Professor Andrea Brand FRS FMedSci

Andrea Brand

University position

Herchel Smith Professor of Molecular Biology

Professor Andrea Brand FRS FMedSci is pleased to consider applications from prospective PhD students.

Departments

Department of Physiology, Development and Neuroscience

Institutes

Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology

Email

a.brand@gurdon.cam.ac.uk

Home page

http://www.gurdon.cam.ac.uk/researc...

Research Themes

Developmental Neuroscience

Cellular and Molecular Neuroscience

Interests

Discovering how stem cells are maintained in a multipotent state and how their progeny differentiate into distinct cellular fates is a key step in the therapeutic use of stem cells to repair tissues after damage or disease. We are investigating the genetic networks that regulate stem cells in the Drosophila central nervous system. We use classical and reverse genetics, high resolution imaging and genome-wide transcriptional profiling to identify the molecular switches controlling the transition from symmetric to asymmetric division and from self-renewal to differentiation. We are characterising the genetic networks that control the transition from a neural stem cell to a specialised neuronal or glial cell type. By identifying neural stem cell-specific genes, and genes specific for differentiating daughters, we can begin to assess the potential for inducing neurons to divide in a regenerative manner, or for directing stem cells to differentiate into specific neuronal subtypes.

Neural stem cells in the larval brain lobe (Discs large, green; DAPI, blue; histone-RFP, red)
Neural stem cells in the larval brain lobe (Discs large, green; DAPI, blue; histone-RFP, red)
Click image to view full-size

Research Focus

Keywords

stem cells

asymmetric cell division

self-renewal

differentiation

neural circuit

Clinical conditions

Cancers

Equipment

Confocal microscopy

Fluorescence microscopy

Immunohistochemistry

Live imaging

Microscopy

Protein purification

Recombinant protein expression

Single cell analysis

Targeted gene expression

Transcriptional analysis

Collaborators

United Kingdom

Richard Baines Web: http://www.ls.manchester.ac.uk/people/...

Stephen Goodwin Web: http://www.dpag.ox.ac.uk/team/st...

International

Allison Bardin Web: http://ugbdd.curie.fr/en/arti...

Bruce Edgar Web: http://www.zmbh.uni-heidelberg.de/edgar/

Leanne Jones Web: http://joneslabucla.com/

Associated News Items


Key publications

Southall TD, Davidson CM, Miller C, Carr A, Brand AH (2014), “Dedifferentiation of Neurons Precedes Tumor Formation in lola Mutants.” Dev Cell 28(6):685-96 Details

Southall TD, Gold KS, Egger B, Davidson CM, Caygill EE, Marshall OJ, Brand AH (2013), “Cell-type-specific profiling of gene expression and chromatin binding without cell isolation: assaying RNA Pol II occupancy in neural stem cells.” Dev Cell 26(1):101-12 Details

Chell JM, Brand AH (2010), “Nutrition-responsive glia control exit of neural stem cells from quiescence.” Cell 143(7):1161-73 Details

Egger B, Gold KS, Brand AH (2010), “Notch regulates the switch from symmetric to asymmetric neural stem cell division in the Drosophila optic lobe.” Development 137(18):2981-7 Details

Southall TD, Brand AH (2009), “Neural stem cell transcriptional networks highlight genes essential for nervous system development.” EMBO J 28(24):3799-807 Details

Egger B, Boone JQ, Stevens NR, Brand AH, Doe CQ (2007), “Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe” Neural Development 2:1 Details

Choksi SP, Southall T, Bossing T, Edoff K, de Wit E, van Steensel B, Micklem G, Brand AH (2006), “Prospero acts as a binary switch between self-renewal and differentiation in Drosophila neural stem cells” Developmental Cell 11:775-789 Details

van Roessel PJ, Elliott DA, Robinson IM, Prokop A, Brand AH (2004), “Independent regulation of synaptic size and activity by the anaphase-promoting complex” Cell 119:707-718

