Dr Amanda Barber

Interests

Retinal ganglion cells (RGCs) convey visual signals from the retina to the brain via their axons that forms the optic nerve and the optic tract. Damage to RGC axons, as a result of injury or diseases such as glaucoma, can result in blindness as mammalian RGCs have limited intrinsic ability to regenerate their axons once injured.

The focus of my research is to investigate methods to promote RGC axon regeneration following axonal damage by injury or disease.

Adult neural retina. Cone photoreceptor cells are labelled with green fluorescent protein and immunostained for cone alpha transducin.
Adult neural retina. Cone photoreceptor cells are labelled with green fluorescent protein and immunostained for cone alpha transducin.
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Research Focus

Keywords

regeneration

axon

glacoma

vision

retinal ganglion cell

Clinical conditions

Glaucoma

Optic nerve degenerations

Equipment

Behavioural analysis

Confocal microscopy

Fluorescence microscopy

Immunohistochemistry

Microscopy

Recombinant protein expression

Collaborators

No collaborators listed

Associated News Items


    Key publications

    Barber AC, Hippert C, Duran Y, West EL, Bainbridge JW, Warre-Cornish K, Luhmann UF, Lakowski J, Sowden JC, Ali RR, Pearson RA (2013), “Repair of the degenerate retina by photoreceptor transplantation.” Proc Natl Acad Sci U S A 110(1):354-9 Details

    Pearson RA, Barber AC, Rizzi M, Hippert C, Xue T, West EL, Duran Y, Smith AJ, Chuang JZ, Azam SA, Luhmann UF, Benucci A, Sung CH, Bainbridge JW, Carandini M, Yau KW, Sowden JC, Ali RR (2012), “Restoration of vision after transplantation of photoreceptors.” Nature 485(7396):99-103 Details

    Publications

    2014

    Pearson RA, Hippert C, Graca AB, Barber AC (2014), “Photoreceptor replacement therapy: Challenges presented by the diseased recipient retinal environment.” Vis Neurosci :1-12 Details

    2013

    Warre-Cornish K, Barber AC, Sowden JC, Ali RR, Pearson RA (2013), “Migration, integration and maturation of photoreceptor precursors following transplantation in the mouse retina.” Stem Cells Dev Details

    2011

    Lakowski J, Han YT, Pearson RA, Gonzalez-Cordero A, West EL, Gualdoni S, Barber AC, Hubank M, Ali RR, Sowden JC (2011), “Effective transplantation of photoreceptor precursor cells selected via cell surface antigen expression.” Stem Cells 29(9):1391-404 Details

    2010

    Lakowski J, Baron M, Bainbridge J, Barber AC, Pearson RA, Ali RR, Sowden JC (2010), “Cone and rod photoreceptor transplantation in models of the childhood retinopathy Leber congenital amaurosis using flow-sorted Crx-positive donor cells.” Hum Mol Genet 19(23):4545-59 Details

    Pearson RA, Barber AC, West EL, MacLaren RE, Duran Y, Bainbridge JW, Sowden JC, Ali RR (2010), “Targeted disruption of outer limiting membrane junctional proteins (Crb1 and ZO-1) increases integration of transplanted photoreceptor precursors into the adult wild-type and degenerating retina.” Cell Transplant 19(4):487-503 Details

    West EL, Pearson RA, Barker SE, Luhmann UF, Maclaren RE, Barber AC, Duran Y, Smith AJ, Sowden JC, Ali RR (2010), “Long-term survival of photoreceptors transplanted into the adult murine neural retina requires immune modulation.” Stem Cells 28(11):1997-2007 Details