Professor Clemens Kaminski

Clemens Kaminski

University position

Professor

Professor Clemens Kaminski is pleased to consider applications from prospective PhD students.

Departments

Department of Chemical Engineering and Biotechnology

Email

cfk23@cam.ac.uk

Home page

http://laser.ceb.cam.ac.uk/

Research Theme

Cellular and Molecular Neuroscience

Interests

We develop advanced microscopic imaging techniques that permit us to elucidate molecular mechanisms of neurodegeneration.

We use techniques such as lifetime, spectrum and polarisation resolved imaging that inform on protein misfolding, aggregation and interaction in live cells and organisms. Recently we have been able to follow the aggregation of Alpha synuclein in a C elegans model of Parkinson's disease and were able to correlate the appearance of aggregates with toxic phenotypes. We have also set up an optical superresolution microscope in Cambridge, based on dSTORM (direct Stochastic Optical Reconstruction Microscopy) and this permits us to visualise morphology and size of toxic oligomers at a resolution down to 10 nm.

Superresolution imaging offers size specific and morphological information on protein amyloids in vitro and in cells and permits us to distinguish between different oligomeric species. The image shows a conventional DIC image shown in grey and the type of aggregates of Aβ that can be seen in the incubating solution exterior to the cells and those found inside the cell which are seen to be structurally different.
Superresolution imaging offers size specific and morphological information on protein amyloids in vitro and in cells and permits us to distinguish between different oligomeric species. The image shows a conventional DIC image shown in grey and the type of aggregates of Aβ that can be seen in the incubating solution exterior to the cells and those found inside the cell which are seen to be structurally different.
Click image to view full-size

Research Focus

Keywords

optical microscopy

protein misfolding and aggregation

live cell imaging

Clinical conditions

Alzheimer's disease

Dementia

Huntington's disease

Parkinson's disease

Equipment

Cell culture

Confocal microscopy

Fluorescence Lifetime Imaging

Fluorescence microscopy

Immunohistochemistry

Intracellular recording

Microscopy

Polarisation resolved imaging

Spectral imaging

Superresolution Microscopy

Collaborators

Cambridge

Chris Dobson

Bill Harris

David Ron

Peter St George-Hyslop

International

Eckhard Mandelkow Web: http://tinyurl.com/3ghecun

Associated News Items


Key publications

Kaminski Schierle GS, Bertoncini CW, Chan FT, van der Goot AT, Schwedler S, Skepper J, Schlachter S, van Ham T, Esposito A, Kumita JR, Nollen EA, Dobson CM, Kaminski CF (2011), “A FRET sensor for non-invasive imaging of amyloid formation in vivo.” Chemphyschem 12(3):673-80 PDF Details

Kaminski Schierle GS, van de Linde S, Erdelyi M, Esbjörner EK, Klein T, Rees E, Bertoncini CW, Dobson CM, Sauer M, Kaminski CF (2011), “In Situ Measurements of the Formation and Morphology of Intracellular β-Amyloid Fibrils by Super-Resolution Fluorescence Imaging.” J Am Chem Soc 133(33):12902-12905 PDF Details

Elder AD, Domin A, Kaminski Schierle GS, Lindon C, Pines J, Esposito A, Kaminski CF (2009), “A quantitative protocol for dynamic measurements of protein interactions by FRET-sensitized fluorescence emission” J. Roy. Soc. Interface 6,59-81 PDF

Publications

2014

Erdelyi M, Simon J, Barnard EA, Kaminski CF (2014), “Analyzing Receptor Assemblies in the Cell Membrane Using Fluorescence Anisotropy Imaging with TIRF Microscopy.” PLoS One 9(6):e100526 Details

Esbjörner EK, Chan F, Rees E, Erdelyi M, Luheshi LM, Bertoncini CW, Kaminski CF, Dobson CM, Kaminski Schierle GS (2014), “Direct Observations of Amyloid β Self-Assembly in Live Cells Provide Insights into Differences in the Kinetics of Aβ(1-40) and Aβ(1-42) Aggregation.” Chem Biol 21(6):732-42 Details