Publications

2014

Neville MC, Nojima T, Ashley E, Parker DJ, Walker J, Southall T, Van de Sande B, Marques AC, Fischer B, Brand AH, Russell S, Ritchie MG, Aerts S, Goodwin SF (2014), “Male-specific fruitless isoforms target neurodevelopmental genes to specify a sexually dimorphic nervous system.” Curr Biol 24(3):229-41 Details

Wolfram V, Southall TD, Günay C, Prinz AA, Brand AH, Baines RA (2014), “The transcription factors islet and Lim3 combinatorially regulate ion channel gene expression.” J Neurosci 34(7):2538-43 Details

2013

Cheetham SW, Brand AH (2013), “Cell biology. Insulin finds its niche.” Science 340(6134):817-8 Details

2012

Gold KS, Brand AH (2012), “Transcriptome analysis of Drosophila neural stem cells.” Methods Mol Biol 916:99-110 Details

Murray MJ, Southall TD, Liu W, Fraval H, Lorensuhewa N, Brand AH, Saint R (2012), “Snail-dependent repression of the RhoGEF pebble is required for gastrulation consistency in Drosophila melanogaster.” Dev Genes Evol 222(6):361-8 Details

Wolfram V, Southall TD, Brand AH, Baines RA (2012), “The LIM-homeodomain protein islet dictates motor neuron electrical properties by regulating K(+) channel expression.” Neuron 75(4):663-74 Details

2011

Brand AH, Livesey FJ (2011), “Neural stem cell biology in vertebrates and invertebrates: more alike than different?” Neuron 70(4):719-29 Details

Egger B, Gold KS, Brand AH (2011), “Regulating the balance between symmetric and asymmetric stem cell division in the developing brain.” Fly (Austin) 5(3):237-41 Details

Franco M, Seyfried NT, Brand AH, Peng J, Mayor U (2011), “A novel strategy to isolate ubiquitin conjugates reveals wide role for ubiquitination during neural development.” Mol Cell Proteomics 10(5):M110.002188 Details

Spéder P, Liu J, Brand AH (2011), “Nutrient control of neural stem cells.” Curr Opin Cell Biol 23(6):724-9 Details

2010

Bardin AJ, Perdigoto CN, Southall TD, Brand AH, Schweisguth F (2010), “Transcriptional control of stem cell maintenance in the Drosophila intestine.” Development 137(5):705-14 Details

Monier B, Pélissier-Monier A, Brand AH, Sanson B (2010), “An actomyosin-based barrier inhibits cell mixing at compartmental boundaries in Drosophila embryos.” Nat Cell Biol 12(1):60-5; sup pp 1-9 Details

2009

Hensch TK, Brand AH (2009), “Development. Editorial overview.” Curr Opin Neurobiol 19(2):109-11 Details

Ruiz i Altaba A, Brand AH (2009), “Entity versus property: tracking the nature, genesis and role of stem cells in cancer. Conference on Stem cells and cancer.” EMBO Rep 10(8):832-6 Details

von Trotha JW, Egger B, Brand AH (2009), “Cell proliferation in the Drosophila adult brain revealed by clonal analysis and bromodeoxyuridine labelling.” Neural Dev 4:9 Details

2008

Brand AH (2008), “A new dawn for Aurora?” Nat Cell Biol 10(11):1253-4 Details

Chell JM, Brand AH (2008), “Forever young: death-defying neuroblasts.” Cell 133(5):769-71 Details

Egger B, Chell JM, Brand AH (2008), “Insights into neural stem cell biology from flies.” Philos Trans R Soc Lond B Biol Sci 363(1489):39-56 Details

Elliott DA, Brand AH (2008), “The GAL4 system : a versatile system for the expression of genes.” Methods Mol Biol 420:79-95 Details

Southall TD, Brand AH (2008), “Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila.” CSH Protoc 2008:pdb.prot5029 Details

Southall TD, Egger B, Gold KS, Brand AH (2008), “Regulation of self-renewal and differentiation in the Drosophila nervous system.” Cold Spring Harb Symp Quant Biol 73:523-8 Details

