Dr Alessandro Esposito

University position

Senior Investigator Scientist

Dr Alessandro Esposito is pleased to consider applications from prospective PhD students.

Departments

MRC Cancer Unit

Institutes

Hutchison/Medical Research Council Research Centre

Email

ae275@mrc-cu.cam.ac.uk

Home page

http://home.quantitative-microscopy.org (personal home page)

Research Theme

Cellular and Molecular Neuroscience

Interests

My goal is to understand how cellular biochemistry encodes decision-making processes, particularly those processes that lead to cancer when misregulated. However, the lack of technologies that permit mapping a multitude of biochemical reactions in the living cell and their correlation with cellular behaviour limits our understanding of these spatio-temporally organized networks. I believe that a new approach to imaging techniques is necessary to overcome these limitations. Currently, I am developping novel technologies, methods and biosensors tailored to multiplex the main nodes of signalling or metabolic networks, permitting, at the same time, acute perturbation of biochemical reactions. The convergence between biochemical imaging and optogenetics is likely to impact how we do and how we understand biology.

Although I used to work on neurodegenerative diseases, now I work on cancer research; my interest in the neurosciences continues thanks to collaborative projects

Research Focus

Keywords

neurodegeneration

microscopy

synuclein

cancer

biosensor

Clinical conditions

Cancers

Equipment

Confocal microscopy

Fluorescence microscopy

Microscopy

Collaborators

Cambridge

Carlos Bertoncini

Gabriele Kaminski Schierle

United Kingdom

Quentin Hanley Web: http://www.ntu.ac.uk/researc...

International

Hans Gerritsen Web: http://www1.phys.uu.nl/wwwmbf/...

Thomas Jovin Web: http://www.mpibpc.mpg.de/groups/...

Fred Wouters Web: http://www.eni-net.org/index.p...

Key publications

Liang H, Esposito A, De S, Ber S, Collin P, Surana U, Venkitaraman AR (2014), “Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing.” Nat Commun 5:4048 Details

Esposito A, Popleteeva M, Venkitaraman AR (2013), “Maximizing the biochemical resolving power of fluorescence microscopy.” PLoS One 8(10):e77392 Details

Esposito A, Bader AN, Schlachter SC, van den Heuvel DJ, Schierle GS, Venkitaraman AR, Kaminski CF, Gerritsen HC (2011), “Design and application of a confocal microscope for spectrally resolved anisotropy imaging.” Opt Express 19(3):2546-55 Details

Kaminski Schierle GS, Bertoncini CW, Chan FT, van der Goot AT, Schwedler S, Skepper J, Schlachter S, van Ham T, Esposito A, Kumita JR, Nollen EA, Dobson CM, Kaminski CF (2011), “A FRET sensor for non-invasive imaging of amyloid formation in vivo.” Chemphyschem 12(3):673-80 Details

van Ham TJ, Esposito A, Kumita JR, Hsu ST, Kaminski Schierle GS, Kaminski CF, Dobson CM, Nollen EA, Bertoncini CW (2010), “Towards multiparametric fluorescent imaging of amyloid formation: studies of a YFP model of alpha-synuclein aggregation.” J Mol Biol 395(3):627-42 Details

Esposito A, Dohm CP, Bähr M, Wouters FS (2007), “Unsupervised fluorescence lifetime imaging microscopy for high content and high throughput screening.” Mol Cell Proteomics 6(8):1446-54 Details

Esposito A, Dohm CP, Kermer P, Bähr M, Wouters FS (2007), “alpha-Synuclein and its disease-related mutants interact differentially with the microtubule protein tau and associate with the actin cytoskeleton.” Neurobiol Dis 26(3):521-31 Details

Publications

2012

Kiwanuka SS, Laurila TK, Frank JH, Esposito A, Blomberg von der Geest K, Pancheri L, Stoppa D, Kaminski CF (2012), “Development of broadband cavity ring-down spectroscopy for biomedical diagnostics of liquid analytes.” Anal Chem 84(13):5489-93 Details

2011

Fereidouni F, Esposito A, Blab GA, Gerritsen HC (2011), “A modified phasor approach for analyzing time-gated fluorescence lifetime images.” J Microsc 244(3):248-58 Details

Mauritz JM, Seear R, Esposito A, Kaminski CF, Skepper JN, Warley A, Lew VL, Tiffert T (2011), “X-ray microanalysis investigation of the changes in Na, K, and hemoglobin concentration in plasmodium falciparum-infected red blood cells.” Biophys J 100(6):1438-45 Details

2010

Cheung W, Gill M, Esposito A, Kaminski CF, Courousse N, Chwetzoff S, Trugnan G, Keshavan N, Lever A, Desselberger U (2010), “Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication.” J Virol 84(13):6782-98 Details

Cueff A, Seear R, Dyrda A, Bouyer G, Egée S, Esposito A, Skepper J, Tiffert T, Lew VL, Thomas SL (2010), “Effects of elevated intracellular calcium on the osmotic fragility of human red blood cells.” Cell Calcium 47(1):29-36 Details

Esposito A, Choimet JB, Skepper JN, Mauritz JM, Lew VL, Kaminski CF, Tiffert T (2010), “Quantitative imaging of human red blood cells infected with Plasmodium falciparum.” Biophys J 99(3):953-60 Details