Kaminski CF, Rees EJ, Schierle GS (2014), “A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging.” Methods Mol Biol 1076:445-54 Details

Michel CH, Kumar S, Pinotsi D, Tunnacliffe A, St George-Hyslop P, Mandelkow E, Mandelkow EM, Kaminski CF, Kaminski Schierle GS (2014), “Extracellular monomeric tau protein is sufficient to initiate the spread of tau protein pathology.” J Biol Chem 289(2):956-67 Details

Pinotsi D, Buell AK, Galvagnion C, Dobson CM, Kaminski Schierle GS, Kaminski CF (2014), “Direct observation of heterogeneous amyloid fibril growth kinetics via two-color super-resolution microscopy.” Nano Lett 14(1):339-45 Details

Sinkó J, Kákonyi R, Rees E, Metcalf D, Knight AE, Kaminski CF, Szabó G, Erdélyi M (2014), “TestSTORM: Simulator for optimizing sample labeling and image acquisition in localization based super-resolution microscopy.” Biomed Opt Express 5(3):778-87 Details

2013

Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, Melo EP, Kaminski CF, Ron D (2013), “Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox.” J Cell Biol 201(2):337-49 Details

Chan FT, Kaminski Schierle GS, Kumita JR, Bertoncini CW, Dobson CM, Kaminski CF (2013), “Protein amyloids develop an intrinsic fluorescence signature during aggregation.” Analyst 138(7):2156-62 Details

Erdelyi M, Rees E, Metcalf D, Schierle GS, Dudas L, Sinko J, Knight AE, Kaminski CF (2013), “Correcting chromatic offset in multicolor super-resolution localization microscopy.” Opt Express 21(9):10978-88 Details

Gooding C, Edge C, Lorenz M, Coelho MB, Winters M, Kaminski CF, Cherny D, Eperon IC, Smith CW (2013), “MBNL1 and PTB cooperate to repress splicing of Tpm1 exon 3.” Nucleic Acids Res 41(9):4765-82 Details

Jeyasekharan AD, Liu Y, Hattori H, Pisupati V, Jonsdottir AB, Rajendra E, Lee M, Sundaramoorthy E, Schlachter S, Kaminski CF, Ofir-Rosenfeld Y, Sato K, Savill J, Ayoub N, Venkitaraman AR (2013), “A cancer-associated BRCA2 mutation reveals masked nuclear export signals controlling localization.” Nat Struct Mol Biol 20(10):1191-8 Details

Pinotsi D, Buell AK, Dobson CM, Kaminski Schierle GS, Kaminski CF (2013), “A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence.” Chembiochem 14(7):846-50 Details

Qu Z, Engstrom J, Wong D, Islam M, Kaminski CF (2013), “High sensitivity liquid phase measurements using broadband cavity enhanced absorption spectroscopy (BBCEAS) featuring a low cost webcam based prism spectrometer.” Analyst 138(21):6372-9 Details

2012

Ahn M, De Genst E, Kaminski Schierle GS, Erdelyi M, Kaminski CF, Dobson CM, Kumita JR (2012), “Analysis of the native structure, stability and aggregation of biotinylated human lysozyme.” PLoS One 7(11):e50192 Details

Chakrabortee S, Tripathi R, Watson M, Schierle GS, Kurniawan DP, Kaminski CF, Wise MJ, Tunnacliffe A (2012), “Intrinsically disordered proteins as molecular shields.” Mol Biosyst 8(1):210-9 Details

Kiwanuka SS, Laurila TK, Frank JH, Esposito A, Blomberg von der Geest K, Pancheri L, Stoppa D, Kaminski CF (2012), “Development of broadband cavity ring-down spectroscopy for biomedical diagnostics of liquid analytes.” Anal Chem 84(13):5489-93 Details

Liu C, Rees EJ, Laurila T, Jian S, Kaminski CF (2012), “Periodic interactions between solitons and dispersive waves during the generation of non-coherent supercontinuum radiation.” Opt Express 20(6):6316-24 Details

