Professor Clemens Kaminski

Clemens Kaminski

University position

Professor

Professor Clemens Kaminski is pleased to consider applications from prospective PhD students.

Departments

Department of Chemical Engineering

Email

cfk23@cam.ac.uk

Home page

http://laser.cheng.cam.ac.uk/

Research Theme

Cellular and Molecular Neuroscience

The Cambridge Neuroscience Community

Interests

We develop advanced microscopic imaging techniques that permit us to elucidate molecular mechanisms of neurodegeneration.

We use techniques such as lifetime, spectrum and polarisation resolved imaging that inform on protein misfolding, aggregation and interaction in live cells and organisms. Recently we have been able to follow the aggregation of Alpha synuclein in a C elegans model of Parkinson's disease and were able to correlate the appearance of aggregates with toxic phenotypes. We have also set up an optical superresolution microscope in Cambridge, based on dSTORM (direct Stochastic Optical Reconstruction Microscopy) and this permits us to visualise morphology and size of toxic oligomers at a resolution down to 10 nm.

Superresolution imaging offers size specific and morphological information on protein amyloids in vitro and in cells and permits us to distinguish between different oligomeric species. The image shows a conventional DIC image shown in grey and the type of aggregates of Aβ that can be seen in the incubating solution exterior to the cells and those found inside the cell which are seen to be structurally different.
Superresolution imaging offers size specific and morphological information on protein amyloids in vitro and in cells and permits us to distinguish between different oligomeric species. The image shows a conventional DIC image shown in grey and the type of aggregates of Aβ that can be seen in the incubating solution exterior to the cells and those found inside the cell which are seen to be structurally different.
Click image to view full-size

Research Focus

Keywords

optical microscopy

protein misfolding and aggregation

live cell imaging

Clinical conditions

Alzheimer's disease

Dementia

Huntington's disease

Parkinson's disease

Equipment

Cell culture

Confocal microscopy

Fluorescence Lifetime Imaging

Fluorescence microscopy

Immunohistochemistry

Intracellular recording

Microscopy

Polarisation resolved imaging

Spectral imaging

Superresolution Microscopy

Collaborators

Cambridge

Richard Adams

Chris Dobson

Bill Harris

Peter St George-Hyslop

International

Eckhard Mandelkow Web: http://tinyurl.com/3ghecun

Key publications

Kaminski Schierle GS, Bertoncini CW, Chan FT, van der Goot AT, Schwedler S, Skepper J, Schlachter S, van Ham T, Esposito A, Kumita JR, Nollen EA, Dobson CM, Kaminski CF (2011), “A FRET sensor for non-invasive imaging of amyloid formation in vivo.” Chemphyschem 12(3):673-80 PDF Details

Kaminski Schierle GS, van de Linde S, Erdelyi M, Esbjörner EK, Klein T, Rees E, Bertoncini CW, Dobson CM, Sauer M, Kaminski CF (2011), “In Situ Measurements of the Formation and Morphology of Intracellular β-Amyloid Fibrils by Super-Resolution Fluorescence Imaging.” J Am Chem Soc 133(33):12902-12905 PDF Details

Elder AD, Domin A, Kaminski Schierle GS, Lindon C, Pines J, Esposito A, Kaminski CF (2009), “A quantitative protocol for dynamic measurements of protein interactions by FRET-sensitized fluorescence emission” J. Roy. Soc. Interface 6,59-81 PDF

Publications

2011

Chan FT, Kaminski CF, Kaminski Schierle GS (2011), “HomoFRET fluorescence anisotropy imaging as a tool to study molecular self-assembly in live cells.” Chemphyschem 12(3):500-9 PDF Details

Esposito A, Bader AN, Schlachter SC, van den Heuvel DJ, Schierle GS, Venkitaraman AR, Kaminski CF, Gerritsen HC (2011), “Design and application of a confocal microscope for spectrally resolved anisotropy imaging.” Opt Express 19(3):2546-55 PDF Details

Laurila T, Sorvajärvi T, Saarela J, Toivonen J, Wheeler DW, Ciaffoni L, Ritchie GA, Kaminski CF (2011), “Optical detection of the anesthetic agent propofol in the gas phase.” Anal Chem 83(10):3963-7 PDF Details

