Mr MOHAMADY EL-GABY
Long-term potentiation (LTP) of synaptic transmission is the most prominent cellular model for mediating network changes involved in memory storage. Our lab has recently made use of optogenetic approaches to demonstrate that Schaffer collateral inputs from pyramidal cells in the left CA3 region of the mouse hippocampus onto CA1 pyramidal cells can undergo spike-timing dependent LTP (tLTP) while those from the right CA3 cannot (Kohl et al., 2011). My PhD project involves dissecting out the mechanisms underlying this asymmetry using optical and electric stimulation of Schaffer collateral inputs and recording both field and whole-cell EPSPs in CA1 pyramidal cells. I am also interested in assessing sub-cellular differences in synaptic computations and the mechanisms of LTP induction along the extensive dendritic arbour of CA1 pyramidal cells. Ultimately I would like to directly relate this input-specificity of LTP induction/maintenance to hippocampus-dependent learning and memory.
Field potential recording
Optogenetic stimulation using LEDs or lazers
Recombinant protein expression
Sterotactic gene transfer
Whole cell patch clamp
No collaborators listed
Associated News Items
El-Gaby M, Zhang Y, Wolf K, Schwiening CJ, Paulsen O, Shipton OA. (2016), “Archaerhodopsin Selectively and Reversibly Silences Synaptic Transmission through Altered pH. ” Cell reports 16(8):2259-68.
El-Gaby M, Shipton OA, Paulsen O (2014), “Synaptic Plasticity and Memory: New Insights from Hippocampal Left-Right Asymmetries.” Neuroscientist Details
Shipton OA, El-Gaby M, Apergis-Schoute J, Deisseroth K, Bannerman DM, Paulsen O, Kohl MM (2014), “Left-right dissociation of hippocampal memory processes in mice.” Proc Natl Acad Sci U S A Details