Murakami T, Yang SP, Xie L, Kawano T, Fu D, Mukai A, Bohm C, Chen F, Robertson J, Suzuki H, Tartaglia GG, Vendruscolo M, Kaminski Schierle GS, Chan FT, Moloney A, Crowther D, Kaminski CF, Zhen M, St George-Hyslop P (2012), “ALS mutations in FUS cause neuronal dysfunction and death in Caenorhabditis elegans by a dominant gain-of-function mechanism.” Hum Mol Genet 21(1):1-9 Details

2011

Chan FT, Kaminski CF, Kaminski Schierle GS (2011), “HomoFRET fluorescence anisotropy imaging as a tool to study molecular self-assembly in live cells.” Chemphyschem 12(3):500-9 PDF Details

Esposito A, Bader AN, Schlachter SC, van den Heuvel DJ, Schierle GS, Venkitaraman AR, Kaminski CF, Gerritsen HC (2011), “Design and application of a confocal microscope for spectrally resolved anisotropy imaging.” Opt Express 19(3):2546-55 PDF Details

Kaminski CF, Sackmann E, Schulten K (2011), “Theodor Förster: a giant of modern photochemistry.” Chemphyschem 12(3):423-4 Details

Laurila T, Sorvajärvi T, Saarela J, Toivonen J, Wheeler DW, Ciaffoni L, Ritchie GA, Kaminski CF (2011), “Optical detection of the anesthetic agent propofol in the gas phase.” Anal Chem 83(10):3963-7 PDF Details

Mauritz JM, Seear R, Esposito A, Kaminski CF, Skepper JN, Warley A, Lew VL, Tiffert T (2011), “X-ray microanalysis investigation of the changes in Na, K, and hemoglobin concentration in plasmodium falciparum-infected red blood cells.” Biophys J 100(6):1438-45 PDF Details

Steuwe C, Kaminski CF, Baumberg JJ, Mahajan S (2011), “Surface enhanced coherent anti-stokes Raman scattering on nanostructured gold surfaces.” Nano Lett 11(12):5339-43 Details

2010

Chakrabortee S, Meersman F, Kaminski Schierle GS, Bertoncini CW, McGee B, Kaminski CF, Tunnacliffe A (2010), “Catalytic and chaperone-like functions in an intrinsically disordered protein associated with desiccation tolerance.” Proc Natl Acad Sci U S A 107(37):16084-9 PDF Details

Cheung W, Gill M, Esposito A, Kaminski CF, Courousse N, Chwetzoff S, Trugnan G, Keshavan N, Lever A, Desselberger U (2010), “Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication.” J Virol 84(13):6782-98 PDF Details

Esposito A, Choimet JB, Skepper JN, Mauritz JM, Lew VL, Kaminski CF, Tiffert T (2010), “Quantitative imaging of human red blood cells infected with Plasmodium falciparum.” Biophys J 99(3):953-60 Details

Kiwanuka SS, Laurila T, Kaminski CF (2010), “Sensitive method for the kinetic measurement of trace species in liquids using cavity enhanced absorption spectroscopy with broad bandwidth supercontinuum radiation.” Anal Chem 82(17):7498-501 PDF Details

Liu C, Rees EJ, Laurila T, Jian S, Kaminski CF (2010), “Predicting supercontinuum pulse collisions with simulations exhibiting temporal aliasing.” Opt Lett 35(24):4145-7 Details

Liu C, Rees EJ, Laurila T, Jian S, Kaminski CF (2010), “An adaptive filter for studying the life cycle of optical rogue waves.” Opt Express 18(25):26113-22 Details

Mauritz JM, Esposito A, Tiffert T, Skepper JN, Warley A, Yoon YZ, Cicuta P, Lew VL, Guck JR, Kaminski CF (2010), “Biophotonic techniques for the study of malaria-infected red blood cells.” Med Biol Eng Comput 48(10):1055-63 PDF Details