Mauritz JM, Seear R, Esposito A, Kaminski CF, Skepper JN, Warley A, Lew VL, Tiffert T (2011), “X-ray microanalysis investigation of the changes in Na, K, and hemoglobin concentration in plasmodium falciparum-infected red blood cells.” Biophys J 100(6):1438-45 PDF Details

2010

Chakrabortee S, Meersman F, Kaminski Schierle GS, Bertoncini CW, McGee B, Kaminski CF, Tunnacliffe A (2010), “Catalytic and chaperone-like functions in an intrinsically disordered protein associated with desiccation tolerance.” Proc Natl Acad Sci U S A 107(37):16084-9 PDF Details

Cheung W, Gill M, Esposito A, Kaminski CF, Courousse N, Chwetzoff S, Trugnan G, Keshavan N, Lever A, Desselberger U (2010), “Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication.” J Virol 84(13):6782-98 PDF Details

Esposito A, Choimet JB, Skepper JN, Mauritz JM, Lew VL, Kaminski CF, Tiffert T (2010), “Quantitative imaging of human red blood cells infected with Plasmodium falciparum.” Biophys J 99(3):953-60 Details

Kiwanuka SS, Laurila T, Kaminski CF (2010), “Sensitive method for the kinetic measurement of trace species in liquids using cavity enhanced absorption spectroscopy with broad bandwidth supercontinuum radiation.” Anal Chem 82(17):7498-501 PDF Details

Mauritz JM, Esposito A, Tiffert T, Skepper JN, Warley A, Yoon YZ, Cicuta P, Lew VL, Guck JR, Kaminski CF (2010), “Biophotonic techniques for the study of malaria-infected red blood cells.” Med Biol Eng Comput 48(10):1055-63 PDF Details

Mauritz JM, Morrisby RS, Hutton RS, Legge CH, Kaminski CF (2010), “Imaging pharmaceutical tablets with optical coherence tomography.” J Pharm Sci 99(1):385-91 PDF Details

2009

Elder AD, Kaminski CF, Frank JH (2009), “phi2FLIM: a technique for alias-free frequency domain fluorescence lifetime imaging.” Opt Express 17(25):23181-203 PDF Details

Esposito A, Schlachter S, Schierle GS, Elder AD, Diaspro A, Wouters FS, Kaminski CF, Iliev AI (2009), “Quantitative fluorescence microscopy techniques.” Methods Mol Biol 586:117-42 Details

Mauritz JM, Esposito A, Ginsburg H, Kaminski CF, Tiffert T, Lew VL (2009), “The homeostasis of Plasmodium falciparum-infected red blood cells.” PLoS Comput Biol 5(4):e1000339 PDF Details

Schlachter S, Elder AD, Esposito A, Kaminski GS, Frank JH, van Geest LK, Kaminski CF (2009), “mhFLIM: resolution of heterogeneous fluorescence decays in widefield lifetime microscopy.” Opt Express 17(3):1557-70 PDF Details

2008

Dai X, Yue Z, Eccleston ME, Swartling J, Slater NK, Kaminski CF (2008), “Fluorescence intensity and lifetime imaging of free and micellar-encapsulated doxorubicin in living cells.” Nanomedicine 4(1):49-56 Details

Dai X, Zhou F, Khan N, Huck WT, Kaminski CF (2008), “Direct visualization of reversible switching of micropatterned polyelectrolyte brushes on gold surfaces using laser scanning confocal microscopy.” Langmuir 24(22):13182-5 PDF Details

Elder A, Schlachter S, Kaminski CF (2008), “Theoretical investigation of the photon efficiency in frequency-domain fluorescence lifetime imaging microscopy.” J Opt Soc Am A Opt Image Sci Vis 25(2):452-62 Details

Esposito A, Tiffert T, Mauritz JM, Schlachter S, Bannister LH, Kaminski CF, Lew VL (2008), “FRET imaging of hemoglobin concentration in Plasmodium falciparum-infected red cells.” PLoS One 3(11):e3780 PDF Details

Fidalgo LM, Whyte G, Bratton D, Kaminski CF, Abell C, Huck WT (2008), “From microdroplets to microfluidics: selective emulsion separation in microfluidic devices.” Angew Chem Int Ed Engl 47(11):2042-5 PDF Details

Langridge JM, Laurila T, Watt RS, Jones RL, Kaminski CF, Hult J (2008), “Cavity enhanced absorption spectroscopy of multiple trace gas species using a supercontinuum radiation source.” Opt Express 16(14):10178-88 Details