Southall TD, Elliott DA, Brand AH (2008), “The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila.” CSH Protoc 2008:pdb.top49 Details

Wu PS, Egger B, Brand AH (2008), “Asymmetric stem cell division: lessons from Drosophila.” Semin Cell Dev Biol 19(3):283-93 Details

2007

Edoff K, Dods JS, Brand AH (2007), “Detection of GFP during nervous system development in Drosophila melanogaster.” Methods Mol Biol 411:81-98 Details

Southall TD, Brand AH (2007), “Chromatin profiling in model organisms.” Brief Funct Genomic Proteomic 6(2):133-40 Details

2006

Barbee SA, Estes PS, Cziko AM, Hillebrand J, Luedeman RA, Coller JM, Johnson N, Howlett IC, Geng C, Ueda R, Brand AH, Newbury SF, Wilhelm JE, Levine RB, Nakamura A, Parker R, Ramaswami M (2006), “Staufen- and FMRP-containing neuronal RNPs are structurally and functionally related to somatic P bodies.” Neuron 52(6):997-1009 Details

Bossing T, Brand AH (2006), “Determination of cell fate along the anteroposterior axis of the Drosophila ventral midline.” Development 133(6):1001-12 Details

Murray MJ, Davidson CM, Hayward NM, Brand AH (2006), “The Fes/Fer non-receptor tyrosine kinase cooperates with Src42A to regulate dorsal closure in Drosophila.” Development 133(16):3063-73 Details

Pym EC, Southall TD, Mee CJ, Brand AH, Baines RA (2006), “The homeobox transcription factor Even-skipped regulates acquisition of electrical properties in Drosophila neurons.” Neural Dev 1:3 Details

2005

Dawes-Hoang RE, Parmar KM, Christiansen AE, Phelps CB, Brand AH, Wieschaus EF (2005), “folded gastrulation, cell shape change and the control of myosin localization.” Development 132(18):4165-78 Details

2004

Carr AR, Choksi SP, Brand AH (2004), “Turning back the clock on neural progenitors.” Bioessays 26(7):711-4 Details

van Roessel P, Brand AH (2004), “Spreading silence with Sid.” Genome Biol 5(2):208 Details

van Roessel P, Elliott DA, Robinson IM, Prokop A, Brand AH (2004), “Independent regulation of synaptic size and activity by the anaphase-promoting complex.” Cell 119(5):707-18 Details

2003

Barros CS, Phelps CB, Brand AH (2003), “Drosophila nonmuscle myosin II promotes the asymmetric segregation of cell fate determinants by cortical exclusion rather than active transport.” Dev Cell 5(6):829-40 Details

Brand AH, van Roessel PJ (2003), “Region-specific apoptosis limits neural stem cell proliferation.” Neuron 37(2):185-7 Details

2002

Bossing T, Barros CS, Brand AH (2002), “Rapid tissue-specific expression assay in living embryos.” Genesis 34(1-2):123-6 Details

Bossing T, Brand AH (2002), “Dephrin, a transmembrane ephrin with a unique structure, prevents interneuronal axons from exiting the Drosophila embryonic CNS.” Development 129(18):4205-18 Details

Januschke J, Gervais L, Dass S, Kaltschmidt JA, Lopez-Schier H, St Johnston D, Brand AH, Roth S, Guichet A (2002), “Polar transport in the Drosophila oocyte requires Dynein and Kinesin I cooperation.” Curr Biol 12(23):1971-81 Details

Kaltschmidt JA, Brand AH (2002), “Asymmetric cell division: microtubule dynamics and spindle asymmetry.” J Cell Sci 115(Pt 11):2257-64 Details

van Roessel P, Brand AH (2002), “Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins.” Nat Cell Biol 4(1):E15-20 Details

Van Roessel P, Hayward NM, Barros CS, Brand AH (2002), “Two-color GFP imaging demonstrates cell-autonomy of GAL4-driven RNA interference in Drosophila.” Genesis 34(1-2):170-3 Details

