Dr Tony Jackson
University position
Senior Lecturer
Dr Tony Jackson is pleased to consider applications from prospective PhD students.
Departments
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Research Theme
Interests
1) Sodium channels contain both alpha and beta subunits. The beta subunits regulate channel kinetics and assembly. The beta subunit extracellular region is homologous to the immunoglobulin domain found in cell adhesion molecules, and beta subunits can act as cell adhesion molecules independently of their effects on sodium channels. We identified a new auxiliary subunit, beta 3. We are currently examining the distinct roles of the intracellular and extracellular domains using electrophysiological and cell biological assays of distinct beta 3 mutants.
2) Neuroendocrine cells contain specialized organelles - densecore granules and synaptic vesicles. We have identified a variant of the PC12 neuroendocrine cell line that selectively lacks these organelles. We have used microarray profiling to catalogue the protein components selectively down regulated in the variant and have identified a transcriptional repressor that plays a role in maintaining the neurosecretory-deficient phenotype.
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Research Focus
Keywordssodium channels membrane traffic cell biology regulated secretion |
Clinical conditionsAlzheimer's disease Epilepsy Genetic disorders |
Equipment
Cell culture
Confocal microscopy
Electrophysiological recording techniques
Enzyme assays
Fluorescence microscopy
Microscopy
Protein purification
Recombinant protein expression
Whole cell patch clamp
Collaborators
CambridgeAndrew Grace Chris Huang | United KingdomJeff Clare Web: http://www.gsk.com/index.htm InternationalManoj Patel Web: http://www.healthsystem.virginia.edu/interne... Revaz Solomonia Web: http://www.acnet.ge/ |
Key publications
Pance A, Livesey FJ, Jackson AP (2006), “A role for the transcriptional repressor REST in maintaining the phenotype of neurosecretory-deficient PC12 cells” J Neurochem 99(5):1435-44 Details
Yu EJ, Ko SH, Lenkowski PW, Pance A, Patel MK, Jackson AP (2005), “Distinct domains of the sodium channel beta3-subunit modulate channel-gating kinetics and subcellular location” Biochem J 392(Pt 3):519-26 Details
Morgan K, Stevens EB, Shah B, Cox PJ, Dixon AK, Lee K, Pinnock RD, Hughes J, Richardson PJ, Mizuguchi K, Jackson AP (2000), “beta 3: an additional auxiliary subunit of the voltage-sensitive sodium channel that modulates channel gating with distinct kinetics” Proc Natl Acad Sci U S A 97(5):2308-13
Publications
2010
Cusdin FS, Nietlispach D, Maman J, Dale TJ, Powell AJ, Clare JJ, Jackson AP (2010), “The sodium channel {beta}3-subunit induces multiphasic gating in Nav1.3 and affects fast inactivation via distinct intracellular regions.” J Biol Chem Details
Hakim P, Brice N, Thresher R, Lawrence J, Zhang Y, Jackson AP, Grace AA, Huang CL (2010), “Scn3b knockout mice exhibit abnormal sino-atrial and cardiac conduction properties.” Acta Physiol (Oxf) 198(1):47-59 Details
Merrick EC, Kalmar CL, Snyder SL, Cusdin FS, Yu EJ, Sando JJ, Isakson BE, Jackson AP, Patel MK (2010), “The importance of serine 161 in the sodium channel beta3 subunit for modulation of Na(V)1.2 gating.” Pflugers Arch 460(4):743-53 Details
Neumann-Staubitz P, Hall SL, Kuo J, Jackson AP (2010), “Characterization of a temperature-sensitive vertebrate clathrin heavy chain mutant as a tool to study clathrin-dependent events in vivo.” PLoS One 5(8) Details
Zhang C, Jackson AP, Zhang ZR, Han Y, Yu S, He RQ, Perrett S (2010), “Amyloid-like aggregates of the yeast prion protein ure2 enter vertebrate cells by specific endocytotic pathways and induce apoptosis.” PLoS One 5(9) Details
2009
Hall SL, Hester S, Griffin JL, Lilley KS, Jackson AP (2009), “The organelle proteome of the DT40 lymphocyte cell line.” Mol Cell Proteomics 8(6):1295-305 Details
van Gassen KL, de Wit M, van Kempen M, van der Hel WS, van Rijen PC, Jackson AP, Lindhout D, de Graan PN (2009), “Hippocampal Nabeta3 expression in patients with temporal lobe epilepsy.” Epilepsia 50(4):957-62 Details
2008
Borlido J, Veltri G, Jackson AP, Mills IG (2008), “Clathrin is spindle-associated but not essential for mitosis.” PLoS One 3(9):e3115 Details
Cusdin FS, Clare JJ, Jackson AP (2008), “Trafficking and cellular distribution of voltage-gated sodium channels.” Traffic 9(1):17-26 Details
Solomonia RO, Apkhazava D, Nozadze M, Jackson AP, McCabe BJ, Horn G (2008), “Different forms of MARCKS protein are involved in memory formation in the learning process of imprinting.” Exp Brain Res 188(2):323-30 Details
2006
Courel M, Rodemer C, Nguyen ST, Pance A, Jackson AP, O'connor DT, Taupenot L (2006), “Secretory granule biogenesis in sympathoadrenal cells: identification of a granulogenic determinant in the secretory prohormone chromogranin A.” J Biol Chem 281(49):38038-51 Details