Mauritz JM, Morrisby RS, Hutton RS, Legge CH, Kaminski CF (2010), “Imaging pharmaceutical tablets with optical coherence tomography.” J Pharm Sci 99(1):385-91 PDF Details

Mauritz JM, Tiffert T, Seear R, Lautenschläger F, Esposito A, Lew VL, Guck J, Kaminski CF (2010), “Detection of Plasmodium falciparum-infected red blood cells by optical stretching.” J Biomed Opt 15(3):030517 Details

Swietach P, Tiffert T, Mauritz JM, Seear R, Esposito A, Kaminski CF, Lew VL, Vaughan-Jones RD (2010), “Hydrogen ion dynamics in human red blood cells.” J Physiol 588(Pt 24):4995-5014 Details

van Ham TJ, Esposito A, Kumita JR, Hsu ST, Kaminski Schierle GS, Kaminski CF, Dobson CM, Nollen EA, Bertoncini CW (2010), “Towards multiparametric fluorescent imaging of amyloid formation: studies of a YFP model of alpha-synuclein aggregation.” J Mol Biol 395(3):627-42 Details

2009

Abdolvand A, Nazarkin A, Chugreev AV, Kaminski CF, Russell PS (2009), “Solitary pulse generation by backward Raman scattering in H2-filled photonic crystal fibers.” Phys Rev Lett 103(18):183902 Details

Chrystie RS, Burns IS, Hult J, Kaminski CF (2009), “High-repetition-rate combustion thermometry with two-line atomic fluorescence excited by diode lasers.” Opt Lett 34(16):2492-4 Details

Elder AD, Kaminski CF, Frank JH (2009), “phi2FLIM: a technique for alias-free frequency domain fluorescence lifetime imaging.” Opt Express 17(25):23181-203 PDF Details

Esposito A, Schlachter S, Schierle GS, Elder AD, Diaspro A, Wouters FS, Kaminski CF, Iliev AI (2009), “Quantitative fluorescence microscopy techniques.” Methods Mol Biol 586:117-42 Details

Mauritz JM, Esposito A, Ginsburg H, Kaminski CF, Tiffert T, Lew VL (2009), “The homeostasis of Plasmodium falciparum-infected red blood cells.” PLoS Comput Biol 5(4):e1000339 PDF Details

Schlachter S, Elder AD, Esposito A, Kaminski GS, Frank JH, van Geest LK, Kaminski CF (2009), “mhFLIM: resolution of heterogeneous fluorescence decays in widefield lifetime microscopy.” Opt Express 17(3):1557-70 PDF Details

Schlachter S, Schwedler S, Esposito A, Kaminski Schierle GS, Moggridge GD, Kaminski CF (2009), “A method to unmix multiple fluorophores in microscopy images with minimal a priori information.” Opt Express 17(25):22747-60 Details

Watt RS, Laurila T, Kaminski CF, Hult J (2009), “Cavity enhanced spectroscopy of high-temperature H(2)o in the near-infrared using a supercontinuum light source.” Appl Spectrosc 63(12):1389-95 Details

2008

Dai X, Yue Z, Eccleston ME, Swartling J, Slater NK, Kaminski CF (2008), “Fluorescence intensity and lifetime imaging of free and micellar-encapsulated doxorubicin in living cells.” Nanomedicine 4(1):49-56 Details

Dai X, Zhou F, Khan N, Huck WT, Kaminski CF (2008), “Direct visualization of reversible switching of micropatterned polyelectrolyte brushes on gold surfaces using laser scanning confocal microscopy.” Langmuir 24(22):13182-5 PDF Details

Elder A, Schlachter S, Kaminski CF (2008), “Theoretical investigation of the photon efficiency in frequency-domain fluorescence lifetime imaging microscopy.” J Opt Soc Am A Opt Image Sci Vis 25(2):452-62 Details