Jeyasekharan AD, Ayoub N, Mahen R, Ries J, Esposito A, Rajendra E, Hattori H, Kulkarni RP, Venkitaraman AR (2010), “DNA damage regulates the mobility of Brca2 within the nucleoplasm of living cells.” Proc Natl Acad Sci U S A 107(50):21937-42 Details

Mauritz JM, Esposito A, Tiffert T, Skepper JN, Warley A, Yoon YZ, Cicuta P, Lew VL, Guck JR, Kaminski CF (2010), “Biophotonic techniques for the study of malaria-infected red blood cells.” Med Biol Eng Comput 48(10):1055-63 Details

Mauritz JM, Tiffert T, Seear R, Lautenschläger F, Esposito A, Lew VL, Guck J, Kaminski CF (2010), “Detection of Plasmodium falciparum-infected red blood cells by optical stretching.” J Biomed Opt 15(3):030517 Details

Swietach P, Tiffert T, Mauritz JM, Seear R, Esposito A, Kaminski CF, Lew VL, Vaughan-Jones RD (2010), “Hydrogen ion dynamics in human red blood cells.” J Physiol 588(Pt 24):4995-5014 Details

2009

Esposito A, Schlachter S, Schierle GS, Elder AD, Diaspro A, Wouters FS, Kaminski CF, Iliev AI (2009), “Quantitative fluorescence microscopy techniques.” Methods Mol Biol 586:117-42 Details

Mauritz JM, Esposito A, Ginsburg H, Kaminski CF, Tiffert T, Lew VL (2009), “The homeostasis of Plasmodium falciparum-infected red blood cells.” PLoS Comput Biol 5(4):e1000339 Details

Schlachter S, Elder AD, Esposito A, Kaminski GS, Frank JH, van Geest LK, Kaminski CF (2009), “mhFLIM: resolution of heterogeneous fluorescence decays in widefield lifetime microscopy.” Opt Express 17(3):1557-70 Details

Schlachter S, Schwedler S, Esposito A, Kaminski Schierle GS, Moggridge GD, Kaminski CF (2009), “A method to unmix multiple fluorophores in microscopy images with minimal a priori information.” Opt Express 17(25):22747-60 Details

Wagner OI, Esposito A, Köhler B, Chen CW, Shen CP, Wu GH, Butkevich E, Mandalapu S, Wenzel D, Wouters FS, Klopfenstein DR (2009), “Synaptic scaffolding protein SYD-2 clusters and activates kinesin-3 UNC-104 in C. elegans.” Proc Natl Acad Sci U S A 106(46):19605-10 Details

2008

Esposito A, Gralle M, Dani MA, Lange D, Wouters FS (2008), “pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging.” Biochemistry 47(49):13115-26 Details

Esposito A, Tiffert T, Mauritz JM, Schlachter S, Bannister LH, Kaminski CF, Lew VL (2008), “FRET imaging of hemoglobin concentration in Plasmodium falciparum-infected red cells.” PLoS One 3(11):e3780 Details

Wouters FS, Esposito A (2008), “Quantitative analysis of fluorescence lifetime imaging made easy.” HFSP J 2(1):7-11 Details

2007

Esposito A, Gerritsen HC, Wouters FS (2007), “Optimizing frequency-domain fluorescence lifetime sensing for high-throughput applications: photon economy and acquisition speed.” J Opt Soc Am A Opt Image Sci Vis 24(10):3261-73 Details

2006

Dohm CP, Siedenberg S, Liman J, Esposito A, Wouters FS, Reed JC, Bähr M, Kermer P (2006), “Bax inhibitor-1 protects neurons from oxygen-glucose deprivation.” J Mol Neurosci 29(1):1-8 Details

Esposito A, Gerritsen HC, Oggier T, Lustenberger F, Wouters FS (2006), “Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics.” J Biomed Opt 11(3):34016 Details

2005

Cupello A, Esposito A, Marchetti C, Pellistri F, Robello M (2005), “Calcium accumulation in neurites and cell bodies of rat cerebellar granule cells in culture: effects on GABA(A) receptor function.” Amino Acids 28(2):177-82 Details

Esposito A, Gerritsen HC, Wouters FS (2005), “Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis.” Biophys J 89(6):4286-99 Details

Esposito A, Robello M, Pellistri F, Marchetti C (2005), “Two-photon analysis of lead accumulation in rat cerebellar granule neurons.” Neurochem Res 30(8):949-54 Details

2004

Esposito A, Federici F, Usai C, Cannone F, Chirico G, Collini M, Diaspro A (2004), “Notes on theory and experimental conditions behind two-photon excitation microscopy.” Microsc Res Tech 63(1):12-7 Details

Esposito A, Wouters FS (2004), “Fluorescence lifetime imaging microscopy.” Curr Protoc Cell Biol Chapter 4:Unit 4.14 Details

Pellistri F, Cupello A, Esposito A, Marchetti C, Robello M (2004), “Two-photon imaging of calcium accumulation in rat cerebellar granule cells.” Neuroreport 15(1):83-7 Details