Wettey FR, Jackson AP (2006), “Quantification of receptor-mediated endocytosis.” Subcell Biochem 40:431-4 Details
Wettey FR, Jackson AP (2006), “Indirect immunofluorescence microscopy.” Subcell Biochem 40:427-9 Details
Wettey FR, Jackson AP (2006), “Luciferase reporter assay.” Subcell Biochem 40:423-5 Details
Wettey FR, Jackson AP (2006), “Using DT40 to study clathrin function.” Subcell Biochem 40:119-43 Details
2005
Bentrari F, Arnould L, Jackson AP, Jeannin JF, Pance A (2005), “Progesterone enhances cytokine-stimulated nitric oxide synthase II expression and cell death in human breast cancer cells.” Lab Invest 85(5):624-32 Details
Diaz-Griffero F, Jackson AP, Brojatsch J (2005), “Cellular uptake of avian leukosis virus subgroup B is mediated by clathrin.” Virology 337(1):45-54 Details
Flett A, Semerdjieva S, Jackson AP, Smythe E (2005), “Regulation of the clathrin-coated vesicle cycle by reversible phosphorylation.” Biochem Soc Symp (72):65-70 Details
Stoddart A, Jackson AP, Brodsky FM (2005), “Plasticity of B cell receptor internalization upon conditional depletion of clathrin.” Mol Biol Cell 16(5):2339-48 Details
2003
Jackson AP, Flett A, Smythe C, Hufton L, Wettey FR, Smythe E (2003), “Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor micro2 kinase.” J Cell Biol 163(2):231-6 Details
Solomonia RO, Morgan K, Kotorashvili A, McCabe BJ, Jackson AP, Horn G (2003), “Analysis of differential gene expression supports a role for amyloid precursor protein and a protein kinase C substrate (MARCKS) in long-term memory.” Eur J Neurosci 17(5):1073-81 Details
2002
Wettey FR, Hawkins SF, Stewart A, Luzio JP, Howard JC, Jackson AP (2002), “Controlled elimination of clathrin heavy-chain expression in DT40 lymphocytes.” Science 297(5586):1521-5 Details
2001
Fahmi AI, Patel M, Stevens EB, Fowden AL, John JE 3rd, Lee K, Pinnock R, Morgan K, Jackson AP, Vandenberg JI (2001), “The sodium channel beta-subunit SCN3b modulates the kinetics of SCN5a and is expressed heterogeneously in sheep heart.” J Physiol 537(Pt 3):693-700 Details
Razzaq A, Robinson IM, McMahon HT, Skepper JN, Su Y, Zelhof AC, Jackson AP, Gay NJ, O'Kane CJ (2001), “Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in Drosophila.” Genes Dev 15(22):2967-79 Details
2000
Morgan K, Stevens EB, Shah B, Cox PJ, Dixon AK, Lee K, Pinnock RD, Hughes J, Richardson PJ, Mizuguchi K, Jackson AP (2000), “beta 3: an additional auxiliary subunit of the voltage-sensitive sodium channel that modulates channel gating with distinct kinetics.” Proc Natl Acad Sci U S A 97(5):2308-13 Details
Razzaq A, Su Y, Mehren JE, Mizuguchi K, Jackson AP, Gay NJ, O'Kane CJ (2000), “Characterisation of the gene for Drosophila amphiphysin.” Gene 241(1):167-74 Details
1999
Pance A, Morgan K, Guest PC, Bowers K, Dean GE, Cutler DF, Jackson AP (1999), “A PC12 variant lacking regulated secretory organelles: aberrant protein targeting and evidence for a factor inhibiting neuroendocrine gene expression.” J Neurochem 73(1):21-30 Details
1998
Billington D, Maltby PJ, Jackson AP, Graham JM (1998), “Dissection of hepatic receptor-mediated endocytic pathways using self-generated gradients of iodixanol (Optiprep).” Anal Biochem 258(2):251-8 Details
1997
Dornan S, Jackson AP, Gay NJ (1997), “Alpha-adaptin, a marker for endocytosis, is expressed in complex patterns during Drosophila development.” Mol Biol Cell 8(8):1391-403 Details
Solomonia RO, McCabe BJ, Jackson AP, Horn G (1997), “Clathrin proteins and recognition memory.” Neuroscience 80(1):59-67 Details
1996
Cheetham ME, Anderton BH, Jackson AP (1996), “Inhibition of hsc70-catalysed clathrin uncoating by HSJ1 proteins.” Biochem J 319 ( Pt 1):103-8 Details
Jackson AP (1996), “The conformation of clathrin light-chains on triskelions probed by antibody inhibition.” Cell Biol Int 20(5):385-90 Details
1994
Cheetham ME, Jackson AP, Anderton BH (1994), “Regulation of 70-kDa heat-shock-protein ATPase activity and substrate binding by human DnaJ-like proteins, HSJ1a and HSJ1b.” Eur J Biochem 226(1):99-107 Details
Ponnambalam S, Jackson AP, LeBeau MM, Pravtcheva D, Ruddle FH, Alibert C, Parham P (1994), “Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB).” Genomics 24(3):440-4 Details
1993
Acton SL, Wong DH, Parham P, Brodsky FM, Jackson AP (1993), “Alteration of clathrin light chain expression by transfection and gene disruption.” Mol Biol Cell 4(6):647-60 Details
Jackson AP (1993), “The isolation of clathrin-coated vesicles and purification of their protein components.” Methods Mol Biol 19:83-96 Details
1992
Jackson AP (1992), “Endocytosis in the brain: the role of clathrin light-chains.” Biochem Soc Trans 20(3):653-5 Details
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