2001

Bellaïche Y, Gho M, Kaltschmidt JA, Brand AH, Schweisguth F (2001), “Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division.” Nat Cell Biol 3(1):50-7 Details

2000

Kaltschmidt JA, Davidson CM, Brown NH, Brand AH (2000), “Rotation and asymmetry of the mitotic spindle direct asymmetric cell division in the developing central nervous system.” Nat Cell Biol 2(1):7-12 Details

1999

Haseloff J, Dormand EL, Brand AH (1999), “Live imaging with green fluorescent protein.” Methods Mol Biol 122:241-59 Details

Schuldt AJ, Brand AH (1999), “Mastermind acts downstream of notch to specify neuronal cell fates in the Drosophila central nervous system.” Dev Biol 205(2):287-95 Details

1998

Dormand EL, Brand AH (1998), “Runt determines cell fates in the Drosophila embryonic CNS.” Development 125(9):1659-67 Details

Murray MJ, Merritt DJ, Brand AH, Whitington PM (1998), “In vivo dynamics of axon pathfinding in the Drosophilia CNS: a time-lapse study of an identified motorneuron.” J Neurobiol 37(4):607-21 Details

Phelps CB, Brand AH (1998), “Ectopic gene expression in Drosophila using GAL4 system.” Methods 14(4):367-79 Details

Schuldt AJ, Adams JH, Davidson CM, Micklem DR, Haseloff J, St Johnston D, Brand AH (1998), “Miranda mediates asymmetric protein and RNA localization in the developing nervous system.” Genes Dev 12(12):1847-57 Details

1997

Hidalgo A, Brand AH (1997), “Targeted neuronal ablation: the role of pioneer neurons in guidance and fasciculation in the CNS of Drosophila.” Development 124(17):3253-62 Details

1995

Brand AH, Dormand EL (1995), “The GAL4 system as a tool for unravelling the mysteries of the Drosophila nervous system.” Curr Opin Neurobiol 5(5):572-8 Details

Hidalgo A, Urban J, Brand AH (1995), “Targeted ablation of glia disrupts axon tract formation in the Drosophila CNS.” Development 121(11):3703-12 Details

Yoffe KB, Manoukian AS, Wilder EL, Brand AH, Perrimon N (1995), “Evidence for engrailed-independent wingless autoregulation in Drosophila.” Dev Biol 170(2):636-50 Details

1994

Brand AH, Manoukian AS, Perrimon N (1994), “Ectopic expression in Drosophila.” Methods Cell Biol 44:635-54 Details

Brand AH, Perrimon N (1994), “Raf acts downstream of the EGF receptor to determine dorsoventral polarity during Drosophila oogenesis.” Genes Dev 8(5):629-39 Details

Staehling-Hampton K, Jackson PD, Clark MJ, Brand AH, Hoffmann FM (1994), “Specificity of bone morphogenetic protein-related factors: cell fate and gene expression changes in Drosophila embryos induced by decapentaplegic but not 60A.” Cell Growth Differ 5(6):585-93 Details

1993

Brand AH, Perrimon N (1993), “Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.” Development 118(2):401-15 Details

1989

Hofmann JF, Laroche T, Brand AH, Gasser SM (1989), “RAP-1 factor is necessary for DNA loop formation in vitro at the silent mating type locus HML.” Cell 57(5):725-37 Details

1987

Brand AH, Micklem G, Nasmyth K (1987), “A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activation.” Cell 51(5):709-19 Details

Shore D, Stillman DJ, Brand AH, Nasmyth KA (1987), “Identification of silencer binding proteins from yeast: possible roles in SIR control and DNA replication.” EMBO J 6(2):461-7 Details

1985

Brand AH, Breeden L, Abraham J, Sternglanz R, Nasmyth K (1985), “Characterization of a "silencer" in yeast: a DNA sequence with properties opposite to those of a transcriptional enhancer.” Cell 41(1):41-8 Details