-Candy Web: http://www.ucl.ac.uk/npp/res...

Jernej Ule Web: http://ulelab.info/

International

Ivet Bahar Web: http://www.ccbb.pitt.edu/Faculty...

Terunaga Nakagawa Web: http://www.mc.vanderbilt.edu/root/vu...

Associated News Items


Key publications

Herguedas B, Garcia-Nafria J, Cais O, Fernandez-Leiro R, Krieger J, Ho Ho, Greger IH. (2016), “Structure and organization of heteromeric AMPA-type glutamate receptors” Science doi: 10.1126/Science.aad3873

Publications

2017

Greger IH, Watson JF, Cull-Candy S (2017), “Structural and functional architecture of AMPA-type glutamate receptors and their auxiliary proteins” Neuron 94:713-730

Watson JF, Ho H, Greger IH (2017), “Synaptic transmission and plasticity require AMPA receptor anchoring via its N-terminal domain” eLife

2015

Dutta A, Krieger J, Lee JY, Garcia-Nafria J, Greger IH, Bahar I (2015), “Cooperative Dynamics of Intact AMPA and NMDA Glutamate Receptors: Similarities and Subfamily-Specific Differences” Structure

in press

Garcia-Nafria J, Hergueda B, Watson J, Greger IH (in press), “Structural dynamics of synaptic AMPA receptors” J. Physiol.

2015

Krieger J, Bahar I, Greger IH (2015), “Structure, Dynamics, and Allosteric Potential of Ionotropic Glutamate Receptor N-Terminal Domains” Biophys. J

2014

Cais O, Herguedas B, Krol K, Cull-Candy SG, Farrant M, Greger IH (2014), “Mapping the interaction sites between AMPA receptors and TARPs reveals a role for the receptor N-terminal domain in channel gating” Cell Reports 9, 728-40. PDF

Shanks NF, Cais O, Maruo T, Savas JN, Zaika EI, Azumaya CM, Yates JR 3rd, Greger I, Nakagawa T (2014), “Molecular dissection of the interaction between the AMPA receptor and cornichon homolog-3.” J Neurosci 34(36):12104-20 Details

2013

Balik A, Penn AC, Nemoda Z, Greger IH (2013), “Activity-regulated RNA editing in select neuronal subfields in hippocampus.” Nucleic Acids Res 41(2):1124-34 Details

Herguedas B, Krieger J, Greger IH (2013), “Receptor heteromeric assembly-how it works and why it matters: the case of ionotropic glutamate receptors.” Prog Mol Biol Transl Sci 117:361-86 PDF Details

Penn AC, Balik A, Greger IH (2013), “Reciprocal regulation of A-to-I RNA editing and the vertebrate nervous system.” Front Neurosci 7:61 Details

Penn AC, Balik A, Greger IH (2013), “Steric antisense inhibition of AMPA receptor Q/R editing reveals tight coupling to intronic editing sites and splicing.” Nucleic Acids Res 41(2):1113-23 Details

2012

Dutta A, Shrivastava IH, Sukumaran M, Greger IH, Bahar I (2012), “Comparative dynamics of NMDA- and AMPA-glutamate receptor N-terminal domains.” Structure 20(11):1838-49 Details

Penn AC, Balik A, Wozny C, Cais O, Greger IH (2012), “Activity-mediated AMPA receptor remodeling, driven by alternative splicing in the ligand-binding domain.” Neuron 76(3):503-10 Details

Shanks NF, Savas JN, Maruo T, Cais O, Hirao A, Oe S, Ghosh A, Noda Y, Greger IH, Yates JR 3rd, Nakagawa T (2012), “Differences in AMPA and kainate receptor interactomes facilitate identification of AMPA receptor auxiliary subunit GSG1L.” Cell Rep 1(6):590-8 Details

Sukumaran M, Penn AC, Greger IH (2012), “AMPA receptor assembly: atomic determinants and built-in modulators.” Adv Exp Med Biol 970:241-64 PDF Details

2011

Jensen MH, Sukumaran M, Johnson CM, Greger IH, Neuweiler H (2011), “Intrinsic motions in the N-terminal domain of an ionotropic glutamate receptor detected by fluorescence correlation spectroscopy.” J Mol Biol 414(1):96-105 Details

Rossmann M, Sukumaran M, Penn AC, Veprintsev DB, Babu MM, Greger IH (2011), “Subunit-selective N-terminal domain associations organize the formation of AMPA receptor heteromers.” EMBO J 30(5):959-71 Details

Sukumaran M, Rossmann M, Shrivastava I, Dutta A, Bahar I, Greger IH (2011), “Dynamics and allosteric potential of the AMPA receptor N-terminal domain.” EMBO J 30(5):972-82 Details

2009

Penn AC, Greger IH (2009), “Sculpting AMPA receptor formation and function by alternative RNA processing.” RNA Biol 6(5):517-21 PDF Details

2008

Penn AC, Williams SR, Greger IH (2008), “Gating motions underlie AMPA receptor secretion from the endoplasmic reticulum.” EMBO J 27(22):3056-68 Details

2007

Greger IH, Esteban JA (2007), “AMPA receptor biogenesis and trafficking.” Curr Opin Neurobiol 17(3):289-97 Details

Greger IH, Ziff EB, Penn AC (2007), “Molecular determinants of AMPA receptor subunit assembly.” Trends Neurosci 30(8):407-16 PDF Details

2006

Greger IH, Akamine P, Khatri L, Ziff EB (2006), “Developmentally regulated, combinatorial RNA processing modulates AMPA receptor biogenesis.” Neuron 51(1):85-97 Details

2003

Greger IH, Khatri L, Kong X, Ziff EB (2003), “AMPA receptor tetramerization is mediated by Q/R editing” Neuron 40:763-774

Greger IH, Khatri L, Kong X, Ziff EB (2003), “AMPA receptor tetramerization is mediated by Q/R editing.” Neuron 40(4):763-74 Details

2002

Greger IH, Khatri L, Ziff EB (2002), “RNA editing at Arg607 controls AMPA receptor exit from the endoplasmic reticulum” Neuron 34:759-772