Dr Claire Durrant (née Harwell)


University position

Research Associate

Departments

Department of Clinical Neurosciences

Institutes

Cambridge Centre for Brain Repair

Email

csh45@cam.ac.uk

Home page

https://www.linkedin.com/in/clai... (personal home page)

Research Theme

Cellular and Molecular Neuroscience

Interests

My research aims to model aspects of Alzheimer's disease pathology using organotypic hippocampal slice cultures from APP mutant mice. By maintaining slice cultures from TgCRND8 mice for several months in culture, the progressive accumulation of Aβ and loss of presynaptic proteins can be observed (Harwell & Coleman 2016). As synapse loss is the best correlate of cognitive deficits in Alzheimer's disease, understanding the mechanisms behind this loss may serve to identify novel therapeutic targets. Current work is seeking to identify the causes of presynaptic protein loss in our model as well as attempting to rescue this phenotype using a number of pharmacological and genetic interventions.

Further work seeks to examine other neurodegenerative disease models using the organotypic slice culture technique, with the aim of identifying common targets and mechanisms.

Research Focus

Keywords

Alzheimer's

Organotypic Hippocampal Slice Cultures

Tau

Synapse

Clinical conditions

ALS

Alzheimer's disease

Dementia

Frontotemporal Dementia

Huntington's Disease

Parkinson's Disease

Equipment

Cell culture

Confocal microscopy

ELISA

Fluorescence microscopy

Live Imaging

Organotypic Slice Culture

Western Blot

Collaborators

No collaborators listed

Associated News Items


Key publications

Harwell CS, Coleman MP (2016), “Synaptophysin depletion and intraneuronal Aβ in organotypic hippocampal slice cultures from huAPP transgenic mice” Mol Neurodegener 11:44