Esposito A, Tiffert T, Mauritz JM, Schlachter S, Bannister LH, Kaminski CF, Lew VL (2008), “FRET imaging of hemoglobin concentration in Plasmodium falciparum-infected red cells.” PLoS One 3(11):e3780 PDF Details

Euser TG, Whyte G, Scharrer M, Chen JS, Abdolvand A, Nold J, Kaminski CF, Russell PS (2008), “Dynamic control of higher-order modes in hollow-core photonic crystal fibers.” Opt Express 16(22):17972-81 Details

Fidalgo LM, Whyte G, Bratton D, Kaminski CF, Abell C, Huck WT (2008), “From microdroplets to microfluidics: selective emulsion separation in microfluidic devices.” Angew Chem Int Ed Engl 47(11):2042-5 PDF Details

Langridge JM, Laurila T, Watt RS, Jones RL, Kaminski CF, Hult J (2008), “Cavity enhanced absorption spectroscopy of multiple trace gas species using a supercontinuum radiation source.” Opt Express 16(14):10178-88 Details

2007

Frank JH, Elder AD, Swartling J, Venkitaraman AR, Jeyasekharan AD, Kaminski CF (2007), “A white light confocal microscope for spectrally resolved multidimensional imaging.” J Microsc 227(Pt 3):203-15 Details

Hult J, Watt RS, Kaminski CF (2007), “High bandwidth absorption spectroscopy with a dispersed supercontinuum source.” Opt Express 15(18):11385-95 Details

Matthews SM, Elder AD, Yunus K, Kaminski CF, Brennan CM, Fisher AC (2007), “Quantitative kinetic analysis in a microfluidic device using frequency-domain fluorescence lifetime imaging.” Anal Chem 79(11):4101-9 Details

2006

Burns IS, Hult J, Kaminski CF (2006), “Use of (130)Te(2) for frequency referencing and active stabilisation of a violet extended cavity diode laser.” Spectrochim Acta A Mol Biomol Spectrosc 63(5):905-9 Details

Elder AD, Frank JH, Swartling J, Dai X, Kaminski CF (2006), “Calibration of a wide-field frequency-domain fluorescence lifetime microscopy system using light emitting diodes as light sources.” J Microsc 224(Pt 2):166-80 Details

Elder AD, Matthews SM, Swartling J, Yunus K, Frank JH, Brennan CM, Fisher AC, Kaminski CF (2006), “Application of frequency-domain Fluorescence Lifetime Imaging Microscopy as a quantitative analytical tool for microfluidic devices.” Opt Express 14(12):5456-67 Details

2005

Hult J, Burns lS, Kaminski CF (2005), “Wide-bandwidth mode-hop-free tuning of extended-cavity GaN diode lasers.” Appl Opt 44(18):3675-85 Details

2004

Hult J, Burns IS, Kaminski CF (2004), “Measurements of the indium hyperfine structure in an atmospheric-pressure flame by use of diode-laser-induced fluorescence.” Opt Lett 29(8):827-9 Details

2003

Metz T, Walewski J, Kaminski CF (2003), “Maximum-likelihood curve-fitting scheme for experiments with pulsed lasers subject to intensity fluctuations.” Appl Opt 42(9):1551-63 Details

2000

Engström J, Nygren J, Aldén M, Kaminski CF (2000), “Two-line atomic fluorescence as a temperature probe for highly sooting flames.” Opt Lett 25(19):1469-71 Details

Kaminski CF, Dreier T (2000), “Investigation of two-photon-induced polarization spectroscopy of the a-X (1,0) transition in molecular nitrogen at elevated pressures.” Appl Opt 39(6):1042-8 Details

Malm H, Sparr G, Hult J, Kaminski CF (2000), “Nonlinear diffusion filtering of images obtained by planar laser-induced fluorescence spectroscopy.” J Opt Soc Am A Opt Image Sci Vis 17(12):2148-56 Details

1997

Kaminski CF, Ewart P (1997), “Multiplex H2 coherent anti-Stokes Raman scattering thermometry with a modeless laser.” Appl Opt 36(3):731-4 Details