Professor Eric Barnard
http://www.phar.cam.ac.uk/ri/barn... (personal home page)
We earlier cloned the first GABA-A receptor subunits, the basis of the CNS major inhibitory pathways.We found these vary by their use of different GABA-A subtypes , by combinations drawn from 19 subunits.We have recently analysed their 19 genes in the human genome.
We are studying P2Y receptors for extracellular ATP, based upon our cloning (1993) of the P2Y1 receptor, the first of 9 subtypes.We are identifying cortex and hippocampus neurons on which certain P2Y receptors exist, and .their couplings to specific K and Ca channels ..Recently we showed that ATP is also an inducer of gene transcriptions in neurons, acting via P2Y receptors to regulate synaptic expression of genes for some effectors of ACh and glutamate neurotransmission.
We also find that P2Y receptors form dimers and are characterizing these. We are using modified fluorescence-resonance-energy-transfer confocal analysis to locate and measure such P2Y dimers in living neurons in cultures and in vivo .
Electrophysiological recording techniques
Fluorescence resonance energy transfer
David Brown Web: http://www.ucl.ac.uk/Pharmac...
Richard Evans Web: http://www.le.ac.uk/cpp/
Sasha Filippov Web: http://www.ucl.ac.uk/Pharmac...
Darek Gorecki Web: http://www.port.ac.uk/departm...
Roy Choi Web: http://www.ust.hk/~roychoi
Satya Kunapuli Web: http://spk @temple.edu
Karl Tsim Web: http://www.ust.hk/~botsim
Yung Wong Web: http://www.ust.hk/bich/fa...
Filippov AK, Simon J, Barnard EA, Brown DA (2010), “The scaffold protein NHERF2 determines the coupling of P2Y1 nucleotide and mGluR5 glutamate receptor to different ion channels in neurons.” J Neurosci 30(33):11068-72 Details
Siow NL, Choi RC, Xie HQ, Kong LW, Chu GK, Chan GK, Simon J, Barnard EA, Tsim KW. (2010), “ATP induces synaptic gene expressions in cortical neurons: transduction and transcription control via P2Y1 receptors.” Mol Pharmacol. Sep 16. [ Epub ahead of print ].
Choi RCY,Simon J, Tsim KWK, Barnard EA (2008), “Constitutive and agonist-induced dimerizations of the P2Y1 receptor: relationship to internalization and scaffolding. ” J Biol Chem 283,11050-11063 PDF
Filippov AK, Choi RCY, Simon J, Barnard EA, Brown DA (2006), “Activation of P2Y1 nucleotide receptors induces inhibition of the M-Type K+ current in rat hippocampal pyramidal neurons” J Neurosci 26:9340-9348
Simon J, Wakimoto H, Fujita N, Lalande M, Barnard EA (2004), “Analysis of the full set of GABA-A and Glycine receptor genes in the human genome” J Biol Chem 279:41422-41435 Details
Simon J, Filippov AK, Göransson S, Wong YH, Frelin C, Michel AD, Brown DA, Barnard EA (2002), “Characterization and channel coupling of the P2Y12 nucleotide receptor of brain capillary endothelial cells” J Biol Chem 277:31390-31400